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Search of human proteins with 148839350
BLASTP 2.2.11 [Jun-05-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= gi|148839350 hypothetical protein LOC25858 [Homo sapiens]
(200 letters)
Database: hs.faa
37,866 sequences; 18,247,518 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|148839350 hypothetical protein LOC25858 [Homo sapiens] 400 e-112
gi|197333744 PCTAIRE protein kinase 1 isoform 2 [Homo sapiens] 30 1.4
gi|5453860 PCTAIRE protein kinase 1 isoform 1 [Homo sapiens] 30 1.4
gi|90652855 EF-hand calcium binding domain 5 isoform a [Homo sap... 29 2.3
gi|83035129 coiled-coil domain containing 108 isoform 1 [Homo sa... 29 2.3
gi|48762700 GTF2I repeat domain containing 2 [Homo sapiens] 29 3.0
gi|32307142 phosphatidylinositol transfer protein, cytoplasmic 1... 29 3.0
gi|32307140 phosphatidylinositol transfer protein, cytoplasmic 1... 29 3.0
gi|32455273 WNK lysine deficient protein kinase 2 [Homo sapiens] 28 4.0
gi|134948605 ankyrin repeat domain 12 isoform 2 [Homo sapiens] 28 5.2
gi|134948558 ankyrin repeat domain 12 isoform 1 [Homo sapiens] 28 5.2
gi|32307166 Rho guanine exchange factor 15 [Homo sapiens] 28 5.2
gi|239757548 PREDICTED: hypothetical protein [Homo sapiens] 28 6.8
gi|155030242 WD repeat domain 60 [Homo sapiens] 28 6.8
gi|115334679 retinoblastoma-binding protein 1 isoform III [Homo ... 28 6.8
gi|115334673 retinoblastoma-binding protein 1 isoform II [Homo s... 28 6.8
gi|115334677 retinoblastoma-binding protein 1 isoform I [Homo sa... 28 6.8
gi|90903231 huntingtin [Homo sapiens] 27 8.9
gi|70995319 nerve growth factor, beta polypeptide precursor [Hom... 27 8.9
gi|239754931 PREDICTED: hypothetical protein [Homo sapiens] 27 8.9
gi|239749473 PREDICTED: hypothetical protein XP_002347063 [Homo ... 27 8.9
gi|239743611 PREDICTED: hypothetical protein XP_002342905 [Homo ... 27 8.9
>gi|148839350 hypothetical protein LOC25858 [Homo sapiens]
Length = 200
Score = 400 bits (1029), Expect = e-112
Identities = 200/200 (100%), Positives = 200/200 (100%)
Query: 1 MEEKPSKVSLKSSDRQGSDEESVHSDTRDLWTTTTLSQAQLNMPLSEVCEGFDEEGRNIS 60
MEEKPSKVSLKSSDRQGSDEESVHSDTRDLWTTTTLSQAQLNMPLSEVCEGFDEEGRNIS
Sbjct: 1 MEEKPSKVSLKSSDRQGSDEESVHSDTRDLWTTTTLSQAQLNMPLSEVCEGFDEEGRNIS 60
Query: 61 KTRGWHSPGRGSLDEGYKASHKPEELDEHALVELELHRGSSMEINLGEKDTASQIEAEKS 120
KTRGWHSPGRGSLDEGYKASHKPEELDEHALVELELHRGSSMEINLGEKDTASQIEAEKS
Sbjct: 61 KTRGWHSPGRGSLDEGYKASHKPEELDEHALVELELHRGSSMEINLGEKDTASQIEAEKS 120
Query: 121 SSMSSLNIAKHMPHRAYWAEQQSRLPLPLMELMENEALEILTKALRSYQLGIGRDHFLTK 180
SSMSSLNIAKHMPHRAYWAEQQSRLPLPLMELMENEALEILTKALRSYQLGIGRDHFLTK
Sbjct: 121 SSMSSLNIAKHMPHRAYWAEQQSRLPLPLMELMENEALEILTKALRSYQLGIGRDHFLTK 180
Query: 181 ELQRYIEGLKKRRSKRLYVN 200
ELQRYIEGLKKRRSKRLYVN
Sbjct: 181 ELQRYIEGLKKRRSKRLYVN 200
>gi|197333744 PCTAIRE protein kinase 1 isoform 2 [Homo sapiens]
Length = 502
Score = 30.0 bits (66), Expect = 1.4
Identities = 37/120 (30%), Positives = 49/120 (40%), Gaps = 15/120 (12%)
Query: 56 GRNISKTRGWHSPGRGSLDE--GYKASHKPEELDEHALVELELHRG---SSMEI------ 104
GR I KT G +P + LDE G S E A EL RG S+ EI
Sbjct: 24 GRGIDKTNG--APEQIGLDESGGGGGSDPGEAPTRAAPGELRSARGPLSSAPEIVHEDLK 81
Query: 105 --NLGEKDTASQIEAEKSSSMSSLNIAKHMPHRAYWAEQQSRLPLPLMELMENEALEILT 162
+ GE D AS +++ S + + H P + + RL LP + LE LT
Sbjct: 82 MGSDGESDQASATSSDEVQSPVRVRMRNHPPRKISTEDINKRLSLPADIRLPEGYLEKLT 141
>gi|5453860 PCTAIRE protein kinase 1 isoform 1 [Homo sapiens]
Length = 496
Score = 30.0 bits (66), Expect = 1.4
Identities = 37/120 (30%), Positives = 49/120 (40%), Gaps = 15/120 (12%)
Query: 56 GRNISKTRGWHSPGRGSLDE--GYKASHKPEELDEHALVELELHRG---SSMEI------ 104
GR I KT G +P + LDE G S E A EL RG S+ EI
Sbjct: 18 GRGIDKTNG--APEQIGLDESGGGGGSDPGEAPTRAAPGELRSARGPLSSAPEIVHEDLK 75
Query: 105 --NLGEKDTASQIEAEKSSSMSSLNIAKHMPHRAYWAEQQSRLPLPLMELMENEALEILT 162
+ GE D AS +++ S + + H P + + RL LP + LE LT
Sbjct: 76 MGSDGESDQASATSSDEVQSPVRVRMRNHPPRKISTEDINKRLSLPADIRLPEGYLEKLT 135
>gi|90652855 EF-hand calcium binding domain 5 isoform a [Homo
sapiens]
Length = 1503
Score = 29.3 bits (64), Expect = 2.3
Identities = 31/135 (22%), Positives = 55/135 (40%), Gaps = 1/135 (0%)
Query: 54 EEGRNISKTRGWHSPGRGSLDEGYKA-SHKPEELDEHALVELELHRGSSMEINLGEKDTA 112
+E R K R W+ L E ++ P + D +++VE + S E+NL +
Sbjct: 14 QENRKEDKERKWNLTEVKELHETLQSVPDVPVKEDTNSVVEKAMDEIKSQELNLEGQRKI 73
Query: 113 SQIEAEKSSSMSSLNIAKHMPHRAYWAEQQSRLPLPLMELMENEALEILTKALRSYQLGI 172
S + S + +S NIA + +A ++ L + E + ++ Q I
Sbjct: 74 SPGSIKDSKTEASGNIAIRKSAKVIFALDETELKSKPEHTWKKNLFERMEARAQAMQQKI 133
Query: 173 GRDHFLTKELQRYIE 187
L KEL++ E
Sbjct: 134 IDKENLKKELEKKAE 148
>gi|83035129 coiled-coil domain containing 108 isoform 1 [Homo
sapiens]
Length = 1925
Score = 29.3 bits (64), Expect = 2.3
Identities = 21/71 (29%), Positives = 31/71 (43%), Gaps = 2/71 (2%)
Query: 53 DEEGRNISKTRGWHSPGRGSLDEG--YKASHKPEELDEHALVELELHRGSSMEINLGEKD 110
D +G+ + R H PG G +EG K + EEL+E E E + + EK+
Sbjct: 1740 DGKGKQPKEDRPEHYPGLGKKEEGEEEKGEEEEEELEEEEEEEEETEEEELGKEEIEEKE 1799
Query: 111 TASQIEAEKSS 121
+ EK S
Sbjct: 1800 EERDEKEEKVS 1810
>gi|48762700 GTF2I repeat domain containing 2 [Homo sapiens]
Length = 949
Score = 28.9 bits (63), Expect = 3.0
Identities = 16/48 (33%), Positives = 25/48 (52%)
Query: 125 SLNIAKHMPHRAYWAEQQSRLPLPLMELMENEALEILTKALRSYQLGI 172
S++++K R ++ S+ ME M +E L L K LR Y LG+
Sbjct: 453 SMSVSKEYNLRRHYQTNHSKHYDQYMERMRDEKLHELKKGLRKYLLGL 500
>gi|32307142 phosphatidylinositol transfer protein, cytoplasmic 1
isoform b [Homo sapiens]
Length = 268
Score = 28.9 bits (63), Expect = 3.0
Identities = 17/76 (22%), Positives = 36/76 (47%), Gaps = 5/76 (6%)
Query: 8 VSLKSSDRQGSDEESVHSDTRDLWTTTTLSQAQLNMPLSEVCEGFDEEGRNISKTRGWHS 67
+ K D +GS++ ++ +D+ +++ E+ E + +E + +
Sbjct: 109 IETKYEDNKGSNDTIFDNEAKDVEREVCF----IDIACDEIPERYYKESEDPKHFKS-EK 163
Query: 68 PGRGSLDEGYKASHKP 83
GRG L EG++ SH+P
Sbjct: 164 TGRGQLREGWRDSHQP 179
>gi|32307140 phosphatidylinositol transfer protein, cytoplasmic 1
isoform a [Homo sapiens]
Length = 332
Score = 28.9 bits (63), Expect = 3.0
Identities = 17/76 (22%), Positives = 36/76 (47%), Gaps = 5/76 (6%)
Query: 8 VSLKSSDRQGSDEESVHSDTRDLWTTTTLSQAQLNMPLSEVCEGFDEEGRNISKTRGWHS 67
+ K D +GS++ ++ +D+ +++ E+ E + +E + +
Sbjct: 109 IETKYEDNKGSNDTIFDNEAKDVEREVCF----IDIACDEIPERYYKESEDPKHFKS-EK 163
Query: 68 PGRGSLDEGYKASHKP 83
GRG L EG++ SH+P
Sbjct: 164 TGRGQLREGWRDSHQP 179
>gi|32455273 WNK lysine deficient protein kinase 2 [Homo sapiens]
Length = 2217
Score = 28.5 bits (62), Expect = 4.0
Identities = 15/58 (25%), Positives = 34/58 (58%), Gaps = 3/58 (5%)
Query: 12 SSDRQGSDEESVHSDTRDLWTTTTLSQAQLNMPLSEVCEGFDEEGRNISKTRGWHSPG 69
S++++G+ + +H D WT+ T+ AQL L+++ + ++ +++ GW +PG
Sbjct: 2114 SNNKKGTFTDDLHKLV-DEWTSKTVGAAQLKPTLNQLKQ--TQKLQDMEAQAGWAAPG 2168
>gi|134948605 ankyrin repeat domain 12 isoform 2 [Homo sapiens]
Length = 2039
Score = 28.1 bits (61), Expect = 5.2
Identities = 29/129 (22%), Positives = 54/129 (41%), Gaps = 4/129 (3%)
Query: 2 EEKPSKVS--LKSSDRQGSDEESVHSDTRDLWTTTTLSQAQLNMPLSEVCEGFDEEGRNI 59
+EKP K +S ++ ++++ SD + + + + L + + D+E NI
Sbjct: 890 KEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINI 949
Query: 60 SKTRGWHSPGRGSLDEGYKASH-KPEELDEHALVELELHRGSSMEINLGEKDTASQIEAE 118
+ ++ + S+ +G KA H KP L E E L E + +KD E +
Sbjct: 950 TNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDE-PLKTPDGKEKDKKDKDIDRYKERD 1008
Query: 119 KSSSMSSLN 127
K +N
Sbjct: 1009 KHKDKIQIN 1017
>gi|134948558 ankyrin repeat domain 12 isoform 1 [Homo sapiens]
Length = 2062
Score = 28.1 bits (61), Expect = 5.2
Identities = 29/129 (22%), Positives = 54/129 (41%), Gaps = 4/129 (3%)
Query: 2 EEKPSKVS--LKSSDRQGSDEESVHSDTRDLWTTTTLSQAQLNMPLSEVCEGFDEEGRNI 59
+EKP K +S ++ ++++ SD + + + + L + + D+E NI
Sbjct: 913 KEKPEKERHLAESKEKHLMEKKNKQSDNSEYSKSEKGKNKEKDRELDKKEKSRDKESINI 972
Query: 60 SKTRGWHSPGRGSLDEGYKASH-KPEELDEHALVELELHRGSSMEINLGEKDTASQIEAE 118
+ ++ + S+ +G KA H KP L E E L E + +KD E +
Sbjct: 973 TNSKHIQEEKKSSIVDGNKAQHEKPLSLKEKTKDE-PLKTPDGKEKDKKDKDIDRYKERD 1031
Query: 119 KSSSMSSLN 127
K +N
Sbjct: 1032 KHKDKIQIN 1040
>gi|32307166 Rho guanine exchange factor 15 [Homo sapiens]
Length = 841
Score = 28.1 bits (61), Expect = 5.2
Identities = 41/167 (24%), Positives = 70/167 (41%), Gaps = 21/167 (12%)
Query: 38 QAQLNMPLSEVCEGFDEEGRNISKTRGWHSPGRGS-LDEGYKASHKPEELDEHALVELEL 96
+A N P + V EG +EEG + K + W P + L + Y+A+ EEL
Sbjct: 317 EAPQNTPPATV-EGREEEGLEVLKEQNWELPLQDEPLYQTYRAAVLSEELWGVG------ 369
Query: 97 HRGSSMEINLGEKDTASQIEAEKSSSMSSLNIAKHMPHRAYWAEQQSRLPLPLMELMENE 156
GS N G+ T + +++ L + + Q+ R+ L E++ +E
Sbjct: 370 EDGSPSPANAGDAPTFPRPPGPRNTLWQELPAVQASGLLDTLSPQERRMQESLFEVVTSE 429
Query: 157 A-----------LEILTKALRSYQLGIGRDHFLTKELQRYIEGLKKR 192
A +L++ALR L H L +QR ++G+ +R
Sbjct: 430 ASYLRSLRLLTDTFVLSQALRD-TLTPRDHHTLFSNVQR-VQGVSER 474
>gi|239757548 PREDICTED: hypothetical protein [Homo sapiens]
Length = 132
Score = 27.7 bits (60), Expect = 6.8
Identities = 11/18 (61%), Positives = 13/18 (72%)
Query: 133 PHRAYWAEQQSRLPLPLM 150
P R +WAE +SR PLP M
Sbjct: 54 PWRPWWAENKSRRPLPSM 71
>gi|155030242 WD repeat domain 60 [Homo sapiens]
Length = 1066
Score = 27.7 bits (60), Expect = 6.8
Identities = 43/208 (20%), Positives = 77/208 (37%), Gaps = 26/208 (12%)
Query: 1 MEEKPSKVSLKSSDRQGSDEESVHSDTRDLWTTTTLSQAQLNMPLSEVCEGFDEEGRNIS 60
++++P +++ +D E+ +D +T + + EVC+G D+E N
Sbjct: 343 LDQRPGGEETVEIEKEETDLENARADA---YTASCEDDFEDYEDDFEVCDGDDDESSN-- 397
Query: 61 KTRGWHSPGRGSLDEGYKASHKPEELDEHALVELELHRGSSMEINLGEKDTASQIEAE-- 118
R L+E + K E + + E R + + L +K ++ E E
Sbjct: 398 -----EPESREKLEE-LPLAQKKEIQEIQRAINAENERIGELSLKLFQKRGRTEFEKEPR 451
Query: 119 --KSSSMSSLNIAKHMPHRAYWAEQQSRLPLPLMELMENEALEILTKALRSYQLGIGRDH 176
+SS S ++ A + +Q L + M + TK LR L
Sbjct: 452 TDTNSSPSRASVCGIFVDFASASHRQKSRTQALKQKMRS------TKLLRLIDLDFSFTF 505
Query: 177 FL-----TKELQRYIEGLKKRRSKRLYV 199
L E YI K+ +K+ YV
Sbjct: 506 SLLDLPPVNEYDMYIRNFGKKNTKQAYV 533
>gi|115334679 retinoblastoma-binding protein 1 isoform III [Homo
sapiens]
Length = 1188
Score = 27.7 bits (60), Expect = 6.8
Identities = 23/98 (23%), Positives = 43/98 (43%), Gaps = 9/98 (9%)
Query: 2 EEKPSKVSLKSSDRQGSDEESVHSDTRDLWTTTTLSQAQLNMPLSEVCEGFDEEGRNISK 61
++K K+ + + SDEE S R+ + S+ + + E C ++
Sbjct: 526 KQKEKKIKKQEDSDKDSDEEEEKSQEREETESKCDSEGEEDEEDMEPC---------LTG 576
Query: 62 TRGWHSPGRGSLDEGYKASHKPEELDEHALVELELHRG 99
T+ GRG + Y+AS K E+D+ ++ L + G
Sbjct: 577 TKVKVKYGRGKTQKIYEASIKSTEIDDGEVLYLVHYYG 614
>gi|115334673 retinoblastoma-binding protein 1 isoform II [Homo
sapiens]
Length = 1203
Score = 27.7 bits (60), Expect = 6.8
Identities = 23/98 (23%), Positives = 43/98 (43%), Gaps = 9/98 (9%)
Query: 2 EEKPSKVSLKSSDRQGSDEESVHSDTRDLWTTTTLSQAQLNMPLSEVCEGFDEEGRNISK 61
++K K+ + + SDEE S R+ + S+ + + E C ++
Sbjct: 526 KQKEKKIKKQEDSDKDSDEEEEKSQEREETESKCDSEGEEDEEDMEPC---------LTG 576
Query: 62 TRGWHSPGRGSLDEGYKASHKPEELDEHALVELELHRG 99
T+ GRG + Y+AS K E+D+ ++ L + G
Sbjct: 577 TKVKVKYGRGKTQKIYEASIKSTEIDDGEVLYLVHYYG 614
>gi|115334677 retinoblastoma-binding protein 1 isoform I [Homo
sapiens]
Length = 1257
Score = 27.7 bits (60), Expect = 6.8
Identities = 23/98 (23%), Positives = 43/98 (43%), Gaps = 9/98 (9%)
Query: 2 EEKPSKVSLKSSDRQGSDEESVHSDTRDLWTTTTLSQAQLNMPLSEVCEGFDEEGRNISK 61
++K K+ + + SDEE S R+ + S+ + + E C ++
Sbjct: 526 KQKEKKIKKQEDSDKDSDEEEEKSQEREETESKCDSEGEEDEEDMEPC---------LTG 576
Query: 62 TRGWHSPGRGSLDEGYKASHKPEELDEHALVELELHRG 99
T+ GRG + Y+AS K E+D+ ++ L + G
Sbjct: 577 TKVKVKYGRGKTQKIYEASIKSTEIDDGEVLYLVHYYG 614
>gi|90903231 huntingtin [Homo sapiens]
Length = 3144
Score = 27.3 bits (59), Expect = 8.9
Identities = 17/52 (32%), Positives = 24/52 (46%), Gaps = 3/52 (5%)
Query: 82 KPEELDEHALVELELHRGSSMEINLGEKDTASQIEAEKSSSMSSLNIAKHMP 133
K +E E A V L +GS + DT+ + KSSS+ S H+P
Sbjct: 1188 KEKEPGEQASVPLSPKKGSEASAASRQSDTSGPVTTSKSSSLGSF---YHLP 1236
>gi|70995319 nerve growth factor, beta polypeptide precursor [Homo
sapiens]
Length = 241
Score = 27.3 bits (59), Expect = 8.9
Identities = 18/78 (23%), Positives = 38/78 (48%), Gaps = 10/78 (12%)
Query: 77 YKASHKPEELDEHALVELELHRGS-----SMEINLGEKDTASQIEAEKSSSMSSLNIAKH 131
+ +H+ + H + HRG S+ + +G+K TA+ I+ ++ + +NI +
Sbjct: 113 FNRTHRSKRSSSHPI----FHRGEFSVCDSVSVWVGDKTTATDIKGKEVMVLGEVNI-NN 167
Query: 132 MPHRAYWAEQQSRLPLPL 149
+ Y+ E + R P P+
Sbjct: 168 SVFKQYFFETKCRDPNPV 185
>gi|239754931 PREDICTED: hypothetical protein [Homo sapiens]
Length = 119
Score = 27.3 bits (59), Expect = 8.9
Identities = 12/31 (38%), Positives = 17/31 (54%)
Query: 53 DEEGRNISKTRGWHSPGRGSLDEGYKASHKP 83
+E GR++ K H GRG L+E +H P
Sbjct: 37 NEAGRDLEKPPRLHCSGRGRLEEPVPPNHLP 67
>gi|239749473 PREDICTED: hypothetical protein XP_002347063 [Homo
sapiens]
Length = 119
Score = 27.3 bits (59), Expect = 8.9
Identities = 12/31 (38%), Positives = 17/31 (54%)
Query: 53 DEEGRNISKTRGWHSPGRGSLDEGYKASHKP 83
+E GR++ K H GRG L+E +H P
Sbjct: 37 NEAGRDLEKPPRLHCSGRGRLEEPVPPNHLP 67
>gi|239743611 PREDICTED: hypothetical protein XP_002342905 [Homo
sapiens]
Length = 119
Score = 27.3 bits (59), Expect = 8.9
Identities = 12/31 (38%), Positives = 17/31 (54%)
Query: 53 DEEGRNISKTRGWHSPGRGSLDEGYKASHKP 83
+E GR++ K H GRG L+E +H P
Sbjct: 37 NEAGRDLEKPPRLHCSGRGRLEEPVPPNHLP 67
Database: hs.faa
Posted date: Aug 4, 2009 4:42 PM
Number of letters in database: 18,247,518
Number of sequences in database: 37,866
Lambda K H
0.310 0.126 0.354
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,293,815
Number of Sequences: 37866
Number of extensions: 302179
Number of successful extensions: 740
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 16
Number of HSP's that attempted gapping in prelim test: 732
Number of HSP's gapped (non-prelim): 24
length of query: 200
length of database: 18,247,518
effective HSP length: 97
effective length of query: 103
effective length of database: 14,574,516
effective search space: 1501175148
effective search space used: 1501175148
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 59 (27.3 bits)
Search results were obtained with NCBI BLAST and RefSeq entries.
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Guide to the Human Genome
Copyright © 2010 by Stewart Scherer. All rights reserved.