Search of human proteins with 72534712

Search of human proteins with 72534712

BLASTP 2.2.11 [Jun-05-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|72534712 endoplasmic reticulum-golgi intermediate
compartment 32 kDa protein [Homo sapiens]
         (290 letters)

Database: hs.faa 
           37,866 sequences; 18,247,518 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|72534712 endoplasmic reticulum-golgi intermediate compartment...   599   e-172
gi|7706278 serologically defined breast cancer antigen 84 isofor...   110   1e-24
gi|38327615 serologically defined breast cancer antigen 84 isofo...   108   4e-24
gi|50959176 PTX1 protein [Homo sapiens]                                77   1e-14

>gi|72534712 endoplasmic reticulum-golgi intermediate compartment 32
           kDa protein [Homo sapiens]
          Length = 290

 Score =  599 bits (1545), Expect = e-172
 Identities = 290/290 (100%), Positives = 290/290 (100%)

Query: 1   MPFDFRRFDIYRKVPKDLTQPTYTGAIISICCCLFILFLFLSELTGFITTEVVNELYVDD 60
           MPFDFRRFDIYRKVPKDLTQPTYTGAIISICCCLFILFLFLSELTGFITTEVVNELYVDD
Sbjct: 1   MPFDFRRFDIYRKVPKDLTQPTYTGAIISICCCLFILFLFLSELTGFITTEVVNELYVDD 60

Query: 61  PDKDSGGKIDVSLNISLPNLHCELVGLDIQDEMGRHEVGHIDNSMKIPLNNGAGCRFEGQ 120
           PDKDSGGKIDVSLNISLPNLHCELVGLDIQDEMGRHEVGHIDNSMKIPLNNGAGCRFEGQ
Sbjct: 61  PDKDSGGKIDVSLNISLPNLHCELVGLDIQDEMGRHEVGHIDNSMKIPLNNGAGCRFEGQ 120

Query: 121 FSINKVPGNFHVSTHSATAQPQNPDMTHVIHKLSFGDTLQVQNIHGAFNALGGADRLTSN 180
           FSINKVPGNFHVSTHSATAQPQNPDMTHVIHKLSFGDTLQVQNIHGAFNALGGADRLTSN
Sbjct: 121 FSINKVPGNFHVSTHSATAQPQNPDMTHVIHKLSFGDTLQVQNIHGAFNALGGADRLTSN 180

Query: 181 PLASHDYILKIVPTVYEDKSGKQRYSYQYTVANKEYVAYSHTGRIIPAIWFRYDLSPITV 240
           PLASHDYILKIVPTVYEDKSGKQRYSYQYTVANKEYVAYSHTGRIIPAIWFRYDLSPITV
Sbjct: 181 PLASHDYILKIVPTVYEDKSGKQRYSYQYTVANKEYVAYSHTGRIIPAIWFRYDLSPITV 240

Query: 241 KYTERRQPLYRFITTICAIIGGTFTVAGILDSCIFTASEAWKKIQLGKMH 290
           KYTERRQPLYRFITTICAIIGGTFTVAGILDSCIFTASEAWKKIQLGKMH
Sbjct: 241 KYTERRQPLYRFITTICAIIGGTFTVAGILDSCIFTASEAWKKIQLGKMH 290


>gi|7706278 serologically defined breast cancer antigen 84 isoform b
           [Homo sapiens]
          Length = 383

 Score =  110 bits (276), Expect = 1e-24
 Identities = 71/199 (35%), Positives = 101/199 (50%), Gaps = 19/199 (9%)

Query: 104 SMKIPLNNGAGCRFEGQFSINKVPGNFHVSTHSATAQPQ------------NPDMTHVIH 151
           S K+      GC+  G   +NKV GNFH +   +  Q              N +MTH I 
Sbjct: 188 SQKMQEQKNEGCQVYGFLEVNKVAGNFHFAPGKSFQQSHVHVHDLQSFGLDNINMTHYIQ 247

Query: 152 KLSFGDTLQVQNIHGAFNALGGADRLTSNPLASHDYILKIVPTVYEDKSGKQRYSYQYTV 211
            LSFG+     +  G  N L   +           Y +K+VPTVY    G+   + Q++V
Sbjct: 248 HLSFGE-----DYPGIVNPLDHTNVTAPQASMMFQYFVKVVPTVYMKVDGEVLRTNQFSV 302

Query: 212 ANKEYVAYSHTG-RIIPAIWFRYDLSPITVKYTERRQPLYRFITTICAIIGGTFTVAGIL 270
              E VA    G + +P ++  Y+LSP+ VK TE+ +    F+T +CAIIGG FTVAG++
Sbjct: 303 TRHEKVANGLLGDQGLPGVFVLYELSPMMVKLTEKHRSFTHFLTGVCAIIGGMFTVAGLI 362

Query: 271 DSCIFTASEA-WKKIQLGK 288
           DS I+ ++ A  KKI LGK
Sbjct: 363 DSLIYHSARAIQKKIDLGK 381



 Score = 65.9 bits (159), Expect = 4e-11
 Identities = 36/101 (35%), Positives = 56/101 (55%), Gaps = 3/101 (2%)

Query: 6   RRFDIYRKVPKDLTQPTYTGAIISICCCLFILFLFLSELTGFITTEVVNELYVDDPDKDS 65
           ++FD Y K  +D    T  GA ++I   L +L LFLSEL  ++TTEV  ELYV   DK  
Sbjct: 8   KQFDAYPKTLEDFRVKTCGGATVTIVSGLLMLLLFLSELQYYLTTEVHPELYV---DKSR 64

Query: 66  GGKIDVSLNISLPNLHCELVGLDIQDEMGRHEVGHIDNSMK 106
           G K+ +++++  P++ C  + +D  D  G  ++    N  K
Sbjct: 65  GDKLKINIDVLFPHMPCAYLSIDAMDVAGEQQLDVEHNLFK 105


>gi|38327615 serologically defined breast cancer antigen 84 isoform
           a [Homo sapiens]
          Length = 388

 Score =  108 bits (271), Expect = 4e-24
 Identities = 71/204 (34%), Positives = 101/204 (49%), Gaps = 24/204 (11%)

Query: 104 SMKIPLNNGAGCRFEGQFSINKVPGNFHVSTHSATAQPQ-----------------NPDM 146
           S K+      GC+  G   +NKV GNFH +   +  Q                   N +M
Sbjct: 188 SQKMQEQKNEGCQVYGFLEVNKVAGNFHFAPGKSFQQSHVHVHAVEIHDLQSFGLDNINM 247

Query: 147 THVIHKLSFGDTLQVQNIHGAFNALGGADRLTSNPLASHDYILKIVPTVYEDKSGKQRYS 206
           TH I  LSFG+     +  G  N L   +           Y +K+VPTVY    G+   +
Sbjct: 248 THYIQHLSFGE-----DYPGIVNPLDHTNVTAPQASMMFQYFVKVVPTVYMKVDGEVLRT 302

Query: 207 YQYTVANKEYVAYSHTG-RIIPAIWFRYDLSPITVKYTERRQPLYRFITTICAIIGGTFT 265
            Q++V   E VA    G + +P ++  Y+LSP+ VK TE+ +    F+T +CAIIGG FT
Sbjct: 303 NQFSVTRHEKVANGLLGDQGLPGVFVLYELSPMMVKLTEKHRSFTHFLTGVCAIIGGMFT 362

Query: 266 VAGILDSCIFTASEA-WKKIQLGK 288
           VAG++DS I+ ++ A  KKI LGK
Sbjct: 363 VAGLIDSLIYHSARAIQKKIDLGK 386



 Score = 65.9 bits (159), Expect = 4e-11
 Identities = 36/101 (35%), Positives = 56/101 (55%), Gaps = 3/101 (2%)

Query: 6   RRFDIYRKVPKDLTQPTYTGAIISICCCLFILFLFLSELTGFITTEVVNELYVDDPDKDS 65
           ++FD Y K  +D    T  GA ++I   L +L LFLSEL  ++TTEV  ELYV   DK  
Sbjct: 8   KQFDAYPKTLEDFRVKTCGGATVTIVSGLLMLLLFLSELQYYLTTEVHPELYV---DKSR 64

Query: 66  GGKIDVSLNISLPNLHCELVGLDIQDEMGRHEVGHIDNSMK 106
           G K+ +++++  P++ C  + +D  D  G  ++    N  K
Sbjct: 65  GDKLKINIDVLFPHMPCAYLSIDAMDVAGEQQLDVEHNLFK 105


>gi|50959176 PTX1 protein [Homo sapiens]
          Length = 377

 Score = 77.4 bits (189), Expect = 1e-14
 Identities = 47/172 (27%), Positives = 80/172 (46%), Gaps = 25/172 (14%)

Query: 115 CRFEGQFSINKVPGNFHVSTHSATAQP------------QNPDMTHVIHKLSFGDTLQVQ 162
           CR  G   +NKV GNFH++   A   P            ++ + +H I  LSFG+     
Sbjct: 170 CRIHGHLYVNKVAGNFHITVGKAIPHPRGHAHLAALVNHESYNFSHRIDHLSFGEL---- 225

Query: 163 NIHGAFNALGGADRLTSNPLASHDYILKIVPT---VYEDKSGKQRYSYQYTVANKEYVAY 219
            +    N L G +++  +      Y + +VPT    Y+  +     ++Q++V  +E +  
Sbjct: 226 -VPAIINPLDGTEKIAIDHNQMFQYFITVVPTKLHTYKISAD----THQFSVTERERIIN 280

Query: 220 SHTGRI-IPAIWFRYDLSPITVKYTERRQPLYRFITTICAIIGGTFTVAGIL 270
              G   +  I+ +YDLS + V  TE   P ++F   +C I+GG F+  G+L
Sbjct: 281 HAAGSHGVSGIFMKYDLSSLMVTVTEEHMPFWQFFVRLCGIVGGIFSTTGML 332


  Database: hs.faa
    Posted date:  Aug 4, 2009  4:42 PM
  Number of letters in database: 18,247,518
  Number of sequences in database:  37,866
  
Lambda     K      H
   0.323    0.140    0.430 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,114,569
Number of Sequences: 37866
Number of extensions: 484204
Number of successful extensions: 900
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 885
Number of HSP's gapped (non-prelim): 8
length of query: 290
length of database: 18,247,518
effective HSP length: 101
effective length of query: 189
effective length of database: 14,423,052
effective search space: 2725956828
effective search space used: 2725956828
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 61 (28.1 bits)
Search results were obtained with NCBI BLAST and RefSeq entries.