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Search of human proteins with 23503259
BLASTP 2.2.11 [Jun-05-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= gi|23503259 pseudouridylate synthase-like 1 [Homo sapiens]
(303 letters)
Database: hs.faa
37,866 sequences; 18,247,518 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|23503259 pseudouridylate synthase-like 1 [Homo sapiens] 606 e-174
gi|199559785 pseudouridylate synthase 3 [Homo sapiens] 64 2e-10
gi|70166634 pseudouridine synthase 1 isoform 2 [Homo sapiens] 59 5e-09
gi|70166599 pseudouridine synthase 1 isoform 2 [Homo sapiens] 59 5e-09
gi|70166645 pseudouridine synthase 1 isoform 1 [Homo sapiens] 59 5e-09
gi|23943912 phosphoinositide-3-kinase, regulatory subunit 4 [Hom... 31 1.2
gi|19923973 potassium channel tetramerisation domain containing ... 30 3.4
gi|4505731 peroxisomal biogenesis factor 7 [Homo sapiens] 29 5.9
gi|239757099 PREDICTED: hypothetical protein [Homo sapiens] 28 7.7
gi|239751605 PREDICTED: hypothetical protein XP_002347934 [Homo ... 28 7.7
gi|239746122 PREDICTED: hypothetical protein XP_002344202 [Homo ... 28 7.7
>gi|23503259 pseudouridylate synthase-like 1 [Homo sapiens]
Length = 303
Score = 606 bits (1562), Expect = e-174
Identities = 303/303 (100%), Positives = 303/303 (100%)
Query: 1 MSSAPASGSVRARYLVYFQYVGTDFNGVAAVRGTQRAVGVQNYLEEAAERLNSVEPVRFT 60
MSSAPASGSVRARYLVYFQYVGTDFNGVAAVRGTQRAVGVQNYLEEAAERLNSVEPVRFT
Sbjct: 1 MSSAPASGSVRARYLVYFQYVGTDFNGVAAVRGTQRAVGVQNYLEEAAERLNSVEPVRFT 60
Query: 61 ISSRTDAGVHALSNAAHLDVQRRSGRPPFPPEVLAEALNTHLRHPAIRVLRAFRVPSDFH 120
ISSRTDAGVHALSNAAHLDVQRRSGRPPFPPEVLAEALNTHLRHPAIRVLRAFRVPSDFH
Sbjct: 61 ISSRTDAGVHALSNAAHLDVQRRSGRPPFPPEVLAEALNTHLRHPAIRVLRAFRVPSDFH 120
Query: 121 ARHAATSRTYLYRLATGCHRRDELPVFERNLCWTLPADCLDMVAMQEAAQHLLGTHDFSA 180
ARHAATSRTYLYRLATGCHRRDELPVFERNLCWTLPADCLDMVAMQEAAQHLLGTHDFSA
Sbjct: 121 ARHAATSRTYLYRLATGCHRRDELPVFERNLCWTLPADCLDMVAMQEAAQHLLGTHDFSA 180
Query: 181 FQSAGSPVPSPVRTLRRVSVSPGQASPLVTPEESRKLRFWNLEFESQSFLYRQVRRMTAV 240
FQSAGSPVPSPVRTLRRVSVSPGQASPLVTPEESRKLRFWNLEFESQSFLYRQVRRMTAV
Sbjct: 181 FQSAGSPVPSPVRTLRRVSVSPGQASPLVTPEESRKLRFWNLEFESQSFLYRQVRRMTAV 240
Query: 241 LVAVGLGALAPAQVKTILESQDPLGKHQTRVAPAHGLFLKSVLYGNLGAASCTLQGPQFG 300
LVAVGLGALAPAQVKTILESQDPLGKHQTRVAPAHGLFLKSVLYGNLGAASCTLQGPQFG
Sbjct: 241 LVAVGLGALAPAQVKTILESQDPLGKHQTRVAPAHGLFLKSVLYGNLGAASCTLQGPQFG 300
Query: 301 SHG 303
SHG
Sbjct: 301 SHG 303
>gi|199559785 pseudouridylate synthase 3 [Homo sapiens]
Length = 481
Score = 63.9 bits (154), Expect = 2e-10
Identities = 76/287 (26%), Positives = 108/287 (37%), Gaps = 44/287 (15%)
Query: 20 YVGTDFNGVAAVRGTQRAVGVQNYLEEAAERLNSVEPVR---FTISSRTDAGVHALSNAA 76
Y+G + G A+ T + + L EA + VE + + RTD GV A
Sbjct: 71 YMGWGYQGFASQENTNNTI--EEKLFEALTKTRLVESRQTSNYHRCGRTDKGVSAFGQVI 128
Query: 77 HLDVQRRSGR----PPFPPEVLAEALNTHLRH---------PAIRVLRAFRVPSDFHARH 123
LD++ + R F + A A +R+ P IR+L V F AR
Sbjct: 129 SLDLRSQFPRGRDSEDFNVKEEANAAAEEIRYTHILNRVLPPDIRILAWAPVEPSFSARF 188
Query: 124 AATSRTYLYRLATGCHRRDELPVFERNLCWTLPADCLDMVAMQEAAQHLLGTHDFSAFQS 183
+ RTY Y P LD+V M AAQ +GTHDF
Sbjct: 189 SCLERTYRY---------------------FFPRADLDIVTMDYAAQKYVGTHDFRNL-- 225
Query: 184 AGSPVPSPVRTLRRVSVSPGQASPLVTPEESR---KLRFWNLEFESQSFLYRQVRRMTAV 240
V + V +R +S +P E R + E Q+FLY QVR M A+
Sbjct: 226 CKMDVANGVINFQRTILSAQVQLVGQSPGEGRWQEPFQLCQFEVTGQAFLYHQVRCMMAI 285
Query: 241 LVAVGLGALAPAQVKTILESQDPLGKHQTRVAPAHGLFLKSVLYGNL 287
L +G G P + +L + K Q +A L L + N+
Sbjct: 286 LFLIGQGMEKPEIIDELLNIEKNPQKPQYSMAVEFPLVLYDCKFENV 332
>gi|70166634 pseudouridine synthase 1 isoform 2 [Homo sapiens]
Length = 399
Score = 58.9 bits (141), Expect = 5e-09
Identities = 71/281 (25%), Positives = 116/281 (41%), Gaps = 34/281 (12%)
Query: 11 RARYLVYFQYVGTDFNGVAAVRGTQRAVGVQNYLEEAAERLNSV--------EPVRFTIS 62
+ + ++ Y G ++G+ G+ + +++ L A R + + F
Sbjct: 55 KRKIVLLMAYSGKGYHGMQRNVGSSQFKTIEDDLVSALVRSGCIPENHGEDMRKMSFQRC 114
Query: 63 SRTDAGVHALSNAAHLDVQRRSGRPPFPPEVLAEALNTHLRHPAIRVLRAFRVPSDFHAR 122
+RTD GV A L V + + + E +N+HL IR+L RV F+++
Sbjct: 115 ARTDKGVSAAGQVVSLKV--------WLIDDILEKINSHLPSH-IRILGLKRVTGGFNSK 165
Query: 123 HAATSRTYLYRLATGCHRRDELPVFERNLCWTLPADCLDMVAMQEAAQHLLGTHDFSAFQ 182
+ +RTY Y L T + V + + L A+ L V A GTH+F F
Sbjct: 166 NRCDARTYCYLLPTFAFAHKDRDVQDET--YRLSAETLQQVNRLLACYK--GTHNFHNFT 221
Query: 183 SAGSPV-PSPVRTLRRVSVSPGQASPLVTPEESRKLRFWNLEFESQSFLYRQVRRMTAVL 241
S P PS R + + P L F + + QSF+ Q+R+M ++
Sbjct: 222 SQKGPQDPSACRYILEMYCE--------EPFVREGLEFAVIRVKGQSFMMHQIRKMVGLV 273
Query: 242 VAVGLGALAPAQVKTILESQDPLGKHQTRVAPAHGLFLKSV 282
VA+ G AP +++LE K AP GL L+ V
Sbjct: 274 VAIVKG-YAP---ESVLERSWGTEKVDVPKAPGLGLVLERV 310
>gi|70166599 pseudouridine synthase 1 isoform 2 [Homo sapiens]
Length = 399
Score = 58.9 bits (141), Expect = 5e-09
Identities = 71/281 (25%), Positives = 116/281 (41%), Gaps = 34/281 (12%)
Query: 11 RARYLVYFQYVGTDFNGVAAVRGTQRAVGVQNYLEEAAERLNSV--------EPVRFTIS 62
+ + ++ Y G ++G+ G+ + +++ L A R + + F
Sbjct: 55 KRKIVLLMAYSGKGYHGMQRNVGSSQFKTIEDDLVSALVRSGCIPENHGEDMRKMSFQRC 114
Query: 63 SRTDAGVHALSNAAHLDVQRRSGRPPFPPEVLAEALNTHLRHPAIRVLRAFRVPSDFHAR 122
+RTD GV A L V + + + E +N+HL IR+L RV F+++
Sbjct: 115 ARTDKGVSAAGQVVSLKV--------WLIDDILEKINSHLPSH-IRILGLKRVTGGFNSK 165
Query: 123 HAATSRTYLYRLATGCHRRDELPVFERNLCWTLPADCLDMVAMQEAAQHLLGTHDFSAFQ 182
+ +RTY Y L T + V + + L A+ L V A GTH+F F
Sbjct: 166 NRCDARTYCYLLPTFAFAHKDRDVQDET--YRLSAETLQQVNRLLACYK--GTHNFHNFT 221
Query: 183 SAGSPV-PSPVRTLRRVSVSPGQASPLVTPEESRKLRFWNLEFESQSFLYRQVRRMTAVL 241
S P PS R + + P L F + + QSF+ Q+R+M ++
Sbjct: 222 SQKGPQDPSACRYILEMYCE--------EPFVREGLEFAVIRVKGQSFMMHQIRKMVGLV 273
Query: 242 VAVGLGALAPAQVKTILESQDPLGKHQTRVAPAHGLFLKSV 282
VA+ G AP +++LE K AP GL L+ V
Sbjct: 274 VAIVKG-YAP---ESVLERSWGTEKVDVPKAPGLGLVLERV 310
>gi|70166645 pseudouridine synthase 1 isoform 1 [Homo sapiens]
Length = 427
Score = 58.9 bits (141), Expect = 5e-09
Identities = 71/281 (25%), Positives = 116/281 (41%), Gaps = 34/281 (12%)
Query: 11 RARYLVYFQYVGTDFNGVAAVRGTQRAVGVQNYLEEAAERLNSV--------EPVRFTIS 62
+ + ++ Y G ++G+ G+ + +++ L A R + + F
Sbjct: 83 KRKIVLLMAYSGKGYHGMQRNVGSSQFKTIEDDLVSALVRSGCIPENHGEDMRKMSFQRC 142
Query: 63 SRTDAGVHALSNAAHLDVQRRSGRPPFPPEVLAEALNTHLRHPAIRVLRAFRVPSDFHAR 122
+RTD GV A L V + + + E +N+HL IR+L RV F+++
Sbjct: 143 ARTDKGVSAAGQVVSLKV--------WLIDDILEKINSHLPSH-IRILGLKRVTGGFNSK 193
Query: 123 HAATSRTYLYRLATGCHRRDELPVFERNLCWTLPADCLDMVAMQEAAQHLLGTHDFSAFQ 182
+ +RTY Y L T + V + + L A+ L V A GTH+F F
Sbjct: 194 NRCDARTYCYLLPTFAFAHKDRDVQDET--YRLSAETLQQVNRLLACYK--GTHNFHNFT 249
Query: 183 SAGSPV-PSPVRTLRRVSVSPGQASPLVTPEESRKLRFWNLEFESQSFLYRQVRRMTAVL 241
S P PS R + + P L F + + QSF+ Q+R+M ++
Sbjct: 250 SQKGPQDPSACRYILEMYCE--------EPFVREGLEFAVIRVKGQSFMMHQIRKMVGLV 301
Query: 242 VAVGLGALAPAQVKTILESQDPLGKHQTRVAPAHGLFLKSV 282
VA+ G AP +++LE K AP GL L+ V
Sbjct: 302 VAIVKG-YAP---ESVLERSWGTEKVDVPKAPGLGLVLERV 338
>gi|23943912 phosphoinositide-3-kinase, regulatory subunit 4 [Homo
sapiens]
Length = 1358
Score = 31.2 bits (69), Expect = 1.2
Identities = 18/62 (29%), Positives = 33/62 (53%), Gaps = 6/62 (9%)
Query: 174 GTHDFSAFQSAGSPV----PSPVRTLRRVSVSPGQASP-LVTPEESRKLRFWNLEFESQS 228
G F+ + S+ P+ PSP ++ + SP +P L+T K+RFW+L + +S
Sbjct: 1218 GDRRFTLWASSAPPLSELQPSP-HSVHGIYCSPADGNPILLTAGSDMKIRFWDLAYPERS 1276
Query: 229 FL 230
++
Sbjct: 1277 YV 1278
>gi|19923973 potassium channel tetramerisation domain containing 12
[Homo sapiens]
Length = 325
Score = 29.6 bits (65), Expect = 3.4
Identities = 28/101 (27%), Positives = 43/101 (42%), Gaps = 10/101 (9%)
Query: 173 LGTHDFSAFQSAGSPVPSPVRTLRRVSVSPGQASPLVTPEE----SRKLRFWNLEFESQS 228
LG + + A + PSP L S S G A PL+TP + SR+ + + +
Sbjct: 159 LGYSEPEQQEGASAGAPSPTLELASRSPSGGAAGPLLTPSQSLDGSRRSGYITIGYRGSY 218
Query: 229 FLYRQVR-----RMTAVLVAVGLGALAPAQV-KTILESQDP 263
+ R + R A + G +LA T+ ES+DP
Sbjct: 219 TIGRDAQADAKFRRVARITVCGKTSLAKEVFGDTLNESRDP 259
>gi|4505731 peroxisomal biogenesis factor 7 [Homo sapiens]
Length = 323
Score = 28.9 bits (63), Expect = 5.9
Identities = 19/60 (31%), Positives = 24/60 (40%), Gaps = 2/60 (3%)
Query: 195 LRRVSVSPGQASPLVTPEESRKLRFWNLEFESQSFLYRQVRRMTAVLVAVGLGALAPAQV 254
+RRV SP AS L + +RFWN F L V T + +P QV
Sbjct: 245 IRRVKFSPFHASVLASCSYDFTVRFWN--FSKPDSLLETVEHHTEFTCGLDFSLQSPTQV 302
>gi|239757099 PREDICTED: hypothetical protein [Homo sapiens]
Length = 208
Score = 28.5 bits (62), Expect = 7.7
Identities = 16/56 (28%), Positives = 24/56 (42%)
Query: 163 VAMQEAAQHLLGTHDFSAFQSAGSPVPSPVRTLRRVSVSPGQASPLVTPEESRKLR 218
V Q A H L + GSP P+ RR ++ PG+ P +P + + R
Sbjct: 31 VQRQGTAGHDLRKGHRKQLEDTGSPAPTIRAHTRRKALGPGERGPEASPRPAPRAR 86
>gi|239751605 PREDICTED: hypothetical protein XP_002347934 [Homo
sapiens]
Length = 208
Score = 28.5 bits (62), Expect = 7.7
Identities = 16/56 (28%), Positives = 24/56 (42%)
Query: 163 VAMQEAAQHLLGTHDFSAFQSAGSPVPSPVRTLRRVSVSPGQASPLVTPEESRKLR 218
V Q A H L + GSP P+ RR ++ PG+ P +P + + R
Sbjct: 31 VQRQGTAGHDLRKGHRKQLEDTGSPAPTIRAHTRRKALGPGERGPEASPRPAPRAR 86
>gi|239746122 PREDICTED: hypothetical protein XP_002344202 [Homo
sapiens]
Length = 208
Score = 28.5 bits (62), Expect = 7.7
Identities = 16/56 (28%), Positives = 24/56 (42%)
Query: 163 VAMQEAAQHLLGTHDFSAFQSAGSPVPSPVRTLRRVSVSPGQASPLVTPEESRKLR 218
V Q A H L + GSP P+ RR ++ PG+ P +P + + R
Sbjct: 31 VQRQGTAGHDLRKGHRKQLEDTGSPAPTIRAHTRRKALGPGERGPEASPRPAPRAR 86
Database: hs.faa
Posted date: Aug 4, 2009 4:42 PM
Number of letters in database: 18,247,518
Number of sequences in database: 37,866
Lambda K H
0.321 0.133 0.398
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,567,502
Number of Sequences: 37866
Number of extensions: 517791
Number of successful extensions: 1405
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 1394
Number of HSP's gapped (non-prelim): 11
length of query: 303
length of database: 18,247,518
effective HSP length: 102
effective length of query: 201
effective length of database: 14,385,186
effective search space: 2891422386
effective search space used: 2891422386
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 62 (28.5 bits)
Search results were obtained with NCBI BLAST and RefSeq entries.
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Guide to the Human Genome
Copyright © 2010 by Stewart Scherer. All rights reserved.