Guide to the Human Genome
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Search of human proteins with 62865628

BLASTP 2.2.11 [Jun-05-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|62865628 sterol-C4-methyl oxidase-like isoform 2 [Homo
sapiens]
         (162 letters)

Database: hs.faa 
           37,866 sequences; 18,247,518 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|62865628 sterol-C4-methyl oxidase-like isoform 2 [Homo sapiens]    358   1e-99
gi|5803157 sterol-C4-methyl oxidase-like isoform 1 [Homo sapiens]     358   1e-99
gi|4502499 cholesterol 25-hydroxylase [Homo sapiens]                   92   1e-19
gi|146198575 hypothetical protein LOC10826 isoform 2 [Homo sapiens]    88   3e-18
gi|68160945 sterol-C5-desaturase [Homo sapiens]                        81   3e-16
gi|68160941 sterol-C5-desaturase [Homo sapiens]                        81   3e-16
gi|51972212 transmembrane protein 195 [Homo sapiens]                   40   7e-04
gi|205360949 fatty acid 2-hydroxylase [Homo sapiens]                   29   2.0  
gi|209976994 adenylate cyclase 10 [Homo sapiens]                       28   4.5  
gi|119220564 one cut domain, family member 2 [Homo sapiens]            28   4.5  
gi|24307887 one cut homeobox 1 [Homo sapiens]                          27   5.9  
gi|4502141 baculoviral IAP repeat-containing protein 2 [Homo sap...    27   7.7  

>gi|62865628 sterol-C4-methyl oxidase-like isoform 2 [Homo sapiens]
          Length = 162

 Score =  358 bits (918), Expect = 1e-99
 Identities = 162/162 (100%), Positives = 162/162 (100%)

Query: 1   MPRWYFLLARCFGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLE 60
           MPRWYFLLARCFGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLE
Sbjct: 1   MPRWYFLLARCFGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLE 60

Query: 61  TLILGTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHD 120
           TLILGTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHD
Sbjct: 61  TLILGTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHD 120

Query: 121 FHHMNFIGNYASTFTWWDRIFGTDSQYNAYNEKRKKFEKKTE 162
           FHHMNFIGNYASTFTWWDRIFGTDSQYNAYNEKRKKFEKKTE
Sbjct: 121 FHHMNFIGNYASTFTWWDRIFGTDSQYNAYNEKRKKFEKKTE 162


>gi|5803157 sterol-C4-methyl oxidase-like isoform 1 [Homo sapiens]
          Length = 293

 Score =  358 bits (918), Expect = 1e-99
 Identities = 162/162 (100%), Positives = 162/162 (100%)

Query: 1   MPRWYFLLARCFGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLE 60
           MPRWYFLLARCFGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLE
Sbjct: 132 MPRWYFLLARCFGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLE 191

Query: 61  TLILGTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHD 120
           TLILGTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHD
Sbjct: 192 TLILGTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHD 251

Query: 121 FHHMNFIGNYASTFTWWDRIFGTDSQYNAYNEKRKKFEKKTE 162
           FHHMNFIGNYASTFTWWDRIFGTDSQYNAYNEKRKKFEKKTE
Sbjct: 252 FHHMNFIGNYASTFTWWDRIFGTDSQYNAYNEKRKKFEKKTE 293


>gi|4502499 cholesterol 25-hydroxylase [Homo sapiens]
          Length = 272

 Score = 92.4 bits (228), Expect = 1e-19
 Identities = 50/146 (34%), Positives = 75/146 (51%), Gaps = 5/146 (3%)

Query: 2   PRWYFLLARCFGCAVIEDTWHYFLHRLLHHKR--IYKYIHKVHHEFQAPFGMEAEYAHPL 59
           P    LL     C ++ D   +F+  LLHHK   +Y+  HKVHH+  + F +  +Y    
Sbjct: 119 PELLLLLHHILFCLLLFDM-EFFVWHLLHHKVPWLYRTFHKVHHQNSSSFALATQYMSVW 177

Query: 60  ETLILGTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNLIPF--YAGSR 117
           E   LG    + + LL  H +    +  + +  +++ HSGY+ P +   L+PF  Y G  
Sbjct: 178 ELFSLGFFDMMNVTLLGCHPLTTLTFHVVNIWLSVEDHSGYNFPWSTHRLVPFGWYGGVV 237

Query: 118 HHDFHHMNFIGNYASTFTWWDRIFGT 143
           HHD HH +F  N+A  FT WD+I GT
Sbjct: 238 HHDLHHSHFNCNFAPYFTHWDKILGT 263


>gi|146198575 hypothetical protein LOC10826 isoform 2 [Homo sapiens]
          Length = 333

 Score = 88.2 bits (217), Expect = 3e-18
 Identities = 47/148 (31%), Positives = 74/148 (50%), Gaps = 10/148 (6%)

Query: 1   MPRWYFLLARCFGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLE 60
           +P +++ L       +IE+   Y+ HRLLHH   YK IHK HHE+ AP G+ + YAHP+E
Sbjct: 165 LPTFHWFLLELAIFTLIEEVLFYYSHRLLHHPTFYKKIHKKHHEWTAPIGVISLYAHPIE 224

Query: 61  TLILG-TGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHH 119
             +       +G +++  H+  +  W ++ L+ T   H GY         +PF      H
Sbjct: 225 HAVSNMLPVIVGPLVMGSHLSSITMWFSLALIITTISHCGYH--------LPFLPSPEFH 276

Query: 120 DFHHMNFIGNYASTFTWWDRIFGTDSQY 147
           D+HH+ F   Y       D + GTD+ +
Sbjct: 277 DYHHLKFNQCY-GVLGVLDHLHGTDTMF 303


>gi|68160945 sterol-C5-desaturase [Homo sapiens]
          Length = 299

 Score = 81.3 bits (199), Expect = 3e-16
 Identities = 45/135 (33%), Positives = 70/135 (51%), Gaps = 2/135 (1%)

Query: 19  DTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLILGTGFFIGIVLLCDH 78
           D + Y++HR LHH+ +YK +HK HH ++ P    +   HP++  +    + I   +   H
Sbjct: 131 DMFIYWIHRGLHHRLVYKRLHKPHHIWKIPTPFASHAFHPIDGFLQSLPYHIYPFIFPLH 190

Query: 79  VILLWAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHDFHHMNFIGNYASTFTWWD 138
            ++  +   +  + TI +H G D  + P  L PF  GS HH  HHM F  NY   FT WD
Sbjct: 191 KVVYLSLYILVNIWTISIHDG-DFRV-PQILQPFINGSAHHTDHHMFFDYNYGQYFTLWD 248

Query: 139 RIFGTDSQYNAYNEK 153
           RI G+    +++  K
Sbjct: 249 RIGGSFKNPSSFEGK 263


>gi|68160941 sterol-C5-desaturase [Homo sapiens]
          Length = 299

 Score = 81.3 bits (199), Expect = 3e-16
 Identities = 45/135 (33%), Positives = 70/135 (51%), Gaps = 2/135 (1%)

Query: 19  DTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLILGTGFFIGIVLLCDH 78
           D + Y++HR LHH+ +YK +HK HH ++ P    +   HP++  +    + I   +   H
Sbjct: 131 DMFIYWIHRGLHHRLVYKRLHKPHHIWKIPTPFASHAFHPIDGFLQSLPYHIYPFIFPLH 190

Query: 79  VILLWAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHDFHHMNFIGNYASTFTWWD 138
            ++  +   +  + TI +H G D  + P  L PF  GS HH  HHM F  NY   FT WD
Sbjct: 191 KVVYLSLYILVNIWTISIHDG-DFRV-PQILQPFINGSAHHTDHHMFFDYNYGQYFTLWD 248

Query: 139 RIFGTDSQYNAYNEK 153
           RI G+    +++  K
Sbjct: 249 RIGGSFKNPSSFEGK 263


>gi|51972212 transmembrane protein 195 [Homo sapiens]
          Length = 445

 Score = 40.4 bits (93), Expect = 7e-04
 Identities = 36/139 (25%), Positives = 53/139 (38%), Gaps = 11/139 (7%)

Query: 19  DTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLILGTGFFIGIVLLCDH 78
           D  +Y+ HR+ H   I    H+ HH  +  + +       +  +     F+  + L    
Sbjct: 125 DFGYYWFHRMAHEVNIMWAGHQTHHSSE-DYNLSTALRQSVLQIYTSWIFYSPLALFIPP 183

Query: 79  VILLWAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHDFHHMN----FIGNYASTF 134
            +     +   LL    +H+     L PL LI       HH  HH         NYA   
Sbjct: 184 SVYA-VHLQFNLLYQFWIHTEVINNLGPLELI--LNTPSHHRVHHGRNRYCIDKNYAGVL 240

Query: 135 TWWDRIFGTDSQYNAYNEK 153
             WD+IFGT   + A NEK
Sbjct: 241 IIWDKIFGT---FEAENEK 256


>gi|205360949 fatty acid 2-hydroxylase [Homo sapiens]
          Length = 372

 Score = 28.9 bits (63), Expect = 2.0
 Identities = 13/28 (46%), Positives = 15/28 (53%)

Query: 16  VIEDTWHYFLHRLLHHKRIYKYIHKVHH 43
           V+ D  HY+LH    HK  Y Y  K HH
Sbjct: 309 VLYDMTHYYLHFGSPHKGSYLYSLKAHH 336


>gi|209976994 adenylate cyclase 10 [Homo sapiens]
          Length = 1610

 Score = 27.7 bits (60), Expect = 4.5
 Identities = 15/55 (27%), Positives = 24/55 (43%)

Query: 107  LNLIPFYAGSRHHDFHHMNFIGNYASTFTWWDRIFGTDSQYNAYNEKRKKFEKKT 161
            L  I  Y  +R   FH    +G Y+S   W+ R+   D+ Y   N  +    ++T
Sbjct: 1400 LEFIHQYENNRILKFHSGLLLGLYSSVAIWYARLQEWDNFYKFSNRAKNLLPRRT 1454


>gi|119220564 one cut domain, family member 2 [Homo sapiens]
          Length = 504

 Score = 27.7 bits (60), Expect = 4.5
 Identities = 15/57 (26%), Positives = 23/57 (40%), Gaps = 8/57 (14%)

Query: 103 PLNPLNLI--------PFYAGSRHHDFHHMNFIGNYASTFTWWDRIFGTDSQYNAYN 151
           PL P++ +        P +    HH  HH    GN + +FT      G  +  N Y+
Sbjct: 156 PLPPISTVSDKFHHPHPHHHPHHHHHHHHQRLSGNVSGSFTLMRDERGLPAMNNLYS 212


>gi|24307887 one cut homeobox 1 [Homo sapiens]
          Length = 465

 Score = 27.3 bits (59), Expect = 5.9
 Identities = 13/38 (34%), Positives = 15/38 (39%)

Query: 118 HHDFHHMNFIGNYASTFTWWDRIFGTDSQYNAYNEKRK 155
           HH  HH    GN + +FT      G  S  N Y    K
Sbjct: 133 HHPHHHQRLAGNVSGSFTLMRDERGLASMNNLYTPYHK 170


>gi|4502141 baculoviral IAP repeat-containing protein 2 [Homo
           sapiens]
          Length = 618

 Score = 26.9 bits (58), Expect = 7.7
 Identities = 16/70 (22%), Positives = 27/70 (38%), Gaps = 8/70 (11%)

Query: 10  RCFGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFG--------MEAEYAHPLET 61
           +CF C ++ D W      +  HK++Y     + +   A  G        M   +AH L  
Sbjct: 82  KCFCCGLMLDNWKLGDSPIQKHKQLYPSCSFIQNLVSASLGSTSKNTSPMRNSFAHSLSP 141

Query: 62  LILGTGFFIG 71
            +  +  F G
Sbjct: 142 TLEHSSLFSG 151


  Database: hs.faa
    Posted date:  Aug 4, 2009  4:42 PM
  Number of letters in database: 18,247,518
  Number of sequences in database:  37,866
  
Lambda     K      H
   0.330    0.145    0.506 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,598,827
Number of Sequences: 37866
Number of extensions: 286021
Number of successful extensions: 611
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 594
Number of HSP's gapped (non-prelim): 13
length of query: 162
length of database: 18,247,518
effective HSP length: 94
effective length of query: 68
effective length of database: 14,688,114
effective search space: 998791752
effective search space used: 998791752
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 58 (26.9 bits)

Search results were obtained with NCBI BLAST and RefSeq entries.


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Guide to the Human Genome
Copyright © 2010 by Stewart Scherer. All rights reserved.

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