Search of human proteins with 28178825

Search of human proteins with 28178825

BLASTP 2.2.11 [Jun-05-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|28178825 isocitrate dehydrogenase 1 (NADP+), soluble [Homo
sapiens]
         (414 letters)

Database: hs.faa 
           37,866 sequences; 18,247,518 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|28178825 isocitrate dehydrogenase 1 (NADP+), soluble [Homo sa...   842   0.0  
gi|28178832 isocitrate dehydrogenase 2 (NADP+), mitochondrial pr...   584   e-167
gi|90403579 beta-gamma crystallin domain containing 3 [Homo sapi...    33   0.62 
gi|114431246 keratin 25D [Homo sapiens]                                30   4.0  
gi|169790923 5-methyltetrahydrofolate-homocysteine methyltransfe...    30   5.2  
gi|238908505 DNA-directed RNA polymerase III B isoform 2 [Homo s...    29   6.9  
gi|238908503 DNA-directed RNA polymerase III B isoform 1 [Homo s...    29   6.9  
gi|21265040 mitochondrial ribosomal protein L22 isoform a [Homo ...    29   9.0  

>gi|28178825 isocitrate dehydrogenase 1 (NADP+), soluble [Homo
           sapiens]
          Length = 414

 Score =  842 bits (2174), Expect = 0.0
 Identities = 414/414 (100%), Positives = 414/414 (100%)

Query: 1   MSKKISGGSVVEMQGDEMTRIIWELIKEKLIFPYVELDLHSYDLGIENRDATNDQVTKDA 60
           MSKKISGGSVVEMQGDEMTRIIWELIKEKLIFPYVELDLHSYDLGIENRDATNDQVTKDA
Sbjct: 1   MSKKISGGSVVEMQGDEMTRIIWELIKEKLIFPYVELDLHSYDLGIENRDATNDQVTKDA 60

Query: 61  AEAIKKHNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRL 120
           AEAIKKHNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRL
Sbjct: 61  AEAIKKHNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRL 120

Query: 121 VSGWVKPIIIGRHAYGDQYRATDFVVPGPGKVEITYTPSDGTQKVTYLVHNFEEGGGVAM 180
           VSGWVKPIIIGRHAYGDQYRATDFVVPGPGKVEITYTPSDGTQKVTYLVHNFEEGGGVAM
Sbjct: 121 VSGWVKPIIIGRHAYGDQYRATDFVVPGPGKVEITYTPSDGTQKVTYLVHNFEEGGGVAM 180

Query: 181 GMYNQDKSIEDFAHSSFQMALSKGWPLYLSTKNTILKKYDGRFKDIFQEIYDKQYKSQFE 240
           GMYNQDKSIEDFAHSSFQMALSKGWPLYLSTKNTILKKYDGRFKDIFQEIYDKQYKSQFE
Sbjct: 181 GMYNQDKSIEDFAHSSFQMALSKGWPLYLSTKNTILKKYDGRFKDIFQEIYDKQYKSQFE 240

Query: 241 AQKIWYEHRLIDDMVAQAMKSEGGFIWACKNYDGDVQSDSVAQGYGSLGMMTSVLVCPDG 300
           AQKIWYEHRLIDDMVAQAMKSEGGFIWACKNYDGDVQSDSVAQGYGSLGMMTSVLVCPDG
Sbjct: 241 AQKIWYEHRLIDDMVAQAMKSEGGFIWACKNYDGDVQSDSVAQGYGSLGMMTSVLVCPDG 300

Query: 301 KTVEAEAAHGTVTRHYRMYQKGQETSTNPIASIFAWTRGLAHRAKLDNNKELAFFANALE 360
           KTVEAEAAHGTVTRHYRMYQKGQETSTNPIASIFAWTRGLAHRAKLDNNKELAFFANALE
Sbjct: 301 KTVEAEAAHGTVTRHYRMYQKGQETSTNPIASIFAWTRGLAHRAKLDNNKELAFFANALE 360

Query: 361 EVSIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDKLGENLKIKLAQAKL 414
           EVSIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDKLGENLKIKLAQAKL
Sbjct: 361 EVSIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDKLGENLKIKLAQAKL 414


>gi|28178832 isocitrate dehydrogenase 2 (NADP+), mitochondrial
           precursor [Homo sapiens]
          Length = 452

 Score =  584 bits (1505), Expect = e-167
 Identities = 281/397 (70%), Positives = 328/397 (82%), Gaps = 2/397 (0%)

Query: 10  VVEMQGDEMTRIIWELIKEKLIFPYVELDLHSYDLGIENRDATNDQVTKDAAEAIKKHNV 69
           VVEM GDEMTRIIW+ IKEKLI P+V++ L  +DLG+ NRD T+DQVT D+A A +K++V
Sbjct: 50  VVEMDGDEMTRIIWQFIKEKLILPHVDIQLKYFDLGLPNRDQTDDQVTIDSALATQKYSV 109

Query: 70  GVKCATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRLVSGWVKPII 129
            VKCATITPDE RVEEFKLK+MWKSPNGTIRNILGGTVFRE IICKNIPRLV GW KPI 
Sbjct: 110 AVKCATITPDEARVEEFKLKKMWKSPNGTIRNILGGTVFREPIICKNIPRLVPGWTKPIT 169

Query: 130 IGRHAYGDQYRATDFVVPGPGKVEITYTPSDGTQKVTYLVHNFEEGGGVAMGMYNQDKSI 189
           IGRHA+GDQY+ATDFV    G  ++ +TP DG+    + V+NF   GGV MGMYN D+SI
Sbjct: 170 IGRHAHGDQYKATDFVADRAGTFKMVFTPKDGSGVKEWEVYNFP-AGGVGMGMYNTDESI 228

Query: 190 EDFAHSSFQMALSKGWPLYLSTKNTILKKYDGRFKDIFQEIYDKQYKSQFEAQKIWYEHR 249
             FAHS FQ A+ K WPLY+STKNTILK YDGRFKDIFQEI+DK YK+ F+  KIWYEHR
Sbjct: 229 SGFAHSCFQYAIQKKWPLYMSTKNTILKAYDGRFKDIFQEIFDKHYKTDFDKNKIWYEHR 288

Query: 250 LIDDMVAQAMKSEGGFIWACKNYDGDVQSDSVAQGYGSLGMMTSVLVCPDGKTVEAEAAH 309
           LIDDMVAQ +KS GGF+WACKNYDGDVQSD +AQG+GSLG+MTSVLVCPDGKT+EAEAAH
Sbjct: 289 LIDDMVAQVLKSSGGFVWACKNYDGDVQSDILAQGFGSLGLMTSVLVCPDGKTIEAEAAH 348

Query: 310 GTVTRHYRMYQKGQETSTNPIASIFAWTRGLAHRAKLDNNKELAFFANALEEVSIETIEA 369
           GTVTRHYR +QKG+ TSTNPIASIFAWTRGL HR KLD N++L  FA  LE+V +ET+E+
Sbjct: 349 GTVTRHYREHQKGRPTSTNPIASIFAWTRGLEHRGKLDGNQDLIRFAQMLEKVCVETVES 408

Query: 370 GFMTKDLAACIKGLPNVQRSD-YLNTFEFMDKLGENL 405
           G MTKDLA CI GL NV+ ++ +LNT +F+D +  NL
Sbjct: 409 GAMTKDLAGCIHGLSNVKLNEHFLNTTDFLDTIKSNL 445


>gi|90403579 beta-gamma crystallin domain containing 3 [Homo
           sapiens]
          Length = 1022

 Score = 32.7 bits (73), Expect = 0.62
 Identities = 26/101 (25%), Positives = 43/101 (42%), Gaps = 9/101 (8%)

Query: 144 FVVPGPGKVEITYTPSD----GTQKVTYLVHNFEEGGGVAMGMYNQDKSI--EDFAHSSF 197
           F +P P    IT+ P D    G  K +Y++ N      + +G++ +  S   +    S  
Sbjct: 225 FQLPDPS---ITFYPDDQESVGISKNSYVMPNEPTTSNLQVGLWPEKTSFLQKSDLTSKL 281

Query: 198 QMALSKGWPLYLSTKNTILKKYDGRFKDIFQEIYDKQYKSQ 238
             +L   +  YL T  +   +   RF  IFQE   K ++ Q
Sbjct: 282 HSSLKSAYHQYLQTSQSHSSEKGARFGGIFQEPVSKYFRVQ 322


>gi|114431246 keratin 25D [Homo sapiens]
          Length = 464

 Score = 30.0 bits (66), Expect = 4.0
 Identities = 20/54 (37%), Positives = 30/54 (55%), Gaps = 5/54 (9%)

Query: 234 QYKSQFEAQKIWYEHRLIDDMVAQAMKSEGGFIWACKNYDGDVQSDSVAQGYGS 287
           Q +++ E QK+ YEH L+D  V    + E      C+  DGD  S S ++G+GS
Sbjct: 361 QVRTETEGQKLEYEH-LLDVKVHLEKEIET----YCRLIDGDGNSCSKSKGFGS 409


>gi|169790923 5-methyltetrahydrofolate-homocysteine
           methyltransferase [Homo sapiens]
          Length = 1265

 Score = 29.6 bits (65), Expect = 5.2
 Identities = 27/77 (35%), Positives = 35/77 (45%), Gaps = 4/77 (5%)

Query: 324 ETSTNPIASIFAWTRGLAHRAKLDNNKELAFFANALEEVSIETIEAGFMTKDLAACIKGL 383
           ET+T    SI     GL H A   N         A EEV+++T    F+   L    K L
Sbjct: 94  ETNTFSSTSIAQADYGLEHLAYRMNMCSAGVARKAAEEVTLQTGIKRFVAGALGPTNKTL 153

Query: 384 ---PNVQRSDYLN-TFE 396
              P+V+R DY N TF+
Sbjct: 154 SVSPSVERPDYRNITFD 170


>gi|238908505 DNA-directed RNA polymerase III B isoform 2 [Homo
           sapiens]
          Length = 1075

 Score = 29.3 bits (64), Expect = 6.9
 Identities = 21/84 (25%), Positives = 36/84 (42%), Gaps = 8/84 (9%)

Query: 215 ILKKYDGRFKDIFQEIYDKQYKSQFEAQKIWYEHRLIDDMVAQAMKSEGGFIWACKNY-- 272
           + KK++   K I  ++  KQ  +QF+  K   + ++ + MV           W+ K +  
Sbjct: 318 LFKKFNSEMKKIADQVIPKQRAAQFDVVKHMRQDQITNGMVNAISTGN----WSLKRFKM 373

Query: 273 --DGDVQSDSVAQGYGSLGMMTSV 294
              G  Q  S      +LGMMT +
Sbjct: 374 DRQGVTQVLSRLSYISALGMMTRI 397


>gi|238908503 DNA-directed RNA polymerase III B isoform 1 [Homo
           sapiens]
          Length = 1133

 Score = 29.3 bits (64), Expect = 6.9
 Identities = 21/84 (25%), Positives = 36/84 (42%), Gaps = 8/84 (9%)

Query: 215 ILKKYDGRFKDIFQEIYDKQYKSQFEAQKIWYEHRLIDDMVAQAMKSEGGFIWACKNY-- 272
           + KK++   K I  ++  KQ  +QF+  K   + ++ + MV           W+ K +  
Sbjct: 376 LFKKFNSEMKKIADQVIPKQRAAQFDVVKHMRQDQITNGMVNAISTGN----WSLKRFKM 431

Query: 273 --DGDVQSDSVAQGYGSLGMMTSV 294
              G  Q  S      +LGMMT +
Sbjct: 432 DRQGVTQVLSRLSYISALGMMTRI 455


>gi|21265040 mitochondrial ribosomal protein L22 isoform a [Homo
           sapiens]
          Length = 206

 Score = 28.9 bits (63), Expect = 9.0
 Identities = 23/88 (26%), Positives = 41/88 (46%), Gaps = 12/88 (13%)

Query: 169 VHNFEEGGGVAMGMYNQDKSIEDFAHSSFQMALSKGWPLYLSTKNTIL--KKYDGRFKDI 226
           +HN    G +A+G+  Q      + H+S  + +S+ W      KN I+   +  G  +  
Sbjct: 14  IHNLRSRGKLALGVLPQS-----YIHTSASLDISRKW----EKKNKIVYPPQLPGEPRRP 64

Query: 227 FQEIYDKQYKSQFEAQKIWYEHRLIDDM 254
             EIY  + + ++   K+WY  +LI  M
Sbjct: 65  -AEIYHCRRQIKYSKDKMWYLAKLIRGM 91


  Database: hs.faa
    Posted date:  Aug 4, 2009  4:42 PM
  Number of letters in database: 18,247,518
  Number of sequences in database:  37,866
  
Lambda     K      H
   0.318    0.134    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,113,883
Number of Sequences: 37866
Number of extensions: 640948
Number of successful extensions: 1353
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 1348
Number of HSP's gapped (non-prelim): 8
length of query: 414
length of database: 18,247,518
effective HSP length: 105
effective length of query: 309
effective length of database: 14,271,588
effective search space: 4409920692
effective search space used: 4409920692
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 63 (28.9 bits)
Search results were obtained with NCBI BLAST and RefSeq entries.