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Search of human proteins with 194328687
BLASTP 2.2.11 [Jun-05-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= gi|194328687 storkhead box 1 isoform b [Homo sapiens]
(227 letters)
Database: hs.faa
37,866 sequences; 18,247,518 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|194328687 storkhead box 1 isoform b [Homo sapiens] 455 e-128
gi|194328691 storkhead box 1 isoform a [Homo sapiens] 443 e-125
gi|63025200 storkhead box 1 isoform a [Homo sapiens] 443 e-125
gi|194328689 storkhead box 1 isoform c [Homo sapiens] 312 1e-85
gi|194328693 storkhead box 1 isoform d [Homo sapiens] 225 3e-59
gi|55742730 storkhead box 2 [Homo sapiens] 129 3e-30
gi|29171705 melanoma antigen family D, 2 [Homo sapiens] 34 0.12
gi|19387846 melanoma antigen family D, 2 [Homo sapiens] 34 0.12
gi|41350320 melanoma antigen family D, 2 [Homo sapiens] 34 0.12
gi|110611172 amnionless protein precursor [Homo sapiens] 32 0.45
gi|239755540 PREDICTED: hypothetical protein [Homo sapiens] 28 4.9
gi|239755457 PREDICTED: similar to patatin-like phospholipase do... 28 4.9
gi|239749992 PREDICTED: hypothetical protein [Homo sapiens] 28 4.9
gi|239744303 PREDICTED: hypothetical protein XP_002343093 [Homo ... 28 4.9
gi|169169855 PREDICTED: similar to hCG34808 [Homo sapiens] 28 6.4
gi|239752201 PREDICTED: hypothetical protein XP_002348147 [Homo ... 28 6.4
gi|169168879 PREDICTED: similar to hCG34808 [Homo sapiens] 28 6.4
gi|169169326 PREDICTED: similar to hCG34808 [Homo sapiens] 28 6.4
gi|32698724 patatin-like phospholipase domain containing 2 [Homo... 28 6.4
gi|215422401 hypothetical protein LOC9811 isoform 2 [Homo sapiens] 28 6.4
gi|7662112 hypothetical protein LOC9811 isoform 1 [Homo sapiens] 28 6.4
gi|31542713 MOCO sulphurase C-terminal domain containing 2 [Homo... 28 6.4
>gi|194328687 storkhead box 1 isoform b [Homo sapiens]
Length = 227
Score = 455 bits (1170), Expect = e-128
Identities = 227/227 (100%), Positives = 227/227 (100%)
Query: 1 MARPVQLAPGSLALVLCRLEAQKAAGAAEEPGGRAVFRAFRRANARCFWNARLARAASRL 60
MARPVQLAPGSLALVLCRLEAQKAAGAAEEPGGRAVFRAFRRANARCFWNARLARAASRL
Sbjct: 1 MARPVQLAPGSLALVLCRLEAQKAAGAAEEPGGRAVFRAFRRANARCFWNARLARAASRL 60
Query: 61 AFQGWLRRGVLLVRAPPACLQVLRDAWRRRALRPPRGFRIRAVGDVFPVQMNPITQSQFV 120
AFQGWLRRGVLLVRAPPACLQVLRDAWRRRALRPPRGFRIRAVGDVFPVQMNPITQSQFV
Sbjct: 61 AFQGWLRRGVLLVRAPPACLQVLRDAWRRRALRPPRGFRIRAVGDVFPVQMNPITQSQFV 120
Query: 121 PLGEVLCCAISDMNTAQIVVTQESLLERLMKHYPGIAIPSEDILYTTLGTLIKERKIYHT 180
PLGEVLCCAISDMNTAQIVVTQESLLERLMKHYPGIAIPSEDILYTTLGTLIKERKIYHT
Sbjct: 121 PLGEVLCCAISDMNTAQIVVTQESLLERLMKHYPGIAIPSEDILYTTLGTLIKERKIYHT 180
Query: 181 GEGYFIVTPQTYFITNTTTQENKRMLPSDESRLMPASMTYLDTESGI 227
GEGYFIVTPQTYFITNTTTQENKRMLPSDESRLMPASMTYLDTESGI
Sbjct: 181 GEGYFIVTPQTYFITNTTTQENKRMLPSDESRLMPASMTYLDTESGI 227
>gi|194328691 storkhead box 1 isoform a [Homo sapiens]
Length = 989
Score = 443 bits (1140), Expect = e-125
Identities = 221/221 (100%), Positives = 221/221 (100%)
Query: 1 MARPVQLAPGSLALVLCRLEAQKAAGAAEEPGGRAVFRAFRRANARCFWNARLARAASRL 60
MARPVQLAPGSLALVLCRLEAQKAAGAAEEPGGRAVFRAFRRANARCFWNARLARAASRL
Sbjct: 1 MARPVQLAPGSLALVLCRLEAQKAAGAAEEPGGRAVFRAFRRANARCFWNARLARAASRL 60
Query: 61 AFQGWLRRGVLLVRAPPACLQVLRDAWRRRALRPPRGFRIRAVGDVFPVQMNPITQSQFV 120
AFQGWLRRGVLLVRAPPACLQVLRDAWRRRALRPPRGFRIRAVGDVFPVQMNPITQSQFV
Sbjct: 61 AFQGWLRRGVLLVRAPPACLQVLRDAWRRRALRPPRGFRIRAVGDVFPVQMNPITQSQFV 120
Query: 121 PLGEVLCCAISDMNTAQIVVTQESLLERLMKHYPGIAIPSEDILYTTLGTLIKERKIYHT 180
PLGEVLCCAISDMNTAQIVVTQESLLERLMKHYPGIAIPSEDILYTTLGTLIKERKIYHT
Sbjct: 121 PLGEVLCCAISDMNTAQIVVTQESLLERLMKHYPGIAIPSEDILYTTLGTLIKERKIYHT 180
Query: 181 GEGYFIVTPQTYFITNTTTQENKRMLPSDESRLMPASMTYL 221
GEGYFIVTPQTYFITNTTTQENKRMLPSDESRLMPASMTYL
Sbjct: 181 GEGYFIVTPQTYFITNTTTQENKRMLPSDESRLMPASMTYL 221
>gi|63025200 storkhead box 1 isoform a [Homo sapiens]
Length = 989
Score = 443 bits (1140), Expect = e-125
Identities = 221/221 (100%), Positives = 221/221 (100%)
Query: 1 MARPVQLAPGSLALVLCRLEAQKAAGAAEEPGGRAVFRAFRRANARCFWNARLARAASRL 60
MARPVQLAPGSLALVLCRLEAQKAAGAAEEPGGRAVFRAFRRANARCFWNARLARAASRL
Sbjct: 1 MARPVQLAPGSLALVLCRLEAQKAAGAAEEPGGRAVFRAFRRANARCFWNARLARAASRL 60
Query: 61 AFQGWLRRGVLLVRAPPACLQVLRDAWRRRALRPPRGFRIRAVGDVFPVQMNPITQSQFV 120
AFQGWLRRGVLLVRAPPACLQVLRDAWRRRALRPPRGFRIRAVGDVFPVQMNPITQSQFV
Sbjct: 61 AFQGWLRRGVLLVRAPPACLQVLRDAWRRRALRPPRGFRIRAVGDVFPVQMNPITQSQFV 120
Query: 121 PLGEVLCCAISDMNTAQIVVTQESLLERLMKHYPGIAIPSEDILYTTLGTLIKERKIYHT 180
PLGEVLCCAISDMNTAQIVVTQESLLERLMKHYPGIAIPSEDILYTTLGTLIKERKIYHT
Sbjct: 121 PLGEVLCCAISDMNTAQIVVTQESLLERLMKHYPGIAIPSEDILYTTLGTLIKERKIYHT 180
Query: 181 GEGYFIVTPQTYFITNTTTQENKRMLPSDESRLMPASMTYL 221
GEGYFIVTPQTYFITNTTTQENKRMLPSDESRLMPASMTYL
Sbjct: 181 GEGYFIVTPQTYFITNTTTQENKRMLPSDESRLMPASMTYL 221
>gi|194328689 storkhead box 1 isoform c [Homo sapiens]
Length = 169
Score = 312 bits (800), Expect = 1e-85
Identities = 155/155 (100%), Positives = 155/155 (100%)
Query: 1 MARPVQLAPGSLALVLCRLEAQKAAGAAEEPGGRAVFRAFRRANARCFWNARLARAASRL 60
MARPVQLAPGSLALVLCRLEAQKAAGAAEEPGGRAVFRAFRRANARCFWNARLARAASRL
Sbjct: 1 MARPVQLAPGSLALVLCRLEAQKAAGAAEEPGGRAVFRAFRRANARCFWNARLARAASRL 60
Query: 61 AFQGWLRRGVLLVRAPPACLQVLRDAWRRRALRPPRGFRIRAVGDVFPVQMNPITQSQFV 120
AFQGWLRRGVLLVRAPPACLQVLRDAWRRRALRPPRGFRIRAVGDVFPVQMNPITQSQFV
Sbjct: 61 AFQGWLRRGVLLVRAPPACLQVLRDAWRRRALRPPRGFRIRAVGDVFPVQMNPITQSQFV 120
Query: 121 PLGEVLCCAISDMNTAQIVVTQESLLERLMKHYPG 155
PLGEVLCCAISDMNTAQIVVTQESLLERLMKHYPG
Sbjct: 121 PLGEVLCCAISDMNTAQIVVTQESLLERLMKHYPG 155
>gi|194328693 storkhead box 1 isoform d [Homo sapiens]
Length = 879
Score = 225 bits (573), Expect = 3e-59
Identities = 111/111 (100%), Positives = 111/111 (100%)
Query: 111 MNPITQSQFVPLGEVLCCAISDMNTAQIVVTQESLLERLMKHYPGIAIPSEDILYTTLGT 170
MNPITQSQFVPLGEVLCCAISDMNTAQIVVTQESLLERLMKHYPGIAIPSEDILYTTLGT
Sbjct: 1 MNPITQSQFVPLGEVLCCAISDMNTAQIVVTQESLLERLMKHYPGIAIPSEDILYTTLGT 60
Query: 171 LIKERKIYHTGEGYFIVTPQTYFITNTTTQENKRMLPSDESRLMPASMTYL 221
LIKERKIYHTGEGYFIVTPQTYFITNTTTQENKRMLPSDESRLMPASMTYL
Sbjct: 61 LIKERKIYHTGEGYFIVTPQTYFITNTTTQENKRMLPSDESRLMPASMTYL 111
>gi|55742730 storkhead box 2 [Homo sapiens]
Length = 926
Score = 129 bits (323), Expect = 3e-30
Identities = 62/107 (57%), Positives = 81/107 (75%)
Query: 104 GDVFPVQMNPITQSQFVPLGEVLCCAISDMNTAQIVVTQESLLERLMKHYPGIAIPSEDI 163
GDV P+ M+PI+QSQF+PLGE+LC AIS MN+A+ VTQE+L+E L +PG+ PS++I
Sbjct: 56 GDVSPISMSPISQSQFIPLGEILCLAISAMNSARKPVTQEALMEHLTTCFPGVPTPSQEI 115
Query: 164 LYTTLGTLIKERKIYHTGEGYFIVTPQTYFITNTTTQENKRMLPSDE 210
L TL TL++ERKIY T +GYFIVTPQTYFIT + + N + DE
Sbjct: 116 LRHTLNTLVRERKIYPTPDGYFIVTPQTYFITPSLIRTNSKWYHLDE 162
>gi|29171705 melanoma antigen family D, 2 [Homo sapiens]
Length = 606
Score = 33.9 bits (76), Expect = 0.12
Identities = 27/74 (36%), Positives = 34/74 (45%), Gaps = 15/74 (20%)
Query: 27 AAEEPGGRAVFRAFRRANAR-------CFWNARLARAASRLAFQGWLRRGVLLVRAPPAC 79
A+ GGR V +A + AR FW AR ASR W RR +L +R+P A
Sbjct: 196 ASGTTGGRRVSKALMASMARRASRGPIAFW----ARRASRTRLAAWARRALLSLRSPKA- 250
Query: 80 LQVLRDAWRRRALR 93
R RRRA +
Sbjct: 251 ---RRGKARRRAAK 261
>gi|19387846 melanoma antigen family D, 2 [Homo sapiens]
Length = 606
Score = 33.9 bits (76), Expect = 0.12
Identities = 27/74 (36%), Positives = 34/74 (45%), Gaps = 15/74 (20%)
Query: 27 AAEEPGGRAVFRAFRRANAR-------CFWNARLARAASRLAFQGWLRRGVLLVRAPPAC 79
A+ GGR V +A + AR FW AR ASR W RR +L +R+P A
Sbjct: 196 ASGTTGGRRVSKALMASMARRASRGPIAFW----ARRASRTRLAAWARRALLSLRSPKA- 250
Query: 80 LQVLRDAWRRRALR 93
R RRRA +
Sbjct: 251 ---RRGKARRRAAK 261
>gi|41350320 melanoma antigen family D, 2 [Homo sapiens]
Length = 606
Score = 33.9 bits (76), Expect = 0.12
Identities = 27/74 (36%), Positives = 34/74 (45%), Gaps = 15/74 (20%)
Query: 27 AAEEPGGRAVFRAFRRANAR-------CFWNARLARAASRLAFQGWLRRGVLLVRAPPAC 79
A+ GGR V +A + AR FW AR ASR W RR +L +R+P A
Sbjct: 196 ASGTTGGRRVSKALMASMARRASRGPIAFW----ARRASRTRLAAWARRALLSLRSPKA- 250
Query: 80 LQVLRDAWRRRALR 93
R RRRA +
Sbjct: 251 ---RRGKARRRAAK 261
>gi|110611172 amnionless protein precursor [Homo sapiens]
Length = 453
Score = 32.0 bits (71), Expect = 0.45
Identities = 33/105 (31%), Positives = 40/105 (38%), Gaps = 6/105 (5%)
Query: 18 RLEAQKAAGAAEEPGGRAVFRAFRRANARCFWNARLARAASRLAFQGWLRRGVLLVRAPP 77
RL A AE V A R + W + A A +A L VLLV APP
Sbjct: 323 RLARALLADVAENGEALGVLEATMRESGAHVWGSSAAGLAGGVAAAVLLALLVLLV-APP 381
Query: 78 ACLQVLRDAWRRRALRPPRGFRIRAVGDVFPVQMNPITQSQFVPL 122
+ R WRR P G + VF V T S+ +PL
Sbjct: 382 LLRRAGRLRWRRHEAAAPAGAPLGFRNPVFDV-----TASEELPL 421
>gi|239755540 PREDICTED: hypothetical protein [Homo sapiens]
Length = 170
Score = 28.5 bits (62), Expect = 4.9
Identities = 23/79 (29%), Positives = 33/79 (41%), Gaps = 5/79 (6%)
Query: 78 ACLQVLRDAWRRRALRPPR-----GFRIRAVGDVFPVQMNPITQSQFVPLGEVLCCAISD 132
ACL + +AW R R P+ G RAV P+ M P Q P G A +
Sbjct: 76 ACLSIPPEAWSSRRSRHPQAVTAAGEATRAVVGWLPMDMCPSPTWQDPPRGRAFHRAENR 135
Query: 133 MNTAQIVVTQESLLERLMK 151
+ V Q SL+ +++
Sbjct: 136 QRRNSLEVAQISLVAPVLQ 154
>gi|239755457 PREDICTED: similar to patatin-like phospholipase
domain containing 2 [Homo sapiens]
Length = 226
Score = 28.5 bits (62), Expect = 4.9
Identities = 23/75 (30%), Positives = 30/75 (40%), Gaps = 5/75 (6%)
Query: 53 LARAASRLAFQGWLRR----GVLLVRAPPACLQVLRDAWRRRALRPPRGFRIRAVGDVFP 108
L+ AA R A GW+R G L + Q+L + PP R+RA D P
Sbjct: 132 LSCAAYREALPGWMRNNLSLGDALAKWEECQRQLLLGLFCTNVAFPPEALRMRAPADPAP 191
Query: 109 VQMNPITQSQFVPLG 123
+P Q P G
Sbjct: 192 APADP-ASPQHQPAG 205
>gi|239749992 PREDICTED: hypothetical protein [Homo sapiens]
Length = 170
Score = 28.5 bits (62), Expect = 4.9
Identities = 23/79 (29%), Positives = 33/79 (41%), Gaps = 5/79 (6%)
Query: 78 ACLQVLRDAWRRRALRPPR-----GFRIRAVGDVFPVQMNPITQSQFVPLGEVLCCAISD 132
ACL + +AW R R P+ G RAV P+ M P Q P G A +
Sbjct: 76 ACLSIPPEAWSSRRSRHPQAVTAAGEATRAVVGWLPMDMCPSPTWQDPPRGRAFHRAENR 135
Query: 133 MNTAQIVVTQESLLERLMK 151
+ V Q SL+ +++
Sbjct: 136 QRRNSLEVAQISLVAPVLQ 154
>gi|239744303 PREDICTED: hypothetical protein XP_002343093 [Homo
sapiens]
Length = 170
Score = 28.5 bits (62), Expect = 4.9
Identities = 23/79 (29%), Positives = 33/79 (41%), Gaps = 5/79 (6%)
Query: 78 ACLQVLRDAWRRRALRPPR-----GFRIRAVGDVFPVQMNPITQSQFVPLGEVLCCAISD 132
ACL + +AW R R P+ G RAV P+ M P Q P G A +
Sbjct: 76 ACLSIPPEAWSSRRSRHPQAVTAAGEATRAVVGWLPMDMCPSPTWQDPPRGRAFHRAENR 135
Query: 133 MNTAQIVVTQESLLERLMK 151
+ V Q SL+ +++
Sbjct: 136 QRRNSLEVAQISLVAPVLQ 154
>gi|169169855 PREDICTED: similar to hCG34808 [Homo sapiens]
Length = 483
Score = 28.1 bits (61), Expect = 6.4
Identities = 17/52 (32%), Positives = 27/52 (51%), Gaps = 1/52 (1%)
Query: 130 ISDMNTAQIVVTQESLLERLMKHYPGIAIPSEDILYTTLGTLIKERKIYHTG 181
++++NT Q T +SL + K++ IP +G L K+R IYH G
Sbjct: 88 LAELNTCQRQETADSLDNKKKKYFSLEEIPKSSKTVFIIGNLQKKR-IYHLG 138
>gi|239752201 PREDICTED: hypothetical protein XP_002348147 [Homo
sapiens]
Length = 935
Score = 28.1 bits (61), Expect = 6.4
Identities = 28/85 (32%), Positives = 34/85 (40%), Gaps = 7/85 (8%)
Query: 18 RLEAQKAAGAAEEPGGRAVFRAFRRANARCFWNARLARAASRLAFQGWLRRGVLLVRA-- 75
+L G A GR V R R A + RAASR A +G RR + A
Sbjct: 796 KLTDSARGGRARLTAGRRVRRESGRRRAGATSQGAIRRAASR-ARKGESRRPLPANSAGR 854
Query: 76 ----PPACLQVLRDAWRRRALRPPR 96
PA + R +W RA RP R
Sbjct: 855 PALPSPAAARSARGSWGLRASRPSR 879
>gi|169168879 PREDICTED: similar to hCG34808 [Homo sapiens]
Length = 483
Score = 28.1 bits (61), Expect = 6.4
Identities = 17/52 (32%), Positives = 27/52 (51%), Gaps = 1/52 (1%)
Query: 130 ISDMNTAQIVVTQESLLERLMKHYPGIAIPSEDILYTTLGTLIKERKIYHTG 181
++++NT Q T +SL + K++ IP +G L K+R IYH G
Sbjct: 88 LAELNTCQRQETADSLDNKKKKYFSLEEIPKSSKTVFIIGNLQKKR-IYHLG 138
>gi|169169326 PREDICTED: similar to hCG34808 [Homo sapiens]
Length = 483
Score = 28.1 bits (61), Expect = 6.4
Identities = 17/52 (32%), Positives = 27/52 (51%), Gaps = 1/52 (1%)
Query: 130 ISDMNTAQIVVTQESLLERLMKHYPGIAIPSEDILYTTLGTLIKERKIYHTG 181
++++NT Q T +SL + K++ IP +G L K+R IYH G
Sbjct: 88 LAELNTCQRQETADSLDNKKKKYFSLEEIPKSSKTVFIIGNLQKKR-IYHLG 138
>gi|32698724 patatin-like phospholipase domain containing 2 [Homo
sapiens]
Length = 504
Score = 28.1 bits (61), Expect = 6.4
Identities = 20/65 (30%), Positives = 27/65 (41%), Gaps = 4/65 (6%)
Query: 53 LARAASRLAFQGWLRR----GVLLVRAPPACLQVLRDAWRRRALRPPRGFRIRAVGDVFP 108
L+ AA R A GW+R G L + Q+L + PP R+RA D P
Sbjct: 410 LSCAAYREALPGWMRNNLSLGDALAKWEECQRQLLLGLFCTNVAFPPEALRMRAPADPAP 469
Query: 109 VQMNP 113
+P
Sbjct: 470 APADP 474
>gi|215422401 hypothetical protein LOC9811 isoform 2 [Homo sapiens]
Length = 600
Score = 28.1 bits (61), Expect = 6.4
Identities = 23/76 (30%), Positives = 36/76 (47%), Gaps = 9/76 (11%)
Query: 102 AVGDVFPVQMNPI-------TQSQFVPLGEVLCCAISDMNTAQIVVTQ--ESLLERLMKH 152
+ G+ F V + PI QSQ V VLCC++ +T +++ Q E + E L
Sbjct: 493 STGEPFRVLVCPIYTCLRELLQSQDVKEDAVLCCSMELQSTGRLLEEQLPEMMTELLASA 552
Query: 153 YPGIAIPSEDILYTTL 168
+ PSE +L +L
Sbjct: 553 RDKMLCPSESMLTRSL 568
>gi|7662112 hypothetical protein LOC9811 isoform 1 [Homo sapiens]
Length = 598
Score = 28.1 bits (61), Expect = 6.4
Identities = 23/76 (30%), Positives = 36/76 (47%), Gaps = 9/76 (11%)
Query: 102 AVGDVFPVQMNPI-------TQSQFVPLGEVLCCAISDMNTAQIVVTQ--ESLLERLMKH 152
+ G+ F V + PI QSQ V VLCC++ +T +++ Q E + E L
Sbjct: 491 STGEPFRVLVCPIYTCLRELLQSQDVKEDAVLCCSMELQSTGRLLEEQLPEMMTELLASA 550
Query: 153 YPGIAIPSEDILYTTL 168
+ PSE +L +L
Sbjct: 551 RDKMLCPSESMLTRSL 566
>gi|31542713 MOCO sulphurase C-terminal domain containing 2 [Homo
sapiens]
Length = 335
Score = 28.1 bits (61), Expect = 6.4
Identities = 16/45 (35%), Positives = 24/45 (53%), Gaps = 2/45 (4%)
Query: 86 AWRRRALRPPRGFRIRAVGDVFPVQMNPITQSQFVPLGEVLCCAI 130
AWRR P R R++ VG V + + P+ + VP+ E C A+
Sbjct: 39 AWRRAW--PRRRRRLQQVGTVAKLWIYPVKSCKGVPVSEAECTAM 81
Database: hs.faa
Posted date: Aug 4, 2009 4:42 PM
Number of letters in database: 18,247,518
Number of sequences in database: 37,866
Lambda K H
0.325 0.137 0.415
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,544,054
Number of Sequences: 37866
Number of extensions: 346311
Number of successful extensions: 1003
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 16
Number of HSP's that attempted gapping in prelim test: 993
Number of HSP's gapped (non-prelim): 24
length of query: 227
length of database: 18,247,518
effective HSP length: 99
effective length of query: 128
effective length of database: 14,498,784
effective search space: 1855844352
effective search space used: 1855844352
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 60 (27.7 bits)
Search results were obtained with NCBI BLAST and RefSeq entries.
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Guide to the Human Genome
Copyright © 2010 by Stewart Scherer. All rights reserved.