Guide to the Human Genome
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Search of human proteins with 5032143

BLASTP 2.2.11 [Jun-05-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|5032143 TBP-associated factor 1A isoform 1 [Homo sapiens]
         (450 letters)

Database: hs.faa 
           37,866 sequences; 18,247,518 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|5032143 TBP-associated factor 1A isoform 1 [Homo sapiens]          928   0.0  
gi|21536362 TBP-associated factor 1A isoform 2 [Homo sapiens]         696   0.0  
gi|31083053 ceroid-lipofuscinosis, neuronal 8 [Homo sapiens]           30   4.5  
gi|190014625 DIS3 mitotic control isoform b [Homo sapiens]             30   4.5  
gi|190014623 DIS3 mitotic control isoform a [Homo sapiens]             30   4.5  
gi|19718759 myoferlin isoform b [Homo sapiens]                         29   7.6  
gi|7305053 myoferlin isoform a [Homo sapiens]                          29   7.6  
gi|186928850 mitochondrial ribosomal protein S27 [Homo sapiens]        29   7.6  

>gi|5032143 TBP-associated factor 1A isoform 1 [Homo sapiens]
          Length = 450

 Score =  928 bits (2398), Expect = 0.0
 Identities = 450/450 (100%), Positives = 450/450 (100%)

Query: 1   MSDFSEELKGPVTDDEEVETSVLSGAGMHFPWLQTYVETVAIGGKRRKDFAQTTSACLSF 60
           MSDFSEELKGPVTDDEEVETSVLSGAGMHFPWLQTYVETVAIGGKRRKDFAQTTSACLSF
Sbjct: 1   MSDFSEELKGPVTDDEEVETSVLSGAGMHFPWLQTYVETVAIGGKRRKDFAQTTSACLSF 60

Query: 61  IQEALLKHQWQQAAEYMYSYFQTLEDSDSYKRQAAPEIIWKLGSEILFYHPKSNMESFNT 120
           IQEALLKHQWQQAAEYMYSYFQTLEDSDSYKRQAAPEIIWKLGSEILFYHPKSNMESFNT
Sbjct: 61  IQEALLKHQWQQAAEYMYSYFQTLEDSDSYKRQAAPEIIWKLGSEILFYHPKSNMESFNT 120

Query: 121 FANRMKNIGVMNYLKISLQHALYLLHHGMLKDAKRNLSEAETWRHGENTSSREILINLIQ 180
           FANRMKNIGVMNYLKISLQHALYLLHHGMLKDAKRNLSEAETWRHGENTSSREILINLIQ
Sbjct: 121 FANRMKNIGVMNYLKISLQHALYLLHHGMLKDAKRNLSEAETWRHGENTSSREILINLIQ 180

Query: 181 AYKGLLQYYTWSEKKMELSKLDKDDYAYNAVAQDVFNHSWKTSANISALIKIPGVWDPFV 240
           AYKGLLQYYTWSEKKMELSKLDKDDYAYNAVAQDVFNHSWKTSANISALIKIPGVWDPFV
Sbjct: 181 AYKGLLQYYTWSEKKMELSKLDKDDYAYNAVAQDVFNHSWKTSANISALIKIPGVWDPFV 240

Query: 241 KSYVEMLEFYGDRDGAQEVLTNYAYDEKFPSNPNAHIYLYNFLKRQKAPRSKLISVLKIL 300
           KSYVEMLEFYGDRDGAQEVLTNYAYDEKFPSNPNAHIYLYNFLKRQKAPRSKLISVLKIL
Sbjct: 241 KSYVEMLEFYGDRDGAQEVLTNYAYDEKFPSNPNAHIYLYNFLKRQKAPRSKLISVLKIL 300

Query: 301 YQIVPSHKLMLEFHTLLRKSEKEEHRKLGLEVLFGVLDFAGCTKNITAWKYLAKYLKNIL 360
           YQIVPSHKLMLEFHTLLRKSEKEEHRKLGLEVLFGVLDFAGCTKNITAWKYLAKYLKNIL
Sbjct: 301 YQIVPSHKLMLEFHTLLRKSEKEEHRKLGLEVLFGVLDFAGCTKNITAWKYLAKYLKNIL 360

Query: 361 MGNHLAWVQEEWNSRKNWWPGFHFSYFWAKSDWKEDTALACEKAFVAGLLLGKGCRYFRY 420
           MGNHLAWVQEEWNSRKNWWPGFHFSYFWAKSDWKEDTALACEKAFVAGLLLGKGCRYFRY
Sbjct: 361 MGNHLAWVQEEWNSRKNWWPGFHFSYFWAKSDWKEDTALACEKAFVAGLLLGKGCRYFRY 420

Query: 421 ILKQDHQILGKKIKRMKRSVKKYSIVNPRL 450
           ILKQDHQILGKKIKRMKRSVKKYSIVNPRL
Sbjct: 421 ILKQDHQILGKKIKRMKRSVKKYSIVNPRL 450


>gi|21536362 TBP-associated factor 1A isoform 2 [Homo sapiens]
          Length = 336

 Score =  696 bits (1797), Expect = 0.0
 Identities = 336/336 (100%), Positives = 336/336 (100%)

Query: 115 MESFNTFANRMKNIGVMNYLKISLQHALYLLHHGMLKDAKRNLSEAETWRHGENTSSREI 174
           MESFNTFANRMKNIGVMNYLKISLQHALYLLHHGMLKDAKRNLSEAETWRHGENTSSREI
Sbjct: 1   MESFNTFANRMKNIGVMNYLKISLQHALYLLHHGMLKDAKRNLSEAETWRHGENTSSREI 60

Query: 175 LINLIQAYKGLLQYYTWSEKKMELSKLDKDDYAYNAVAQDVFNHSWKTSANISALIKIPG 234
           LINLIQAYKGLLQYYTWSEKKMELSKLDKDDYAYNAVAQDVFNHSWKTSANISALIKIPG
Sbjct: 61  LINLIQAYKGLLQYYTWSEKKMELSKLDKDDYAYNAVAQDVFNHSWKTSANISALIKIPG 120

Query: 235 VWDPFVKSYVEMLEFYGDRDGAQEVLTNYAYDEKFPSNPNAHIYLYNFLKRQKAPRSKLI 294
           VWDPFVKSYVEMLEFYGDRDGAQEVLTNYAYDEKFPSNPNAHIYLYNFLKRQKAPRSKLI
Sbjct: 121 VWDPFVKSYVEMLEFYGDRDGAQEVLTNYAYDEKFPSNPNAHIYLYNFLKRQKAPRSKLI 180

Query: 295 SVLKILYQIVPSHKLMLEFHTLLRKSEKEEHRKLGLEVLFGVLDFAGCTKNITAWKYLAK 354
           SVLKILYQIVPSHKLMLEFHTLLRKSEKEEHRKLGLEVLFGVLDFAGCTKNITAWKYLAK
Sbjct: 181 SVLKILYQIVPSHKLMLEFHTLLRKSEKEEHRKLGLEVLFGVLDFAGCTKNITAWKYLAK 240

Query: 355 YLKNILMGNHLAWVQEEWNSRKNWWPGFHFSYFWAKSDWKEDTALACEKAFVAGLLLGKG 414
           YLKNILMGNHLAWVQEEWNSRKNWWPGFHFSYFWAKSDWKEDTALACEKAFVAGLLLGKG
Sbjct: 241 YLKNILMGNHLAWVQEEWNSRKNWWPGFHFSYFWAKSDWKEDTALACEKAFVAGLLLGKG 300

Query: 415 CRYFRYILKQDHQILGKKIKRMKRSVKKYSIVNPRL 450
           CRYFRYILKQDHQILGKKIKRMKRSVKKYSIVNPRL
Sbjct: 301 CRYFRYILKQDHQILGKKIKRMKRSVKKYSIVNPRL 336


>gi|31083053 ceroid-lipofuscinosis, neuronal 8 [Homo sapiens]
          Length = 286

 Score = 30.0 bits (66), Expect = 4.5
 Identities = 19/61 (31%), Positives = 25/61 (40%), Gaps = 5/61 (8%)

Query: 333 LFGVLDFAGCTKNITAWKYLAKYLKNILMGNHLAWVQEEWNSRKNWWPGFHFSYFWAKSD 392
           LF  L F GC  N+ A  YLA  +  +L+     +    W   K  W     S FW  + 
Sbjct: 140 LFAFLGFLGCLVNLQAGHYLA--MTTLLLEMSTPFTCVSWMLLKAGWSE---SLFWKLNQ 194

Query: 393 W 393
           W
Sbjct: 195 W 195


>gi|190014625 DIS3 mitotic control isoform b [Homo sapiens]
          Length = 928

 Score = 30.0 bits (66), Expect = 4.5
 Identities = 13/46 (28%), Positives = 26/46 (56%), Gaps = 2/46 (4%)

Query: 131 MNYLKISLQHALYLLHHGMLKDAKRNLSEAETWRHGENTSSREILI 176
           ++ L+  ++   YL   G  + ++ N  EA  W HG+N  ++EI++
Sbjct: 204 LSKLQQGIKSGTYL--QGTFRASRENYLEATVWIHGDNEENKEIIL 247


>gi|190014623 DIS3 mitotic control isoform a [Homo sapiens]
          Length = 958

 Score = 30.0 bits (66), Expect = 4.5
 Identities = 13/46 (28%), Positives = 26/46 (56%), Gaps = 2/46 (4%)

Query: 131 MNYLKISLQHALYLLHHGMLKDAKRNLSEAETWRHGENTSSREILI 176
           ++ L+  ++   YL   G  + ++ N  EA  W HG+N  ++EI++
Sbjct: 234 LSKLQQGIKSGTYL--QGTFRASRENYLEATVWIHGDNEENKEIIL 277


>gi|19718759 myoferlin isoform b [Homo sapiens]
          Length = 2048

 Score = 29.3 bits (64), Expect = 7.6
 Identities = 13/49 (26%), Positives = 26/49 (53%)

Query: 45   KRRKDFAQTTSACLSFIQEALLKHQWQQAAEYMYSYFQTLEDSDSYKRQ 93
            KR+KD  QT S+    ++E   +  W+ A+   + +      SD+++R+
Sbjct: 1013 KRKKDLTQTASSTARAMEELQDQEGWEYASLIGWKFHWKQRSSDTFRRR 1061


>gi|7305053 myoferlin isoform a [Homo sapiens]
          Length = 2061

 Score = 29.3 bits (64), Expect = 7.6
 Identities = 13/49 (26%), Positives = 26/49 (53%)

Query: 45   KRRKDFAQTTSACLSFIQEALLKHQWQQAAEYMYSYFQTLEDSDSYKRQ 93
            KR+KD  QT S+    ++E   +  W+ A+   + +      SD+++R+
Sbjct: 1026 KRKKDLTQTASSTARAMEELQDQEGWEYASLIGWKFHWKQRSSDTFRRR 1074


>gi|186928850 mitochondrial ribosomal protein S27 [Homo sapiens]
          Length = 414

 Score = 29.3 bits (64), Expect = 7.6
 Identities = 26/102 (25%), Positives = 49/102 (48%), Gaps = 9/102 (8%)

Query: 240 VKSYVEMLEFYGDRDGAQEVLTNYAYDEKFPSNPNAHIYLYNFLKRQKAPRSKLISVLKI 299
           + +++     Y  +D A   L N      FP N   ++ + +F+K++   +  L  V ++
Sbjct: 109 IHTWIRQCLKYDAQDKALYTLVNKVQYGIFPDNFTFNLLMDSFIKKENY-KDALSVVFEV 167

Query: 300 LYQ---IVPSHKLM---LEFHTLLRKSE--KEEHRKLGLEVL 333
           + Q    VPS +L+   + FH L +K++   EE R  G  +L
Sbjct: 168 MMQEAFEVPSTQLLSLYVLFHCLAKKTDFSWEEERNFGASLL 209


  Database: hs.faa
    Posted date:  Aug 4, 2009  4:42 PM
  Number of letters in database: 18,247,518
  Number of sequences in database:  37,866
  
Lambda     K      H
   0.320    0.134    0.413 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,883,671
Number of Sequences: 37866
Number of extensions: 712826
Number of successful extensions: 1557
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 1547
Number of HSP's gapped (non-prelim): 12
length of query: 450
length of database: 18,247,518
effective HSP length: 106
effective length of query: 344
effective length of database: 14,233,722
effective search space: 4896400368
effective search space used: 4896400368
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 63 (28.9 bits)

Search results were obtained with NCBI BLAST and RefSeq entries.


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Guide to the Human Genome
Copyright © 2010 by Stewart Scherer. All rights reserved.

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