BLASTP 2.2.11 [Jun-05-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|4827008 solute carrier family 15 (oligopeptide transporter), member 1 [Homo sapiens] (708 letters) Database: hs.faa 37,866 sequences; 18,247,518 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|4827008 solute carrier family 15 (oligopeptide transporter), ... 1414 0.0 gi|226371746 peptide transporter 2 isoform a [Homo sapiens] 636 0.0 gi|226371748 peptide transporter 2 isoform b [Homo sapiens] 603 e-172 gi|7706117 solute carrier family 15, member 3 [Homo sapiens] 107 5e-23 gi|21717816 solute carrier family 15, member 4 [Homo sapiens] 105 2e-22 >gi|4827008 solute carrier family 15 (oligopeptide transporter), member 1 [Homo sapiens] Length = 708 Score = 1414 bits (3661), Expect = 0.0 Identities = 708/708 (100%), Positives = 708/708 (100%) Query: 1 MGMSKSHSFFGYPLSIFFIVVNEFCERFSYYGMRAILILYFTNFISWDDNLSTAIYHTFV 60 MGMSKSHSFFGYPLSIFFIVVNEFCERFSYYGMRAILILYFTNFISWDDNLSTAIYHTFV Sbjct: 1 MGMSKSHSFFGYPLSIFFIVVNEFCERFSYYGMRAILILYFTNFISWDDNLSTAIYHTFV 60 Query: 61 ALCYLTPILGALIADSWLGKFKTIVSLSIVYTIGQAVTSVSSINDLTDHNHDGTPDSLPV 120 ALCYLTPILGALIADSWLGKFKTIVSLSIVYTIGQAVTSVSSINDLTDHNHDGTPDSLPV Sbjct: 61 ALCYLTPILGALIADSWLGKFKTIVSLSIVYTIGQAVTSVSSINDLTDHNHDGTPDSLPV 120 Query: 121 HVVLSLIGLALIALGTGGIKPCVSAFGGDQFEEGQEKQRNRFFSIFYLAINAGSLLSTII 180 HVVLSLIGLALIALGTGGIKPCVSAFGGDQFEEGQEKQRNRFFSIFYLAINAGSLLSTII Sbjct: 121 HVVLSLIGLALIALGTGGIKPCVSAFGGDQFEEGQEKQRNRFFSIFYLAINAGSLLSTII 180 Query: 181 TPMLRVQQCGIHSKQACYPLAFGVPAALMAVALIVFVLGSGMYKKFKPQGNIMGKVAKCI 240 TPMLRVQQCGIHSKQACYPLAFGVPAALMAVALIVFVLGSGMYKKFKPQGNIMGKVAKCI Sbjct: 181 TPMLRVQQCGIHSKQACYPLAFGVPAALMAVALIVFVLGSGMYKKFKPQGNIMGKVAKCI 240 Query: 241 GFAIKNRFRHRSKAFPKREHWLDWAKEKYDERLISQIKMVTRVMFLYIPLPMFWALFDQQ 300 GFAIKNRFRHRSKAFPKREHWLDWAKEKYDERLISQIKMVTRVMFLYIPLPMFWALFDQQ Sbjct: 241 GFAIKNRFRHRSKAFPKREHWLDWAKEKYDERLISQIKMVTRVMFLYIPLPMFWALFDQQ 300 Query: 301 GSRWTLQATTMSGKIGALEIQPDQMQTVNAILIVIMVPIFDAVLYPLIAKCGFNFTSLKK 360 GSRWTLQATTMSGKIGALEIQPDQMQTVNAILIVIMVPIFDAVLYPLIAKCGFNFTSLKK Sbjct: 301 GSRWTLQATTMSGKIGALEIQPDQMQTVNAILIVIMVPIFDAVLYPLIAKCGFNFTSLKK 360 Query: 361 MAVGMVLASMAFVVAAIVQVEIDKTLPVFPKGNEVQIKVLNIGNNTMNISLPGEMVTLGP 420 MAVGMVLASMAFVVAAIVQVEIDKTLPVFPKGNEVQIKVLNIGNNTMNISLPGEMVTLGP Sbjct: 361 MAVGMVLASMAFVVAAIVQVEIDKTLPVFPKGNEVQIKVLNIGNNTMNISLPGEMVTLGP 420 Query: 421 MSQTNAFMTFDVNKLTRINISSPGSPVTAVTDDFKQGQRHTLLVWAPNHYQVVKDGLNQK 480 MSQTNAFMTFDVNKLTRINISSPGSPVTAVTDDFKQGQRHTLLVWAPNHYQVVKDGLNQK Sbjct: 421 MSQTNAFMTFDVNKLTRINISSPGSPVTAVTDDFKQGQRHTLLVWAPNHYQVVKDGLNQK 480 Query: 481 PEKGENGIRFVNTFNELITITMSGKVYANISSYNASTYQFFPSGIKGFTISSTEIPPQCQ 540 PEKGENGIRFVNTFNELITITMSGKVYANISSYNASTYQFFPSGIKGFTISSTEIPPQCQ Sbjct: 481 PEKGENGIRFVNTFNELITITMSGKVYANISSYNASTYQFFPSGIKGFTISSTEIPPQCQ 540 Query: 541 PNFNTFYLEFGSAYTYIVQRKNDSCPEVKVFEDISANTVNMALQIPQYFLLTCGEVVFSV 600 PNFNTFYLEFGSAYTYIVQRKNDSCPEVKVFEDISANTVNMALQIPQYFLLTCGEVVFSV Sbjct: 541 PNFNTFYLEFGSAYTYIVQRKNDSCPEVKVFEDISANTVNMALQIPQYFLLTCGEVVFSV 600 Query: 601 TGLEFSYSQAPSNMKSVLQAGWLLTVAVGNIIVLIVAGAGQFSKQWAEYILFAALLLVVC 660 TGLEFSYSQAPSNMKSVLQAGWLLTVAVGNIIVLIVAGAGQFSKQWAEYILFAALLLVVC Sbjct: 601 TGLEFSYSQAPSNMKSVLQAGWLLTVAVGNIIVLIVAGAGQFSKQWAEYILFAALLLVVC 660 Query: 661 VIFAIMARFYTYINPAEIEAQFDEDEKKNRLEKSNPYFMSGANSQKQM 708 VIFAIMARFYTYINPAEIEAQFDEDEKKNRLEKSNPYFMSGANSQKQM Sbjct: 661 VIFAIMARFYTYINPAEIEAQFDEDEKKNRLEKSNPYFMSGANSQKQM 708 >gi|226371746 peptide transporter 2 isoform a [Homo sapiens] Length = 729 Score = 636 bits (1640), Expect = 0.0 Identities = 345/699 (49%), Positives = 460/699 (65%), Gaps = 56/699 (8%) Query: 12 YPLSIFFIVVNEFCERFSYYGMRAILILYFTNFISWDDNLSTAIYHTFVALCYLTPILGA 71 YPLSI FIVVNEFCERFSYYGM+A+LILYF F+ W+++ ST+IYH F +LCY TPILGA Sbjct: 42 YPLSIAFIVVNEFCERFSYYGMKAVLILYFLYFLHWNEDTSTSIYHAFSSLCYFTPILGA 101 Query: 72 LIADSWLGKFKTIVSLSIVYTIGQAVTSVSSINDLTDHNHDGTPDSLPVHVVLSLIGLAL 131 IADSWLGKFKTI+ LS+VY +G + S+ ++ L VH VLSLIGL+L Sbjct: 102 AIADSWLGKFKTIIYLSLVYVLGHVIKSLGALPILGGQ---------VVHTVLSLIGLSL 152 Query: 132 IALGTGGIKPCVSAFGGDQFEEGQEKQRNRFFSIFYLAINAGSLLSTIITPMLR--VQQC 189 IALGTGGIKPCV+AFGGDQFEE ++R R+FS+FYL+INAGSL+ST ITPMLR VQ Sbjct: 153 IALGTGGIKPCVAAFGGDQFEEKHAEERTRYFSVFYLSINAGSLISTFITPMLRGDVQCF 212 Query: 190 GIHSKQACYPLAFGVPAALMAVALIVFVLGSGMYKKFKPQGNIMGKVAKCIGFAIKNRFR 249 G + CY LAFGVP LM +AL+VF +GS +Y K P+GNI+ +V KCI FAI NRF+ Sbjct: 213 G----EDCYALAFGVPGLLMVIALVVFAMGSKIYNKPPPEGNIVAQVFKCIWFAISNRFK 268 Query: 250 HRSKAFPKREHWLDWAKEKYDERLISQIKMVTRVMFLYIPLPMFWALFDQQGSRWTLQAT 309 +RS PKR+HWLDWA EKY ++LI +K +TRV+FLYIPLPMFWAL DQQGSRWTLQA Sbjct: 269 NRSGDIPKRQHWLDWAAEKYPKQLIMDVKALTRVLFLYIPLPMFWALLDQQGSRWTLQAI 328 Query: 310 TMSGKIGALEIQPDQMQTVNAILIVIMVPIFDAVLYPLIAKCGFNFTSLKKMAVGMVLAS 369 M+ +G +QPDQMQ +N +L++I +P+FD V+Y L++KCG NF+SL+KMAVGM+LA Sbjct: 329 RMNRNLGFFVLQPDQMQVLNPLLVLIFIPLFDFVIYRLVSKCGINFSSLRKMAVGMILAC 388 Query: 370 MAFVVAAIVQVEIDKTLPVFPKGNEVQIKVLNIGNNTMNISLPGEMVTLGPMSQTNAFMT 429 +AF VAA V+++I++ P P EV ++VLN+ ++ + +++ G ++ N+ + Sbjct: 389 LAFAVAAAVEIKINEMAPAQPGPQEVFLQVLNLADDEVKVTVVG--------NENNSLLI 440 Query: 430 FDVNKLTRINISSPGSPVTAVTDDFKQGQRHTLLVWAPNHYQ------------------ 471 + + S T D + H L ++ + Q Sbjct: 441 ESIKSFQKTPHYSKLHLKTKSQDFHFHLKYHNLSLYTEHSVQEKNWYSLVIREDGNSISS 500 Query: 472 -VVKDGLNQKPEKGENGIRFVNTFNELITITMSGKVYANI-SSYNASTYQFFPSGIKGFT 529 +VKD + G +RFVNT ++ + I++S N+ Y S Y+ G Sbjct: 501 MMVKD-TESRTTNGMTTVRFVNTLHKDVNISLSTDTSLNVGEDYGVSAYRTVQRGEYPAV 559 Query: 530 ISSTEIPPQCQPNF--NTFYLEFGSAYTYIVQRKNDSCPEVKVFEDISANTVNMALQIPQ 587 TE NF N L+FG+AY +++ + + EDI AN +++A Q+PQ Sbjct: 560 HCRTE-----DKNFSLNLGLLDFGAAYLFVITNNTNQGLQAWKIEDIPANKMSIAWQLPQ 614 Query: 588 YFLLTCGEVVFSVTGLEFSYSQAPSNMKSVLQAGWLLTVAVGNIIVLIVAGAGQFS--KQ 645 Y L+T GEV+FSVTGLEFSYSQAPS+MKSVLQA WLLT+AVGNIIVL+VA QFS Q Sbjct: 615 YALVTAGEVMFSVTGLEFSYSQAPSSMKSVLQAAWLLTIAVGNIIVLVVA---QFSGLVQ 671 Query: 646 WAEYILFAALLLVVCVIFAIMARFYTYINPAEIEAQFDE 684 WAE+ILF+ LLLV+C+IF+IM +Y + ++ D+ Sbjct: 672 WAEFILFSCLLLVICLIFSIMGYYYVPVKTEDMRGPADK 710 >gi|226371748 peptide transporter 2 isoform b [Homo sapiens] Length = 698 Score = 603 bits (1554), Expect = e-172 Identities = 334/699 (47%), Positives = 442/699 (63%), Gaps = 87/699 (12%) Query: 12 YPLSIFFIVVNEFCERFSYYGMRAILILYFTNFISWDDNLSTAIYHTFVALCYLTPILGA 71 YPLSI FIVVNEFCERFSYYGM+A+LILYF F+ W+++ ST+IYH F +LCY TPILGA Sbjct: 42 YPLSIAFIVVNEFCERFSYYGMKAVLILYFLYFLHWNEDTSTSIYHAFSSLCYFTPILGA 101 Query: 72 LIADSWLGKFKTIVSLSIVYTIGQAVTSVSSINDLTDHNHDGTPDSLPVHVVLSLIGLAL 131 IADSWLGKFK VLSLIGL+L Sbjct: 102 AIADSWLGKFK----------------------------------------VLSLIGLSL 121 Query: 132 IALGTGGIKPCVSAFGGDQFEEGQEKQRNRFFSIFYLAINAGSLLSTIITPMLR--VQQC 189 IALGTGGIKPCV+AFGGDQFEE ++R R+FS+FYL+INAGSL+ST ITPMLR VQ Sbjct: 122 IALGTGGIKPCVAAFGGDQFEEKHAEERTRYFSVFYLSINAGSLISTFITPMLRGDVQCF 181 Query: 190 GIHSKQACYPLAFGVPAALMAVALIVFVLGSGMYKKFKPQGNIMGKVAKCIGFAIKNRFR 249 G + CY LAFGVP LM +AL+VF +GS +Y K P+GNI+ +V KCI FAI NRF+ Sbjct: 182 G----EDCYALAFGVPGLLMVIALVVFAMGSKIYNKPPPEGNIVAQVFKCIWFAISNRFK 237 Query: 250 HRSKAFPKREHWLDWAKEKYDERLISQIKMVTRVMFLYIPLPMFWALFDQQGSRWTLQAT 309 +RS PKR+HWLDWA EKY ++LI +K +TRV+FLYIPLPMFWAL DQQGSRWTLQA Sbjct: 238 NRSGDIPKRQHWLDWAAEKYPKQLIMDVKALTRVLFLYIPLPMFWALLDQQGSRWTLQAI 297 Query: 310 TMSGKIGALEIQPDQMQTVNAILIVIMVPIFDAVLYPLIAKCGFNFTSLKKMAVGMVLAS 369 M+ +G +QPDQMQ +N +L++I +P+FD V+Y L++KCG NF+SL+KMAVGM+LA Sbjct: 298 RMNRNLGFFVLQPDQMQVLNPLLVLIFIPLFDFVIYRLVSKCGINFSSLRKMAVGMILAC 357 Query: 370 MAFVVAAIVQVEIDKTLPVFPKGNEVQIKVLNIGNNTMNISLPGEMVTLGPMSQTNAFMT 429 +AF VAA V+++I++ P P EV ++VLN+ ++ + +++ G ++ N+ + Sbjct: 358 LAFAVAAAVEIKINEMAPAQPGPQEVFLQVLNLADDEVKVTVVG--------NENNSLLI 409 Query: 430 FDVNKLTRINISSPGSPVTAVTDDFKQGQRHTLLVWAPNHYQ------------------ 471 + + S T D + H L ++ + Q Sbjct: 410 ESIKSFQKTPHYSKLHLKTKSQDFHFHLKYHNLSLYTEHSVQEKNWYSLVIREDGNSISS 469 Query: 472 -VVKDGLNQKPEKGENGIRFVNTFNELITITMSGKVYANI-SSYNASTYQFFPSGIKGFT 529 +VKD + G +RFVNT ++ + I++S N+ Y S Y+ G Sbjct: 470 MMVKD-TESRTTNGMTTVRFVNTLHKDVNISLSTDTSLNVGEDYGVSAYRTVQRGEYPAV 528 Query: 530 ISSTEIPPQCQPNF--NTFYLEFGSAYTYIVQRKNDSCPEVKVFEDISANTVNMALQIPQ 587 TE NF N L+FG+AY +++ + + EDI AN +++A Q+PQ Sbjct: 529 HCRTE-----DKNFSLNLGLLDFGAAYLFVITNNTNQGLQAWKIEDIPANKMSIAWQLPQ 583 Query: 588 YFLLTCGEVVFSVTGLEFSYSQAPSNMKSVLQAGWLLTVAVGNIIVLIVAGAGQFS--KQ 645 Y L+T GEV+FSVTGLEFSYSQAPS+MKSVLQA WLLT+AVGNIIVL+VA QFS Q Sbjct: 584 YALVTAGEVMFSVTGLEFSYSQAPSSMKSVLQAAWLLTIAVGNIIVLVVA---QFSGLVQ 640 Query: 646 WAEYILFAALLLVVCVIFAIMARFYTYINPAEIEAQFDE 684 WAE+ILF+ LLLV+C+IF+IM +Y + ++ D+ Sbjct: 641 WAEFILFSCLLLVICLIFSIMGYYYVPVKTEDMRGPADK 679 >gi|7706117 solute carrier family 15, member 3 [Homo sapiens] Length = 581 Score = 107 bits (266), Expect = 5e-23 Identities = 84/408 (20%), Positives = 182/408 (44%), Gaps = 59/408 (14%) Query: 19 IVVNEFCERFSYYGMRAILILYF--TNFISWDDNLSTAIYHTFVALCYLTPILGALIADS 76 +++ E ER +++G+ A L+LY TNF +W +T F+ YL +G +AD Sbjct: 38 VLLVEMLERAAFFGVTANLVLYLNSTNF-NWTGEQATRAALVFLGASYLLAPVGGWLADV 96 Query: 77 WLGKFKTIVSLSIVYTIGQAVTSVSSINDLTDH--------------NHDGTPDSLPVHV 122 +LG+++ + ++Y + ++ D G P S P Sbjct: 97 YLGRYRAVALSLLLYLAASGLLPATAFPDGRSSFCGEMPASPLGPACPSAGCPRSSPSPY 156 Query: 123 VLSLI--GLALIALGTGGIKPCVSAFGGDQFEEGQEKQRNRFFSIFYLAINAGSLLSTII 180 ++ GL L+ L ++ +++FG DQ + RFF+ FY +IN G++LS ++ Sbjct: 157 CAPVLYAGLLLLGLAASSVRSNLTSFGADQVMDLGRDATRRFFNWFYWSINLGAVLSLLV 216 Query: 181 TPMLRVQQCGIHSKQACYPLAFGVPAALMAVALIVFVLGSGMYKKFKPQGNIMGKVAKCI 240 ++ + + L + +P + +A +F+ + ++ P G+ +V+ + Sbjct: 217 VAFIQ--------QNISFLLGYSIPVGCVGLAFFIFLFATPVFITKPPMGS---QVSSML 265 Query: 241 GFAIKNRFRHRSKAFPKREHWLDWAKEKYDERL----------ISQIKMVTRVMFLYIPL 290 A++N + R+ A+ DER I+ +++ +++ + + L Sbjct: 266 KLALQNCCPQLWQRHSARDR--QCARVLADERSPQPGASPQEDIANFQVLVKILPVMVTL 323 Query: 291 PMFWALFDQQGSRWTLQ-----------------ATTMSGKIGALEIQPDQMQTVNAILI 333 +W ++ Q S + LQ + + + + I + N +++ Sbjct: 324 VPYWMVYFQMQSTYVLQGLHLHIPNIFPANPANISVALRAQGSSYTIPEAWLLLANVVVV 383 Query: 334 VIMVPIFDAVLYPLIAKCGFNFTSLKKMAVGMVLASMAFVVAAIVQVE 381 +I+VP+ D ++ PL+ +C ++L+KMA+GM + +VA ++++E Sbjct: 384 LILVPLKDRLIDPLLLRCKLLPSALQKMALGMFFGFTSVIVAGVLEME 431 Score = 44.7 bits (104), Expect = 3e-04 Identities = 19/49 (38%), Positives = 31/49 (63%) Query: 584 QIPQYFLLTCGEVVFSVTGLEFSYSQAPSNMKSVLQAGWLLTVAVGNII 632 QIPQY L+ E+ S+ GLEF+YS+AP +M+ + + VG+++ Sbjct: 461 QIPQYLLIGISEIFASIPGLEFAYSEAPRSMQGAIMGIFFCLSGVGSLL 509 >gi|21717816 solute carrier family 15, member 4 [Homo sapiens] Length = 577 Score = 105 bits (261), Expect = 2e-22 Identities = 82/403 (20%), Positives = 180/403 (44%), Gaps = 39/403 (9%) Query: 8 SFFGYPLSIFFIVVNEFCERFSYYGMRAILILYFTNF-ISWDDNLSTAIYHTFVALCYLT 66 +F G + +++ E ER ++YG+ + L+L+ W+ ++ F+ L YL Sbjct: 29 AFAGRRAACGAVLLTELLERAAFYGITSNLVLFLNGAPFCWEGAQASEALLLFMGLTYLG 88 Query: 67 PILGALIADSWLGKFKTIVSLSIVYTIGQAV----------TSVSSINDLTDHNHDGTPD 116 G +AD+ LG+ + I+ +Y +G ++ L + G Sbjct: 89 SPFGGWLADARLGRARAILLSLALYLLGMLAFPLLAAPATRAALCGSARLLNCTAPGPDA 148 Query: 117 SLPVHVVLSLIGLALIALGTGGIKPCVSAFGGDQFEEGQEKQRNRFFSIFYLAINAGSLL 176 + + GL L+ LG +K ++ FG DQ ++ + RFF+ FY +IN G++L Sbjct: 149 AARCCSPATFAGLVLVGLGVATVKANITPFGADQVKDRGPEATRRFFNWFYWSINLGAIL 208 Query: 177 STIITPMLRVQQCGIHSKQACYPLAFGVPAALMAVALIVFVLGSGMYKKFKPQGNIMGKV 236 S + + +QQ + + +P + +A +VF+ G ++ P G+ + Sbjct: 209 S--LGGIAYIQQ------NVSFVTGYAIPTVCVGLAFVVFLCGQSVFITKPPDGSAFTDM 260 Query: 237 AKCIGFAIKNRFRHRSKAF----------PKREHWLDWAKEK----YDERLISQIKMVTR 282 K + ++ ++ R + ++ D K + E + +K + + Sbjct: 261 FKILTYSCCSQKRSGERQSNGEGIGVFQQSSKQSLFDSCKMSHGGPFTEEKVEDVKALVK 320 Query: 283 VMFLYIPLPMFWALFDQQGSRWTLQATTMS-GKIGALEIQPDQMQTV-----NAILIVIM 336 ++ +++ L +W ++ Q + + LQ+ + +I + P + +A+LI+++ Sbjct: 321 IVPVFLALIPYWTVYFQMQTTYVLQSLHLRIPEISNITTTPHTLPAAWLTMFDAVLILLL 380 Query: 337 VPIFDAVLYPLIAKCGFNFTSLKKMAVGMVLASMAFVVAAIVQ 379 +P+ D ++ P++ + G +SLK++AVGM + A I++ Sbjct: 381 IPLKDKLVDPILRRHGLLPSSLKRIAVGMFFVMCSAFAAGILE 423 Score = 45.1 bits (105), Expect = 2e-04 Identities = 19/49 (38%), Positives = 30/49 (61%) Query: 584 QIPQYFLLTCGEVVFSVTGLEFSYSQAPSNMKSVLQAGWLLTVAVGNII 632 Q+PQY L+ E+ S+ GLEF+YS AP +M+S + + VG+ + Sbjct: 454 QVPQYLLIGISEIFASIAGLEFAYSAAPKSMQSAIMGLFFFFSGVGSFV 502 Database: hs.faa Posted date: Aug 4, 2009 4:42 PM Number of letters in database: 18,247,518 Number of sequences in database: 37,866 Lambda K H 0.324 0.137 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 24,554,880 Number of Sequences: 37866 Number of extensions: 1026715 Number of successful extensions: 2945 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 5 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 2920 Number of HSP's gapped (non-prelim): 9 length of query: 708 length of database: 18,247,518 effective HSP length: 109 effective length of query: 599 effective length of database: 14,120,124 effective search space: 8457954276 effective search space used: 8457954276 T: 11 A: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 66 (30.0 bits)
Search results were obtained with NCBI BLAST and RefSeq entries.
Guide to the Human Genome
Copyright © 2010 by Stewart Scherer. All rights reserved.