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Search of human proteins with 21362032
BLASTP 2.2.11 [Jun-05-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= gi|21362032 ELMO/CED-12 domain containing 3 isoform a [Homo
sapiens]
(391 letters)
Database: hs.faa
37,866 sequences; 18,247,518 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|21362032 ELMO/CED-12 domain containing 3 isoform a [Homo sapi... 790 0.0
gi|203098650 ELMO/CED-12 domain containing 3 isoform b [Homo sap... 636 0.0
gi|203098539 ELMO/CED-12 domain containing 3 isoform b [Homo sap... 636 0.0
gi|203098474 ELMO/CED-12 domain containing 3 isoform b [Homo sap... 636 0.0
gi|24308456 ELMO/CED-12 domain containing 2 [Homo sapiens] 60 3e-09
gi|194097484 ELMO/CED-12 domain containing 1 isoform 2 [Homo sap... 48 1e-05
gi|112181294 ELMO/CED-12 domain containing 1 isoform 1 [Homo sap... 47 3e-05
gi|87298935 engulfment and cell motility 3 [Homo sapiens] 37 0.023
gi|154426331 early undifferentiated retina and lens isoform 1 [H... 32 0.75
gi|110347463 human immunodeficiency virus type I enhancer bindin... 32 0.98
gi|18765700 engulfment and cell motility 1 isoform 1 [Homo sapiens] 31 1.7
gi|116517321 olfactory receptor, family 52, subfamily R, member ... 30 3.7
gi|169173146 PREDICTED: hypothetical protein [Homo sapiens] 30 4.9
gi|113419909 PREDICTED: hypothetical protein [Homo sapiens] 30 4.9
gi|23592220 G protein-coupled receptor 26 [Homo sapiens] 29 8.3
>gi|21362032 ELMO/CED-12 domain containing 3 isoform a [Homo
sapiens]
Length = 391
Score = 790 bits (2040), Expect = 0.0
Identities = 391/391 (100%), Positives = 391/391 (100%)
Query: 1 MNEKSCSFHSKEELRDGQGERLSAGYSPSYDKDKSVLAFRGIPISELKNHGILQALTTEA 60
MNEKSCSFHSKEELRDGQGERLSAGYSPSYDKDKSVLAFRGIPISELKNHGILQALTTEA
Sbjct: 1 MNEKSCSFHSKEELRDGQGERLSAGYSPSYDKDKSVLAFRGIPISELKNHGILQALTTEA 60
Query: 61 YEWEPRVVSTEVVRAQEEWEAVDTIQPETGSQASSEQPGQLISFSEALQHFQTVDLSPFK 120
YEWEPRVVSTEVVRAQEEWEAVDTIQPETGSQASSEQPGQLISFSEALQHFQTVDLSPFK
Sbjct: 61 YEWEPRVVSTEVVRAQEEWEAVDTIQPETGSQASSEQPGQLISFSEALQHFQTVDLSPFK 120
Query: 121 KRIQPTIRRTGLAALRHYLFGPPKLHQRLREERDLVLTIAQCGLDSQDPVHGRVLQTIYK 180
KRIQPTIRRTGLAALRHYLFGPPKLHQRLREERDLVLTIAQCGLDSQDPVHGRVLQTIYK
Sbjct: 121 KRIQPTIRRTGLAALRHYLFGPPKLHQRLREERDLVLTIAQCGLDSQDPVHGRVLQTIYK 180
Query: 181 KLTGSKFDCALHGNHWEDLGFQGANPATDLRGAGFLALLHLLYLVMDSKTLPMAQEIFRL 240
KLTGSKFDCALHGNHWEDLGFQGANPATDLRGAGFLALLHLLYLVMDSKTLPMAQEIFRL
Sbjct: 181 KLTGSKFDCALHGNHWEDLGFQGANPATDLRGAGFLALLHLLYLVMDSKTLPMAQEIFRL 240
Query: 241 SRHHIQQFPFCLMSVNITHIAIQALREECLSRECNRQQKVIPVVNSFYAATFLHLAHVWR 300
SRHHIQQFPFCLMSVNITHIAIQALREECLSRECNRQQKVIPVVNSFYAATFLHLAHVWR
Sbjct: 241 SRHHIQQFPFCLMSVNITHIAIQALREECLSRECNRQQKVIPVVNSFYAATFLHLAHVWR 300
Query: 301 TQRKTISDSGFVLKGVLFLLGRPRLNAQCPRSREPKVVARLVLAAVLPHPHFLKFQLTKI 360
TQRKTISDSGFVLKGVLFLLGRPRLNAQCPRSREPKVVARLVLAAVLPHPHFLKFQLTKI
Sbjct: 301 TQRKTISDSGFVLKGVLFLLGRPRLNAQCPRSREPKVVARLVLAAVLPHPHFLKFQLTKI 360
Query: 361 SITHPLESASSPFSALTVALFWSYTYDKHIF 391
SITHPLESASSPFSALTVALFWSYTYDKHIF
Sbjct: 361 SITHPLESASSPFSALTVALFWSYTYDKHIF 391
>gi|203098650 ELMO/CED-12 domain containing 3 isoform b [Homo
sapiens]
Length = 381
Score = 636 bits (1640), Expect = 0.0
Identities = 314/314 (100%), Positives = 314/314 (100%)
Query: 1 MNEKSCSFHSKEELRDGQGERLSAGYSPSYDKDKSVLAFRGIPISELKNHGILQALTTEA 60
MNEKSCSFHSKEELRDGQGERLSAGYSPSYDKDKSVLAFRGIPISELKNHGILQALTTEA
Sbjct: 1 MNEKSCSFHSKEELRDGQGERLSAGYSPSYDKDKSVLAFRGIPISELKNHGILQALTTEA 60
Query: 61 YEWEPRVVSTEVVRAQEEWEAVDTIQPETGSQASSEQPGQLISFSEALQHFQTVDLSPFK 120
YEWEPRVVSTEVVRAQEEWEAVDTIQPETGSQASSEQPGQLISFSEALQHFQTVDLSPFK
Sbjct: 61 YEWEPRVVSTEVVRAQEEWEAVDTIQPETGSQASSEQPGQLISFSEALQHFQTVDLSPFK 120
Query: 121 KRIQPTIRRTGLAALRHYLFGPPKLHQRLREERDLVLTIAQCGLDSQDPVHGRVLQTIYK 180
KRIQPTIRRTGLAALRHYLFGPPKLHQRLREERDLVLTIAQCGLDSQDPVHGRVLQTIYK
Sbjct: 121 KRIQPTIRRTGLAALRHYLFGPPKLHQRLREERDLVLTIAQCGLDSQDPVHGRVLQTIYK 180
Query: 181 KLTGSKFDCALHGNHWEDLGFQGANPATDLRGAGFLALLHLLYLVMDSKTLPMAQEIFRL 240
KLTGSKFDCALHGNHWEDLGFQGANPATDLRGAGFLALLHLLYLVMDSKTLPMAQEIFRL
Sbjct: 181 KLTGSKFDCALHGNHWEDLGFQGANPATDLRGAGFLALLHLLYLVMDSKTLPMAQEIFRL 240
Query: 241 SRHHIQQFPFCLMSVNITHIAIQALREECLSRECNRQQKVIPVVNSFYAATFLHLAHVWR 300
SRHHIQQFPFCLMSVNITHIAIQALREECLSRECNRQQKVIPVVNSFYAATFLHLAHVWR
Sbjct: 241 SRHHIQQFPFCLMSVNITHIAIQALREECLSRECNRQQKVIPVVNSFYAATFLHLAHVWR 300
Query: 301 TQRKTISDSGFVLK 314
TQRKTISDSGFVLK
Sbjct: 301 TQRKTISDSGFVLK 314
>gi|203098539 ELMO/CED-12 domain containing 3 isoform b [Homo
sapiens]
Length = 381
Score = 636 bits (1640), Expect = 0.0
Identities = 314/314 (100%), Positives = 314/314 (100%)
Query: 1 MNEKSCSFHSKEELRDGQGERLSAGYSPSYDKDKSVLAFRGIPISELKNHGILQALTTEA 60
MNEKSCSFHSKEELRDGQGERLSAGYSPSYDKDKSVLAFRGIPISELKNHGILQALTTEA
Sbjct: 1 MNEKSCSFHSKEELRDGQGERLSAGYSPSYDKDKSVLAFRGIPISELKNHGILQALTTEA 60
Query: 61 YEWEPRVVSTEVVRAQEEWEAVDTIQPETGSQASSEQPGQLISFSEALQHFQTVDLSPFK 120
YEWEPRVVSTEVVRAQEEWEAVDTIQPETGSQASSEQPGQLISFSEALQHFQTVDLSPFK
Sbjct: 61 YEWEPRVVSTEVVRAQEEWEAVDTIQPETGSQASSEQPGQLISFSEALQHFQTVDLSPFK 120
Query: 121 KRIQPTIRRTGLAALRHYLFGPPKLHQRLREERDLVLTIAQCGLDSQDPVHGRVLQTIYK 180
KRIQPTIRRTGLAALRHYLFGPPKLHQRLREERDLVLTIAQCGLDSQDPVHGRVLQTIYK
Sbjct: 121 KRIQPTIRRTGLAALRHYLFGPPKLHQRLREERDLVLTIAQCGLDSQDPVHGRVLQTIYK 180
Query: 181 KLTGSKFDCALHGNHWEDLGFQGANPATDLRGAGFLALLHLLYLVMDSKTLPMAQEIFRL 240
KLTGSKFDCALHGNHWEDLGFQGANPATDLRGAGFLALLHLLYLVMDSKTLPMAQEIFRL
Sbjct: 181 KLTGSKFDCALHGNHWEDLGFQGANPATDLRGAGFLALLHLLYLVMDSKTLPMAQEIFRL 240
Query: 241 SRHHIQQFPFCLMSVNITHIAIQALREECLSRECNRQQKVIPVVNSFYAATFLHLAHVWR 300
SRHHIQQFPFCLMSVNITHIAIQALREECLSRECNRQQKVIPVVNSFYAATFLHLAHVWR
Sbjct: 241 SRHHIQQFPFCLMSVNITHIAIQALREECLSRECNRQQKVIPVVNSFYAATFLHLAHVWR 300
Query: 301 TQRKTISDSGFVLK 314
TQRKTISDSGFVLK
Sbjct: 301 TQRKTISDSGFVLK 314
>gi|203098474 ELMO/CED-12 domain containing 3 isoform b [Homo
sapiens]
Length = 381
Score = 636 bits (1640), Expect = 0.0
Identities = 314/314 (100%), Positives = 314/314 (100%)
Query: 1 MNEKSCSFHSKEELRDGQGERLSAGYSPSYDKDKSVLAFRGIPISELKNHGILQALTTEA 60
MNEKSCSFHSKEELRDGQGERLSAGYSPSYDKDKSVLAFRGIPISELKNHGILQALTTEA
Sbjct: 1 MNEKSCSFHSKEELRDGQGERLSAGYSPSYDKDKSVLAFRGIPISELKNHGILQALTTEA 60
Query: 61 YEWEPRVVSTEVVRAQEEWEAVDTIQPETGSQASSEQPGQLISFSEALQHFQTVDLSPFK 120
YEWEPRVVSTEVVRAQEEWEAVDTIQPETGSQASSEQPGQLISFSEALQHFQTVDLSPFK
Sbjct: 61 YEWEPRVVSTEVVRAQEEWEAVDTIQPETGSQASSEQPGQLISFSEALQHFQTVDLSPFK 120
Query: 121 KRIQPTIRRTGLAALRHYLFGPPKLHQRLREERDLVLTIAQCGLDSQDPVHGRVLQTIYK 180
KRIQPTIRRTGLAALRHYLFGPPKLHQRLREERDLVLTIAQCGLDSQDPVHGRVLQTIYK
Sbjct: 121 KRIQPTIRRTGLAALRHYLFGPPKLHQRLREERDLVLTIAQCGLDSQDPVHGRVLQTIYK 180
Query: 181 KLTGSKFDCALHGNHWEDLGFQGANPATDLRGAGFLALLHLLYLVMDSKTLPMAQEIFRL 240
KLTGSKFDCALHGNHWEDLGFQGANPATDLRGAGFLALLHLLYLVMDSKTLPMAQEIFRL
Sbjct: 181 KLTGSKFDCALHGNHWEDLGFQGANPATDLRGAGFLALLHLLYLVMDSKTLPMAQEIFRL 240
Query: 241 SRHHIQQFPFCLMSVNITHIAIQALREECLSRECNRQQKVIPVVNSFYAATFLHLAHVWR 300
SRHHIQQFPFCLMSVNITHIAIQALREECLSRECNRQQKVIPVVNSFYAATFLHLAHVWR
Sbjct: 241 SRHHIQQFPFCLMSVNITHIAIQALREECLSRECNRQQKVIPVVNSFYAATFLHLAHVWR 300
Query: 301 TQRKTISDSGFVLK 314
TQRKTISDSGFVLK
Sbjct: 301 TQRKTISDSGFVLK 314
>gi|24308456 ELMO/CED-12 domain containing 2 [Homo sapiens]
Length = 293
Score = 60.1 bits (144), Expect = 3e-09
Identities = 35/124 (28%), Positives = 57/124 (45%), Gaps = 2/124 (1%)
Query: 165 DSQDPVHGRVLQTIYKKLTGSKFDCALHGNHWEDLGFQGANPATDLRGAGFLALLHLLYL 224
DS + H +L ++ L +K A W ++GFQG +P TD RG G L L++L+Y
Sbjct: 121 DSDNLQHEELLMKLWNLLMPTKKLNARISKQWAEIGFQGDDPKTDFRGMGILGLINLVY- 179
Query: 225 VMDSKTLPMAQEIFRLSRHHIQQFPFCLMSVNITHIAIQALREECLSRECNRQQKVIPVV 284
A +I S H + + ++ +N+T +A L+ E L IP +
Sbjct: 180 -FSENYTSEAHQILSRSNHPKLGYSYAIVGINLTEMAYSLLKSEALKFHLYNLVPGIPTM 238
Query: 285 NSFY 288
F+
Sbjct: 239 EHFH 242
>gi|194097484 ELMO/CED-12 domain containing 1 isoform 2 [Homo
sapiens]
Length = 326
Score = 48.1 bits (113), Expect = 1e-05
Identities = 40/150 (26%), Positives = 60/150 (40%), Gaps = 20/150 (13%)
Query: 165 DSQDPVHGRVLQTIYKKLTGSKFDCALHGNHWEDLGFQGANPATDLRGAGFLALLHLLYL 224
DS +P H +L ++K L + + W ++GFQG +P TD RG G L L +L Y
Sbjct: 128 DSDNPQHEEMLLKLWKFLKPNTPLESRISKQWCEIGFQGDDPKTDFRGMGLLGLYNLQYF 187
Query: 225 VMDSKTLPMAQEIFRLSRH-----------------HIQQFPFCLMSVNITHIAIQALRE 267
T AQ++ S H + F ++ +NIT +A L
Sbjct: 188 AERDAT--AAQQVLSDSLHPKCSKFSKAEWEKKRMDKAIGYSFAIVGINITDLAYNLLVS 245
Query: 268 ECLSRECNRQQKVIPVVNSFYAATFLHLAH 297
L P + S + TF +L H
Sbjct: 246 GALKTHFYNIAPEAPTL-SHFQQTFCYLMH 274
>gi|112181294 ELMO/CED-12 domain containing 1 isoform 1 [Homo
sapiens]
Length = 334
Score = 47.0 bits (110), Expect = 3e-05
Identities = 39/156 (25%), Positives = 62/156 (39%), Gaps = 24/156 (15%)
Query: 165 DSQDPVHGRVLQTIYKKLTGSKFDCALHGNHWEDLGFQGANPATDLRGAGFLALLHLLYL 224
DS +P H +L ++K L + + W ++GFQG +P TD RG G L L +L Y
Sbjct: 128 DSDNPQHEEMLLKLWKFLKPNTPLESRISKQWCEIGFQGDDPKTDFRGMGLLGLYNLQYF 187
Query: 225 VMDSKTLPM---------------AQEIFRLSRHHIQQ--------FPFCLMSVNITHIA 261
T +EI + S+ ++ + F ++ +NIT +A
Sbjct: 188 AERDATAAQQVLSDSLHPKCRDITKEEISKFSKAEWEKKRMDKAIGYSFAIVGINITDLA 247
Query: 262 IQALREECLSRECNRQQKVIPVVNSFYAATFLHLAH 297
L L P + S + TF +L H
Sbjct: 248 YNLLVSGALKTHFYNIAPEAPTL-SHFQQTFCYLMH 282
>gi|87298935 engulfment and cell motility 3 [Homo sapiens]
Length = 773
Score = 37.4 bits (85), Expect = 0.023
Identities = 46/183 (25%), Positives = 71/183 (38%), Gaps = 21/183 (11%)
Query: 150 REERDLVLTIAQCGLDSQDPVHGRVLQTIYKKLTGSKFDCALHGNHWEDLGFQGANPATD 209
+E+R+ + + Q + + G L ++ +L + LGF +NPA D
Sbjct: 359 QEQREQLQVLRQAAFEVEGESSGAGLSADRRR--------SLCAREFRKLGFSNSNPAQD 410
Query: 210 LRGA--GFLALLHLLYLVMDSKTLPMAQEIFRL---SRHHIQQFPFCLMSVNITHIAIQA 264
L G LAL ++LY S+ P A F L SR + PF S+ +T + +
Sbjct: 411 LERVPPGLLALDNMLYF---SRNAPSAYSRFVLENSSREDKHECPFARGSIQLTVLLCEL 467
Query: 265 LR--EECLSRECNRQQKVIPVVNSF---YAATFLHLAHVWRTQRKTISDSGFVLKGVLFL 319
LR E C + SF + L W+ R T D V++ V
Sbjct: 468 LRVGEPCSETAQDFSPMFFGQDQSFHELFCVGIQLLNKTWKEMRATQEDFDKVMQVVREQ 527
Query: 320 LGR 322
L R
Sbjct: 528 LAR 530
>gi|154426331 early undifferentiated retina and lens isoform 1 [Homo
sapiens]
Length = 297
Score = 32.3 bits (72), Expect = 0.75
Identities = 13/49 (26%), Positives = 25/49 (51%)
Query: 226 MDSKTLPMAQEIFRLSRHHIQQFPFCLMSVNITHIAIQALREECLSREC 274
+++K L QE+F H +Q+ +++ H+AI+ L + C C
Sbjct: 233 LNAKLLQQIQEVFEELTHQVQEKDSLASQLHVRHVAIEQLLKNCSKLPC 281
>gi|110347463 human immunodeficiency virus type I enhancer binding
protein 2 [Homo sapiens]
Length = 2446
Score = 32.0 bits (71), Expect = 0.98
Identities = 20/84 (23%), Positives = 41/84 (48%), Gaps = 1/84 (1%)
Query: 54 QALTTEAYEWEPRVVSTEVVRAQEEWEAVDTIQPETGSQASSEQPGQLISFSEALQHFQT 113
Q + ++Y +PR+V ++ E + +PE +A S++P + + + Q +T
Sbjct: 867 QQVQQQSYHTQPRLVRQHNIQVPEIRVTEEPDKPEKEKEAQSKEPEKPVEEFQWPQRSET 926
Query: 114 VDLSPFKKRIQPTIRRTGLAALRH 137
+ P +K + P +R LA + H
Sbjct: 927 LSQLPAEK-LPPKKKRLRLADMEH 949
>gi|18765700 engulfment and cell motility 1 isoform 1 [Homo sapiens]
Length = 727
Score = 31.2 bits (69), Expect = 1.7
Identities = 37/181 (20%), Positives = 70/181 (38%), Gaps = 16/181 (8%)
Query: 144 KLHQRLREERDLVLTIAQCGLDSQDPVHGRVLQTIYKKLTGSKFDCALHGNHWEDLGF-Q 202
K+ + + +RD++ + + D++ + +K +++ ++ LGF
Sbjct: 312 KMDPQDQAQRDIIFELRRIAFDAESEPNNSSGSMEKRK--------SMYTRDYKKLGFIN 363
Query: 203 GANPATDLRGA--GFLALLHLLYLVMDSKTLPMAQEIFRLSRHHIQQFPFCLMSVNITHI 260
NPA D G LAL ++LY + + + SR + PF S+ +T +
Sbjct: 364 HVNPAMDFTQTPPGMLALDNMLYFAKHHQDAYIRIVLENSSREDKHECPFGRSSIELTKM 423
Query: 261 AIQALR-EECLSRECNRQQKVI----PVVNSFYAATFLHLAHVWRTQRKTISDSGFVLKG 315
+ L+ E S CN + F+ L W+ R T D V++
Sbjct: 424 LCEILKVGELPSETCNDFHPMFFTHDRSFEEFFCICIQLLNKTWKEMRATSEDFNKVMQV 483
Query: 316 V 316
V
Sbjct: 484 V 484
>gi|116517321 olfactory receptor, family 52, subfamily R, member 1
[Homo sapiens]
Length = 394
Score = 30.0 bits (66), Expect = 3.7
Identities = 18/45 (40%), Positives = 23/45 (51%), Gaps = 1/45 (2%)
Query: 215 FLALLHLLYLVMDSKTLPMAQEIFRLSRHHIQQFPFCLMSVNITH 259
FLA+L + LV+ S T P IF H I Q+ CL+ V H
Sbjct: 144 FLAMLAITDLVLSSSTQPKMLAIFWFHAHEI-QYHACLIQVFFIH 187
>gi|169173146 PREDICTED: hypothetical protein [Homo sapiens]
Length = 464
Score = 29.6 bits (65), Expect = 4.9
Identities = 13/30 (43%), Positives = 19/30 (63%)
Query: 40 RGIPISELKNHGILQALTTEAYEWEPRVVS 69
R +P + K HG+LQA+ T+A + P V S
Sbjct: 238 REVPQAASKTHGLLQAVRTQAQDKRPAVTS 267
>gi|113419909 PREDICTED: hypothetical protein [Homo sapiens]
Length = 464
Score = 29.6 bits (65), Expect = 4.9
Identities = 13/30 (43%), Positives = 19/30 (63%)
Query: 40 RGIPISELKNHGILQALTTEAYEWEPRVVS 69
R +P + K HG+LQA+ T+A + P V S
Sbjct: 238 REVPQAASKTHGLLQAVRTQAQDKRPAVTS 267
>gi|23592220 G protein-coupled receptor 26 [Homo sapiens]
Length = 337
Score = 28.9 bits (63), Expect = 8.3
Identities = 21/90 (23%), Positives = 41/90 (45%), Gaps = 3/90 (3%)
Query: 206 PATDLRGAGFLALLHLLYLVMDSKTLPMAQ-EIFRLSRHHIQQFPFCLMSVNITHIAIQ- 263
P LR A F H L ++ L ++ +++R H ++ M + + +
Sbjct: 163 PDERLRFAVFTGAFHALSFLLSFVVLCCTYLKVLKVARFHCKRIDVITMQTLVLLVDLHP 222
Query: 264 ALREECLSRECNRQQKVIPVVNSFYAATFL 293
++RE CL + R+Q+ +++F TFL
Sbjct: 223 SVRERCLEEQKRRRQRATKKISTF-IGTFL 251
Database: hs.faa
Posted date: Aug 4, 2009 4:42 PM
Number of letters in database: 18,247,518
Number of sequences in database: 37,866
Lambda K H
0.322 0.136 0.410
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,980,172
Number of Sequences: 37866
Number of extensions: 639489
Number of successful extensions: 1469
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 1457
Number of HSP's gapped (non-prelim): 15
length of query: 391
length of database: 18,247,518
effective HSP length: 104
effective length of query: 287
effective length of database: 14,309,454
effective search space: 4106813298
effective search space used: 4106813298
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 63 (28.9 bits)
Search results were obtained with NCBI BLAST and RefSeq entries.
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Guide to the Human Genome
Copyright © 2010 by Stewart Scherer. All rights reserved.