Search of human proteins with 4502499

Search of human proteins with 4502499

BLASTP 2.2.11 [Jun-05-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|4502499 cholesterol 25-hydroxylase [Homo sapiens]
         (272 letters)

Database: hs.faa 
           37,866 sequences; 18,247,518 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|4502499 cholesterol 25-hydroxylase [Homo sapiens]                  595   e-170
gi|5803157 sterol-C4-methyl oxidase-like isoform 1 [Homo sapiens]     107   1e-23
gi|62865628 sterol-C4-methyl oxidase-like isoform 2 [Homo sapiens]     92   4e-19
gi|68160945 sterol-C5-desaturase [Homo sapiens]                        51   9e-07
gi|68160941 sterol-C5-desaturase [Homo sapiens]                        51   9e-07
gi|146198575 hypothetical protein LOC10826 isoform 2 [Homo sapiens]    44   2e-04
gi|169215606 PREDICTED: similar to immunoglobulin superfamily, m...    30   1.7  
gi|31442761 amiloride-sensitive cation channel 5, intestinal [Ho...    30   1.7  
gi|239746626 PREDICTED: similar to KIAA0466 protein [Homo sapiens]     30   2.9  

>gi|4502499 cholesterol 25-hydroxylase [Homo sapiens]
          Length = 272

 Score =  595 bits (1535), Expect = e-170
 Identities = 272/272 (100%), Positives = 272/272 (100%)

Query: 1   MSCHNCSDPQVLCSSGQLFLQPLWDHLRSWEALLQSPFFPVIFSITTYVGFCLPFVVLDI 60
           MSCHNCSDPQVLCSSGQLFLQPLWDHLRSWEALLQSPFFPVIFSITTYVGFCLPFVVLDI
Sbjct: 1   MSCHNCSDPQVLCSSGQLFLQPLWDHLRSWEALLQSPFFPVIFSITTYVGFCLPFVVLDI 60

Query: 61  LCSWVPALRRYKIHPDFSPSAQQLLPCLGQTLYQHVMFVFPVTLLHWARSPALLPHEAPE 120
           LCSWVPALRRYKIHPDFSPSAQQLLPCLGQTLYQHVMFVFPVTLLHWARSPALLPHEAPE
Sbjct: 61  LCSWVPALRRYKIHPDFSPSAQQLLPCLGQTLYQHVMFVFPVTLLHWARSPALLPHEAPE 120

Query: 121 LLLLLHHILFCLLLFDMEFFVWHLLHHKVPWLYRTFHKVHHQNSSSFALATQYMSVWELF 180
           LLLLLHHILFCLLLFDMEFFVWHLLHHKVPWLYRTFHKVHHQNSSSFALATQYMSVWELF
Sbjct: 121 LLLLLHHILFCLLLFDMEFFVWHLLHHKVPWLYRTFHKVHHQNSSSFALATQYMSVWELF 180

Query: 181 SLGFFDMMNVTLLGCHPLTTLTFHVVNIWLSVEDHSGYNFPWSTHRLVPFGWYGGVVHHD 240
           SLGFFDMMNVTLLGCHPLTTLTFHVVNIWLSVEDHSGYNFPWSTHRLVPFGWYGGVVHHD
Sbjct: 181 SLGFFDMMNVTLLGCHPLTTLTFHVVNIWLSVEDHSGYNFPWSTHRLVPFGWYGGVVHHD 240

Query: 241 LHHSHFNCNFAPYFTHWDKILGTLRTASVPAR 272
           LHHSHFNCNFAPYFTHWDKILGTLRTASVPAR
Sbjct: 241 LHHSHFNCNFAPYFTHWDKILGTLRTASVPAR 272


>gi|5803157 sterol-C4-methyl oxidase-like isoform 1 [Homo sapiens]
          Length = 293

 Score =  107 bits (267), Expect = 1e-23
 Identities = 66/221 (29%), Positives = 105/221 (47%), Gaps = 13/221 (5%)

Query: 48  YVGFCLPFVVLDILCSWVPALRRYKIHPDFSPSAQQLLPCLGQTLYQHVMFVFPVTL-LH 106
           Y  FCLP      L  ++P +++YKI  D   + +    C    L+ H     P+    +
Sbjct: 62  YFLFCLP----GFLFQFIPYMKKYKIQKDKPETWENQWKCFKVLLFNHFCIQLPLICGTY 117

Query: 107 WARSPALLPHE---APELLLLLHHILFCLLLFDM-EFFVWHLLHHKVPWLYRTFHKVHHQ 162
           +      +P++    P    LL     C ++ D   +F+  LLHHK   +Y+  HKVHH+
Sbjct: 118 YFTEYFNIPYDWERMPRWYFLLARCFGCAVIEDTWHYFLHRLLHHKR--IYKYIHKVHHE 175

Query: 163 NSSSFALATQYMSVWELFSLGFFDMMNVTLLGCHPLTTLTFHVVNIWLSVEDHSGYNFPW 222
             + F +  +Y    E   LG    + + LL  H +    +  + +  +++ HSGY+ P 
Sbjct: 176 FQAPFGMEAEYAHPLETLILGTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPL 235

Query: 223 STHRLVPFGWYGGVVHHDLHHSHFNCNFAPYFTHWDKILGT 263
           +   L+PF  Y G  HHD HH +F  N+A  FT WD+I GT
Sbjct: 236 NPLNLIPF--YAGSRHHDFHHMNFIGNYASTFTWWDRIFGT 274


>gi|62865628 sterol-C4-methyl oxidase-like isoform 2 [Homo sapiens]
          Length = 162

 Score = 92.4 bits (228), Expect = 4e-19
 Identities = 50/146 (34%), Positives = 75/146 (51%), Gaps = 5/146 (3%)

Query: 119 PELLLLLHHILFCLLLFDM-EFFVWHLLHHKVPWLYRTFHKVHHQNSSSFALATQYMSVW 177
           P    LL     C ++ D   +F+  LLHHK   +Y+  HKVHH+  + F +  +Y    
Sbjct: 2   PRWYFLLARCFGCAVIEDTWHYFLHRLLHHKR--IYKYIHKVHHEFQAPFGMEAEYAHPL 59

Query: 178 ELFSLGFFDMMNVTLLGCHPLTTLTFHVVNIWLSVEDHSGYNFPWSTHRLVPFGWYGGVV 237
           E   LG    + + LL  H +    +  + +  +++ HSGY+ P +   L+PF  Y G  
Sbjct: 60  ETLILGTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNLIPF--YAGSR 117

Query: 238 HHDLHHSHFNCNFAPYFTHWDKILGT 263
           HHD HH +F  N+A  FT WD+I GT
Sbjct: 118 HHDFHHMNFIGNYASTFTWWDRIFGT 143


>gi|68160945 sterol-C5-desaturase [Homo sapiens]
          Length = 299

 Score = 51.2 bits (121), Expect = 9e-07
 Identities = 40/140 (28%), Positives = 64/140 (45%), Gaps = 13/140 (9%)

Query: 134 LFDMEFFVWHL---LHHKVPWLYRTFHKVHHQNSSSFALATQYMSVWELFSLGFFDMMNV 190
           LF  + F++ +   LHH++  +Y+  HK HH        A+      + F       +  
Sbjct: 127 LFFTDMFIYWIHRGLHHRL--VYKRLHKPHHIWKIPTPFASHAFHPIDGFLQSLPYHIYP 184

Query: 191 TLLGCHPLTTLTFHV-VNIWLSVEDHSG-YNFPWSTHRLVPFGWYGGVVHHDLHHSHFNC 248
            +   H +  L+ ++ VNIW ++  H G +  P     L PF    G  HH  HH  F+ 
Sbjct: 185 FIFPLHKVVYLSLYILVNIW-TISIHDGDFRVP---QILQPF--INGSAHHTDHHMFFDY 238

Query: 249 NFAPYFTHWDKILGTLRTAS 268
           N+  YFT WD+I G+ +  S
Sbjct: 239 NYGQYFTLWDRIGGSFKNPS 258


>gi|68160941 sterol-C5-desaturase [Homo sapiens]
          Length = 299

 Score = 51.2 bits (121), Expect = 9e-07
 Identities = 40/140 (28%), Positives = 64/140 (45%), Gaps = 13/140 (9%)

Query: 134 LFDMEFFVWHL---LHHKVPWLYRTFHKVHHQNSSSFALATQYMSVWELFSLGFFDMMNV 190
           LF  + F++ +   LHH++  +Y+  HK HH        A+      + F       +  
Sbjct: 127 LFFTDMFIYWIHRGLHHRL--VYKRLHKPHHIWKIPTPFASHAFHPIDGFLQSLPYHIYP 184

Query: 191 TLLGCHPLTTLTFHV-VNIWLSVEDHSG-YNFPWSTHRLVPFGWYGGVVHHDLHHSHFNC 248
            +   H +  L+ ++ VNIW ++  H G +  P     L PF    G  HH  HH  F+ 
Sbjct: 185 FIFPLHKVVYLSLYILVNIW-TISIHDGDFRVP---QILQPF--INGSAHHTDHHMFFDY 238

Query: 249 NFAPYFTHWDKILGTLRTAS 268
           N+  YFT WD+I G+ +  S
Sbjct: 239 NYGQYFTLWDRIGGSFKNPS 258


>gi|146198575 hypothetical protein LOC10826 isoform 2 [Homo sapiens]
          Length = 333

 Score = 43.9 bits (102), Expect = 2e-04
 Identities = 39/160 (24%), Positives = 65/160 (40%), Gaps = 16/160 (10%)

Query: 90  QTLYQHVMFVFPVTLLHWARSPALLPHEAPELLLLLHHILFCLLLFDMEFFVWH-LLHHK 148
           Q +    M VF    L W R P     E P     L  +    L+ ++ F+  H LLHH 
Sbjct: 139 QCMISFPMVVFLYPFLKWWRDPCR--RELPTFHWFLLELAIFTLIEEVLFYYSHRLLHH- 195

Query: 149 VPWLYRTFHKVHHQNSSSFALATQYMSVWELFSLGFFD-MMNVTLLGCHPLTTLTFHVVN 207
            P  Y+  HK HH+ ++   + + Y    E         ++   ++G H  +   +  + 
Sbjct: 196 -PTFYKKIHKKHHEWTAPIGVISLYAHPIEHAVSNMLPVIVGPLVMGSHLSSITMWFSLA 254

Query: 208 IWLSVEDHSGYNFPWSTHRLVPFGWYGGVVHHDLHHSHFN 247
           + ++   H GY+ P+     +P   +     HD HH  FN
Sbjct: 255 LIITTISHCGYHLPF-----LPSPEF-----HDYHHLKFN 284


>gi|169215606 PREDICTED: similar to immunoglobulin superfamily,
           member 3 [Homo sapiens]
          Length = 195

 Score = 30.4 bits (67), Expect = 1.7
 Identities = 24/93 (25%), Positives = 37/93 (39%), Gaps = 22/93 (23%)

Query: 136 DMEFFVWHLLHHKVPWLYRTFHKVHHQNSSSFALATQYMSVWELFSLGFFDMMNVTLLGC 195
           D+  F WHL HH+  +L ++      QN+ S   A + ++V E                 
Sbjct: 50  DLAIFQWHLFHHE--FLRQSDGATLFQNNHSVVSAQRQVTVQE----------------- 90

Query: 196 HPLTTLTFHVVNIWLSVEDHSG---YNFPWSTH 225
            PL       + IW +V  + G    NF WS +
Sbjct: 91  GPLYRTEGSHITIWCNVSGYQGPSEQNFQWSIY 123


>gi|31442761 amiloride-sensitive cation channel 5, intestinal [Homo
           sapiens]
          Length = 505

 Score = 30.4 bits (67), Expect = 1.7
 Identities = 17/61 (27%), Positives = 29/61 (47%)

Query: 135 FDMEFFVWHLLHHKVPWLYRTFHKVHHQNSSSFALATQYMSVWELFSLGFFDMMNVTLLG 194
           F + FF+WH++   +     T +    + ++ FA + Q  S+ E      F + N TLL 
Sbjct: 125 FGVIFFLWHIVSKVLHLQEITANSTGSREATDFAASHQNFSIVEFIRNKGFYLNNSTLLD 184

Query: 195 C 195
           C
Sbjct: 185 C 185


>gi|239746626 PREDICTED: similar to KIAA0466 protein [Homo sapiens]
          Length = 155

 Score = 29.6 bits (65), Expect = 2.9
 Identities = 24/93 (25%), Positives = 37/93 (39%), Gaps = 22/93 (23%)

Query: 136 DMEFFVWHLLHHKVPWLYRTFHKVHHQNSSSFALATQYMSVWELFSLGFFDMMNVTLLGC 195
           D+  F WHL HH+  +L ++      QN+ S   A + ++V E                 
Sbjct: 10  DLAIFQWHLFHHE--FLRQSDGATLVQNNHSVVSAQRQVTVQE----------------- 50

Query: 196 HPLTTLTFHVVNIWLSVEDHSG---YNFPWSTH 225
            PL       + IW +V  + G    NF WS +
Sbjct: 51  GPLYRTEGSHITIWCNVSGYQGPSEQNFQWSIY 83


  Database: hs.faa
    Posted date:  Aug 4, 2009  4:42 PM
  Number of letters in database: 18,247,518
  Number of sequences in database:  37,866
  
Lambda     K      H
   0.331    0.142    0.512 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,712,478
Number of Sequences: 37866
Number of extensions: 528318
Number of successful extensions: 1386
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 1377
Number of HSP's gapped (non-prelim): 9
length of query: 272
length of database: 18,247,518
effective HSP length: 101
effective length of query: 171
effective length of database: 14,423,052
effective search space: 2466341892
effective search space used: 2466341892
T: 11
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 61 (28.1 bits)
Search results were obtained with NCBI BLAST and RefSeq entries.