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Search of human proteins with 4503945
BLASTP 2.2.11 [Jun-05-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= gi|4503945 glucagon preproprotein [Homo sapiens]
(180 letters)
Database: hs.faa
37,866 sequences; 18,247,518 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|4503945 glucagon preproprotein [Homo sapiens] 365 e-101
gi|4507897 vasoactive intestinal peptide isoform 1 preproprotein... 53 1e-07
gi|37588853 vasoactive intestinal peptide isoform 2 preproprotei... 50 1e-06
gi|4758436 gastric inhibitory polypeptide preproprotein [Homo sa... 45 4e-05
gi|211064487 hypothetical protein LOC84886 isoform 3 [Homo sapiens] 33 0.17
gi|211063485 hypothetical protein LOC84886 isoform 2 [Homo sapiens] 33 0.17
gi|14249478 hypothetical protein LOC84886 isoform 1 [Homo sapiens] 33 0.17
gi|11345450 secretin preproprotein [Homo sapiens] 32 0.29
gi|153266792 adenylate cyclase activating polypeptide precursor ... 30 0.86
gi|153266795 adenylate cyclase activating polypeptide precursor ... 30 0.86
gi|59859878 baculoviral IAP repeat-containing protein 5 isoform ... 29 1.9
gi|116089337 IQ motif containing GTPase activating protein 2 [Ho... 29 2.5
gi|167234419 thyroid hormone receptor associated protein 3 [Homo... 28 3.3
gi|50409738 SRp25 nuclear protein isoform 3 [Homo sapiens] 27 7.3
gi|226371754 katanin p60 subunit A-like 2 [Homo sapiens] 27 9.5
gi|58530886 interleukin-1 receptor-associated kinase 2 [Homo sap... 27 9.5
gi|34850061 superiorcervical ganglia, neural specific 10 [Homo s... 27 9.5
gi|71274144 AT hook, DNA binding motif, containing 1 [Homo sapiens] 27 9.5
>gi|4503945 glucagon preproprotein [Homo sapiens]
Length = 180
Score = 365 bits (936), Expect = e-101
Identities = 180/180 (100%), Positives = 180/180 (100%)
Query: 1 MKSIYFVAGLFVMLVQGSWQRSLQDTEEKSRSFSASQADPLSDPDQMNEDKRHSQGTFTS 60
MKSIYFVAGLFVMLVQGSWQRSLQDTEEKSRSFSASQADPLSDPDQMNEDKRHSQGTFTS
Sbjct: 1 MKSIYFVAGLFVMLVQGSWQRSLQDTEEKSRSFSASQADPLSDPDQMNEDKRHSQGTFTS 60
Query: 61 DYSKYLDSRRAQDFVQWLMNTKRNRNNIAKRHDEFERHAEGTFTSDVSSYLEGQAAKEFI 120
DYSKYLDSRRAQDFVQWLMNTKRNRNNIAKRHDEFERHAEGTFTSDVSSYLEGQAAKEFI
Sbjct: 61 DYSKYLDSRRAQDFVQWLMNTKRNRNNIAKRHDEFERHAEGTFTSDVSSYLEGQAAKEFI 120
Query: 121 AWLVKGRGRRDFPEEVAIVEELGRRHADGSFSDEMNTILDNLAARDFINWLIQTKITDRK 180
AWLVKGRGRRDFPEEVAIVEELGRRHADGSFSDEMNTILDNLAARDFINWLIQTKITDRK
Sbjct: 121 AWLVKGRGRRDFPEEVAIVEELGRRHADGSFSDEMNTILDNLAARDFINWLIQTKITDRK 180
>gi|4507897 vasoactive intestinal peptide isoform 1 preproprotein
[Homo sapiens]
Length = 170
Score = 53.1 bits (126), Expect = 1e-07
Identities = 35/122 (28%), Positives = 65/122 (53%), Gaps = 9/122 (7%)
Query: 20 QRSLQDTEEKSRSFSASQADPLSDPDQMNEDKRHSQGTFTSDYSKYLDSRRAQDFVQWLM 79
Q SL++ + ++ A P D ++ + RH+ G FTSD+SK L A+ +++ LM
Sbjct: 51 QVSLKEDIDMLQNALAENDTPYYD---VSRNARHADGVFTSDFSKLLGQLSAKKYLESLM 107
Query: 80 NTKRNRNNIAKRHDEFERHAEGTFTSDVSSYLEGQAAKEFIAWLVKGRGRR-----DFPE 134
KR +NI++ +RH++ FT + + + A K+++ ++ G+ DFPE
Sbjct: 108 G-KRVSSNISEDPVPVKRHSDAVFTDNYTRLRKQMAVKKYLNSILNGKRSSEGESPDFPE 166
Query: 135 EV 136
E+
Sbjct: 167 EL 168
Score = 43.9 bits (102), Expect = 7e-05
Identities = 24/79 (30%), Positives = 42/79 (53%), Gaps = 4/79 (5%)
Query: 97 RHAEGTFTSDVSSYLEGQAAKEFIAWLVKGRGRRDFPEEVAIVEELGRRHADGSFSDEMN 156
RHA+G FTSD S L +AK+++ L+ R + E+ V +RH+D F+D
Sbjct: 80 RHADGVFTSDFSKLLGQLSAKKYLESLMGKRVSSNISEDPVPV----KRHSDAVFTDNYT 135
Query: 157 TILDNLAARDFINWLIQTK 175
+ +A + ++N ++ K
Sbjct: 136 RLRKQMAVKKYLNSILNGK 154
Score = 28.1 bits (61), Expect = 4.3
Identities = 10/33 (30%), Positives = 22/33 (66%)
Query: 145 RHADGSFSDEMNTILDNLAARDFINWLIQTKIT 177
RHADG F+ + + +L L+A+ ++ L+ +++
Sbjct: 80 RHADGVFTSDFSKLLGQLSAKKYLESLMGKRVS 112
>gi|37588853 vasoactive intestinal peptide isoform 2 preproprotein
[Homo sapiens]
Length = 169
Score = 50.1 bits (118), Expect = 1e-06
Identities = 33/122 (27%), Positives = 64/122 (52%), Gaps = 10/122 (8%)
Query: 20 QRSLQDTEEKSRSFSASQADPLSDPDQMNEDKRHSQGTFTSDYSKYLDSRRAQDFVQWLM 79
Q SL++ + ++ A P D ++ + RH+ G FTSD+SK L A+ +++ LM
Sbjct: 51 QVSLKEDIDMLQNALAENDTPYYD---VSRNARHADGVFTSDFSKLLGQLSAKKYLESLM 107
Query: 80 NTKRNRNNIAKRHDEFERHAEGTFTSDVSSYLEGQAAKEFIAWLVKGRGRR-----DFPE 134
+ +NI++ +RH++ FT + + + A K+++ ++ G+ DFPE
Sbjct: 108 G--KRVSNISEDPVPVKRHSDAVFTDNYTRLRKQMAVKKYLNSILNGKRSSEGESPDFPE 165
Query: 135 EV 136
E+
Sbjct: 166 EL 167
Score = 42.0 bits (97), Expect = 3e-04
Identities = 23/79 (29%), Positives = 41/79 (51%), Gaps = 5/79 (6%)
Query: 97 RHAEGTFTSDVSSYLEGQAAKEFIAWLVKGRGRRDFPEEVAIVEELGRRHADGSFSDEMN 156
RHA+G FTSD S L +AK+++ L+ R + V + +RH+D F+D
Sbjct: 80 RHADGVFTSDFSKLLGQLSAKKYLESLMGKRVSNISEDPVPV-----KRHSDAVFTDNYT 134
Query: 157 TILDNLAARDFINWLIQTK 175
+ +A + ++N ++ K
Sbjct: 135 RLRKQMAVKKYLNSILNGK 153
Score = 28.5 bits (62), Expect = 3.3
Identities = 10/34 (29%), Positives = 23/34 (67%)
Query: 145 RHADGSFSDEMNTILDNLAARDFINWLIQTKITD 178
RHADG F+ + + +L L+A+ ++ L+ ++++
Sbjct: 80 RHADGVFTSDFSKLLGQLSAKKYLESLMGKRVSN 113
>gi|4758436 gastric inhibitory polypeptide preproprotein [Homo
sapiens]
Length = 153
Score = 44.7 bits (104), Expect = 4e-05
Identities = 28/95 (29%), Positives = 50/95 (52%), Gaps = 6/95 (6%)
Query: 27 EEKSRSFSASQADPLSDPDQMNEDK----RHSQGTFTSDYSKYLDSRRAQDFVQWLMNTK 82
E+K FSA + P+ +++ + R+++GTF SDYS +D QDFV WL+ K
Sbjct: 22 EKKEGHFSALPSLPVGSHAKVSSPQPRGPRYAEGTFISDYSIAMDKIHQQDFVNWLLAQK 81
Query: 83 RNRNNIAKRHDEFERHAEGTFTSDVSSYLEGQAAK 117
+N+ +H+ +R A + ++ E +A +
Sbjct: 82 GKKND--WKHNITQREARALELASQANRKEEEAVE 114
Score = 34.7 bits (78), Expect = 0.045
Identities = 13/41 (31%), Positives = 26/41 (63%)
Query: 135 EVAIVEELGRRHADGSFSDEMNTILDNLAARDFINWLIQTK 175
+V+ + G R+A+G+F + + +D + +DF+NWL+ K
Sbjct: 41 KVSSPQPRGPRYAEGTFISDYSIAMDKIHQQDFVNWLLAQK 81
Score = 32.7 bits (73), Expect = 0.17
Identities = 13/35 (37%), Positives = 24/35 (68%)
Query: 97 RHAEGTFTSDVSSYLEGQAAKEFIAWLVKGRGRRD 131
R+AEGTF SD S ++ ++F+ WL+ +G+++
Sbjct: 51 RYAEGTFISDYSIAMDKIHQQDFVNWLLAQKGKKN 85
>gi|211064487 hypothetical protein LOC84886 isoform 3 [Homo
sapiens]
Length = 197
Score = 32.7 bits (73), Expect = 0.17
Identities = 17/59 (28%), Positives = 32/59 (54%)
Query: 27 EEKSRSFSASQADPLSDPDQMNEDKRHSQGTFTSDYSKYLDSRRAQDFVQWLMNTKRNR 85
+E S +AS+ PLS Q ++ + SQG+ +S +R+ ++ W +N +R+R
Sbjct: 11 QEPSNGTAASEPRPLSKASQGSQALKSSQGSRSSSLDALGPTRKEEEASFWKINAERSR 69
>gi|211063485 hypothetical protein LOC84886 isoform 2 [Homo sapiens]
Length = 289
Score = 32.7 bits (73), Expect = 0.17
Identities = 17/59 (28%), Positives = 32/59 (54%)
Query: 27 EEKSRSFSASQADPLSDPDQMNEDKRHSQGTFTSDYSKYLDSRRAQDFVQWLMNTKRNR 85
+E S +AS+ PLS Q ++ + SQG+ +S +R+ ++ W +N +R+R
Sbjct: 103 QEPSNGTAASEPRPLSKASQGSQALKSSQGSRSSSLDALGPTRKEEEASFWKINAERSR 161
>gi|14249478 hypothetical protein LOC84886 isoform 1 [Homo sapiens]
Length = 327
Score = 32.7 bits (73), Expect = 0.17
Identities = 17/59 (28%), Positives = 32/59 (54%)
Query: 27 EEKSRSFSASQADPLSDPDQMNEDKRHSQGTFTSDYSKYLDSRRAQDFVQWLMNTKRNR 85
+E S +AS+ PLS Q ++ + SQG+ +S +R+ ++ W +N +R+R
Sbjct: 141 QEPSNGTAASEPRPLSKASQGSQALKSSQGSRSSSLDALGPTRKEEEASFWKINAERSR 199
>gi|11345450 secretin preproprotein [Homo sapiens]
Length = 121
Score = 32.0 bits (71), Expect = 0.29
Identities = 15/41 (36%), Positives = 23/41 (56%)
Query: 97 RHAEGTFTSDVSSYLEGQAAKEFIAWLVKGRGRRDFPEEVA 137
RH++GTFTS++S EG + + LV R +D +A
Sbjct: 27 RHSDGTFTSELSRLREGARLQRLLQGLVGKRSEQDAENSMA 67
Score = 30.8 bits (68), Expect = 0.66
Identities = 13/36 (36%), Positives = 22/36 (61%)
Query: 51 KRHSQGTFTSDYSKYLDSRRAQDFVQWLMNTKRNRN 86
+RHS GTFTS+ S+ + R Q +Q L+ + ++
Sbjct: 26 RRHSDGTFTSELSRLREGARLQRLLQGLVGKRSEQD 61
>gi|153266792 adenylate cyclase activating polypeptide precursor
[Homo sapiens]
Length = 176
Score = 30.4 bits (67), Expect = 0.86
Identities = 25/89 (28%), Positives = 38/89 (42%), Gaps = 12/89 (13%)
Query: 54 SQGTFTSDYSKYLDSRRAQDFVQWLMNTKRNRNNIAKRHDEFE----RHAEGTFTSDVSS 109
+ G Y K LD A +Q L+ + D+ E RH++G FT S
Sbjct: 84 AHGILNEAYRKVLDQLSAGKHLQSLVARGVGGSLGGGAGDDAEPLSKRHSDGIFTDSYSR 143
Query: 110 YLEGQAAKEFIAWL--------VKGRGRR 130
Y + A K+++A + VK +GRR
Sbjct: 144 YRKQMAVKKYLAAVLGKRYKQRVKNKGRR 172
Score = 28.1 bits (61), Expect = 4.3
Identities = 14/32 (43%), Positives = 17/32 (53%), Gaps = 8/32 (25%)
Query: 34 SASQADPLSDPDQMNEDKRHSQGTFTSDYSKY 65
+ A+PLS KRHS G FT YS+Y
Sbjct: 121 AGDDAEPLS--------KRHSDGIFTDSYSRY 144
>gi|153266795 adenylate cyclase activating polypeptide precursor
[Homo sapiens]
Length = 176
Score = 30.4 bits (67), Expect = 0.86
Identities = 25/89 (28%), Positives = 38/89 (42%), Gaps = 12/89 (13%)
Query: 54 SQGTFTSDYSKYLDSRRAQDFVQWLMNTKRNRNNIAKRHDEFE----RHAEGTFTSDVSS 109
+ G Y K LD A +Q L+ + D+ E RH++G FT S
Sbjct: 84 AHGILNEAYRKVLDQLSAGKHLQSLVARGVGGSLGGGAGDDAEPLSKRHSDGIFTDSYSR 143
Query: 110 YLEGQAAKEFIAWL--------VKGRGRR 130
Y + A K+++A + VK +GRR
Sbjct: 144 YRKQMAVKKYLAAVLGKRYKQRVKNKGRR 172
Score = 28.1 bits (61), Expect = 4.3
Identities = 14/32 (43%), Positives = 17/32 (53%), Gaps = 8/32 (25%)
Query: 34 SASQADPLSDPDQMNEDKRHSQGTFTSDYSKY 65
+ A+PLS KRHS G FT YS+Y
Sbjct: 121 AGDDAEPLS--------KRHSDGIFTDSYSRY 144
>gi|59859878 baculoviral IAP repeat-containing protein 5 isoform 1
[Homo sapiens]
Length = 142
Score = 29.3 bits (64), Expect = 1.9
Identities = 19/62 (30%), Positives = 30/62 (48%), Gaps = 6/62 (9%)
Query: 43 DPDQMNEDKRHSQGTFTSDYSKYLDSRRAQDFVQWLMNTKRNRNNIAK----RHDEFERH 98
D D + E K+HS G K + +F++ ++ +R +N IAK + EFE
Sbjct: 70 DDDPIEEHKKHSSGCAFLSVKKQFEELTLGEFLK--LDRERAKNKIAKETNNKKKEFEET 127
Query: 99 AE 100
AE
Sbjct: 128 AE 129
>gi|116089337 IQ motif containing GTPase activating protein 2 [Homo
sapiens]
Length = 1575
Score = 28.9 bits (63), Expect = 2.5
Identities = 15/68 (22%), Positives = 32/68 (47%)
Query: 21 RSLQDTEEKSRSFSASQADPLSDPDQMNEDKRHSQGTFTSDYSKYLDSRRAQDFVQWLMN 80
+ L D+E S+ + D ++ED+ T D ++ L+ +++ D + L +
Sbjct: 504 QKLGDSESVSKVLWLDEIQQAVDDANVDEDRAKQWVTLVVDVNQCLEGKKSSDILSVLKS 563
Query: 81 TKRNRNNI 88
+ N N+I
Sbjct: 564 STSNANDI 571
>gi|167234419 thyroid hormone receptor associated protein 3 [Homo
sapiens]
Length = 955
Score = 28.5 bits (62), Expect = 3.3
Identities = 29/114 (25%), Positives = 46/114 (40%), Gaps = 9/114 (7%)
Query: 24 QDTEEKSRSFSASQADPLSDPDQMNEDKR---HSQGTFTSDYSKYLDSRRAQDFVQWLMN 80
++TEE+ S + L D + +R ++GTF ++ R + N
Sbjct: 797 EETEEREESTTGFDKSRLGTKDFVGPSERGGGRARGTF-----QFRARGRGWGRGNYSGN 851
Query: 81 TKRNRNN-IAKRHDEFERHAEGTFTSDVSSYLEGQAAKEFIAWLVKGRGRRDFP 133
N NN KR+ E E E T S + + + W+ +GRGR FP
Sbjct: 852 NNNNSNNDFQKRNREEEWDPEYTPKSKKYYLHDDREGEGSDKWVSRGRGRGAFP 905
>gi|50409738 SRp25 nuclear protein isoform 3 [Homo sapiens]
Length = 352
Score = 27.3 bits (59), Expect = 7.3
Identities = 19/78 (24%), Positives = 30/78 (38%), Gaps = 9/78 (11%)
Query: 17 GSWQRSLQDTEEKSRSFSASQADPLSDPDQMNEDKRHSQGTFTSDYSKYLDSRRAQDFVQ 76
G+ +RS Q ++RS S+S + S + S KY D RR +
Sbjct: 174 GAEERSKQKARRRTRSSSSSSSSSSSSSSSSSSSSSSSSSDGRKKRGKYKDKRRKK---- 229
Query: 77 WLMNTKRNRNNIAKRHDE 94
K+ R + K+ E
Sbjct: 230 -----KKKRKKLKKKGKE 242
>gi|226371754 katanin p60 subunit A-like 2 [Homo sapiens]
Length = 466
Score = 26.9 bits (58), Expect = 9.5
Identities = 12/35 (34%), Positives = 21/35 (60%)
Query: 66 LDSRRAQDFVQWLMNTKRNRNNIAKRHDEFERHAE 100
LD DF+ L +TK + N+A+R+ +++R E
Sbjct: 430 LDIVTTADFLDVLTHTKPSAKNLAQRYSDWQREFE 464
>gi|58530886 interleukin-1 receptor-associated kinase 2 [Homo
sapiens]
Length = 625
Score = 26.9 bits (58), Expect = 9.5
Identities = 11/27 (40%), Positives = 17/27 (62%)
Query: 36 SQADPLSDPDQMNEDKRHSQGTFTSDY 62
S+AD + D N++++ SQGTF Y
Sbjct: 199 SEADVVQATDDFNQNRKISQGTFADVY 225
>gi|34850061 superiorcervical ganglia, neural specific 10 [Homo
sapiens]
Length = 179
Score = 26.9 bits (58), Expect = 9.5
Identities = 19/80 (23%), Positives = 34/80 (42%)
Query: 20 QRSLQDTEEKSRSFSASQADPLSDPDQMNEDKRHSQGTFTSDYSKYLDSRRAQDFVQWLM 79
Q+ L+ EE+ +S A L++ + + +++SK + + Q
Sbjct: 85 QKKLEAAEERRKSQEAQVLKQLAEKREHEREVLQKALEENNNFSKMAEEKLILKMEQIKE 144
Query: 80 NTKRNRNNIAKRHDEFERHA 99
N + N I +R E ERHA
Sbjct: 145 NREANLAAIIERLQEKERHA 164
>gi|71274144 AT hook, DNA binding motif, containing 1 [Homo sapiens]
Length = 1603
Score = 26.9 bits (58), Expect = 9.5
Identities = 22/99 (22%), Positives = 36/99 (36%), Gaps = 10/99 (10%)
Query: 16 QGSWQRSLQDTEEKSRSFSASQADPLSDPDQMNEDKRHSQGTFTSDYSKYLDSRRAQDFV 75
QG+ R+ T +S + P E + Q FT + LDS + D +
Sbjct: 794 QGTEARAFASTGLESGASGRGSYYSTGAPSGQTELSQERQNLFTGYFRSLLDSDDSSDLL 853
Query: 76 QWLMNTKRNRNNIAKRHDEFERHAEGTFTSDVSSYLEGQ 114
+ ++ R + R A GT+ +S L Q
Sbjct: 854 DFALSASRPES----------RKASGTYAGPPTSALPAQ 882
Database: hs.faa
Posted date: Aug 4, 2009 4:42 PM
Number of letters in database: 18,247,518
Number of sequences in database: 37,866
Lambda K H
0.317 0.131 0.381
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,311,254
Number of Sequences: 37866
Number of extensions: 250659
Number of successful extensions: 770
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 14
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 732
Number of HSP's gapped (non-prelim): 30
length of query: 180
length of database: 18,247,518
effective HSP length: 96
effective length of query: 84
effective length of database: 14,612,382
effective search space: 1227440088
effective search space used: 1227440088
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
Search results were obtained with NCBI BLAST and RefSeq entries.
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Guide to the Human Genome
Copyright © 2010 by Stewart Scherer. All rights reserved.