Search of human proteins with 189011546

Search of human proteins with 189011546

BLASTP 2.2.11 [Jun-05-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|189011546 N-acylsphingosine amidohydrolase 1 isoform b [Homo
sapiens]
         (411 letters)

Database: hs.faa 
           37,866 sequences; 18,247,518 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|189011546 N-acylsphingosine amidohydrolase 1 isoform b [Homo ...   852   0.0  
gi|189011550 N-acylsphingosine amidohydrolase 1 isoform c [Homo ...   776   0.0  
gi|189011548 N-acylsphingosine amidohydrolase 1 isoform a prepro...   769   0.0  
gi|109148550 N-acylethanolamine acid amidase isoform 1 precursor...   191   1e-48
gi|109148548 N-acylethanolamine acid amidase isoform 2 precursor...   170   2e-42
gi|4506203 proteasome beta 7 subunit proprotein [Homo sapiens]         29   8.9  

>gi|189011546 N-acylsphingosine amidohydrolase 1 isoform b [Homo
           sapiens]
          Length = 411

 Score =  852 bits (2201), Expect = 0.0
 Identities = 411/411 (100%), Positives = 411/411 (100%)

Query: 1   MNCCIGLGEKARGSHRASYPSLSALFTEASILGFGSFAVKAQWTEDCRKSTYPPSGPTYR 60
           MNCCIGLGEKARGSHRASYPSLSALFTEASILGFGSFAVKAQWTEDCRKSTYPPSGPTYR
Sbjct: 1   MNCCIGLGEKARGSHRASYPSLSALFTEASILGFGSFAVKAQWTEDCRKSTYPPSGPTYR 60

Query: 61  GAVPWYTINLDLPPYKRWHELMLDKAPVLKVIVNSLKNMINTFVPSGKIMQVVDEKLPGL 120
           GAVPWYTINLDLPPYKRWHELMLDKAPVLKVIVNSLKNMINTFVPSGKIMQVVDEKLPGL
Sbjct: 61  GAVPWYTINLDLPPYKRWHELMLDKAPVLKVIVNSLKNMINTFVPSGKIMQVVDEKLPGL 120

Query: 121 LGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSIVAEDKKGHLIHGRNMDFG 180
           LGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSIVAEDKKGHLIHGRNMDFG
Sbjct: 121 LGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSIVAEDKKGHLIHGRNMDFG 180

Query: 181 VFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTGFKPGLFSLTLN 240
           VFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTGFKPGLFSLTLN
Sbjct: 181 VFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTGFKPGLFSLTLN 240

Query: 241 ERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILG 300
           ERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILG
Sbjct: 241 ERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILG 300

Query: 301 GNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKMCLNRT 360
           GNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKMCLNRT
Sbjct: 301 GNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKMCLNRT 360

Query: 361 SQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKGQFETYLRDCPDPCIGW 411
           SQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKGQFETYLRDCPDPCIGW
Sbjct: 361 SQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKGQFETYLRDCPDPCIGW 411


>gi|189011550 N-acylsphingosine amidohydrolase 1 isoform c [Homo
           sapiens]
          Length = 389

 Score =  776 bits (2003), Expect = 0.0
 Identities = 382/418 (91%), Positives = 382/418 (91%), Gaps = 36/418 (8%)

Query: 1   MNCCIGLGEKARGSHRASYPSLSALFTEASILGFGSFAVKAQWTEDCRKSTYPPSGPT-- 58
           MNCCIGLGEKARGSHRASYPSLSALFTEASILGFGSFAVKAQWTEDCRKSTYPPSGPT  
Sbjct: 1   MNCCIGLGEKARGSHRASYPSLSALFTEASILGFGSFAVKAQWTEDCRKSTYPPSGPTVF 60

Query: 59  -----YRGAVPWYTINLDLPPYKRWHELMLDKAPVLKVIVNSLKNMINTFVPSGKIMQVV 113
                YRGAVPWYTINLDLPPYKRWHELMLDKAPV                         
Sbjct: 61  PAVIRYRGAVPWYTINLDLPPYKRWHELMLDKAPV------------------------- 95

Query: 114 DEKLPGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSIVAEDKKGHLIH 173
               PGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSIVAEDKKGHLIH
Sbjct: 96  ----PGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSIVAEDKKGHLIH 151

Query: 174 GRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTGFKPG 233
           GRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTGFKPG
Sbjct: 152 GRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTGFKPG 211

Query: 234 LFSLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILA 293
           LFSLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILA
Sbjct: 212 LFSLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILA 271

Query: 294 PAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPA 353
           PAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPA
Sbjct: 272 PAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPA 331

Query: 354 KMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKGQFETYLRDCPDPCIGW 411
           KMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKGQFETYLRDCPDPCIGW
Sbjct: 332 KMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKGQFETYLRDCPDPCIGW 389


>gi|189011548 N-acylsphingosine amidohydrolase 1 isoform a
           preproprotein [Homo sapiens]
          Length = 395

 Score =  769 bits (1986), Expect = 0.0
 Identities = 369/369 (100%), Positives = 369/369 (100%)

Query: 43  WTEDCRKSTYPPSGPTYRGAVPWYTINLDLPPYKRWHELMLDKAPVLKVIVNSLKNMINT 102
           WTEDCRKSTYPPSGPTYRGAVPWYTINLDLPPYKRWHELMLDKAPVLKVIVNSLKNMINT
Sbjct: 27  WTEDCRKSTYPPSGPTYRGAVPWYTINLDLPPYKRWHELMLDKAPVLKVIVNSLKNMINT 86

Query: 103 FVPSGKIMQVVDEKLPGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSI 162
           FVPSGKIMQVVDEKLPGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSI
Sbjct: 87  FVPSGKIMQVVDEKLPGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSI 146

Query: 163 VAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAG 222
           VAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAG
Sbjct: 147 VAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAG 206

Query: 223 YVGMLTGFKPGLFSLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEA 282
           YVGMLTGFKPGLFSLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEA
Sbjct: 207 YVGMLTGFKPGLFSLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEA 266

Query: 283 KNLLTKTKILAPAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKH 342
           KNLLTKTKILAPAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKH
Sbjct: 267 KNLLTKTKILAPAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKH 326

Query: 343 PFFLDDRRTPAKMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKGQFETYLR 402
           PFFLDDRRTPAKMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKGQFETYLR
Sbjct: 327 PFFLDDRRTPAKMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKGQFETYLR 386

Query: 403 DCPDPCIGW 411
           DCPDPCIGW
Sbjct: 387 DCPDPCIGW 395


>gi|109148550 N-acylethanolamine acid amidase isoform 1 precursor
           [Homo sapiens]
          Length = 359

 Score =  191 bits (484), Expect = 1e-48
 Identities = 116/352 (32%), Positives = 186/352 (52%), Gaps = 33/352 (9%)

Query: 62  AVPWYTINLDLPPYKRW------HELMLDKAPVLKVIVNSLKNMINTFVPSGKIMQVVDE 115
           A P + ++LD  P  RW      ++L L +A + +VI + +   ++  +  GK++  ++ 
Sbjct: 32  AAPRFNVSLDSVPELRWLPVLRHYDLDLVRAAMAQVIGDRVPKWVHVLI--GKVVLELER 89

Query: 116 KLPGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSIVAEDKKGHLIHGR 175
            LP        PF  E++G+    ++ L + +  N+ YE    CTSIVA+D +GH+ HGR
Sbjct: 90  FLPQ-------PFTGEIRGMCDFMNLSLADCLLVNLAYESSVFCTSIVAQDSRGHIYHGR 142

Query: 176 NMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTGFKPGLF 235
           N+D+                  L+ LTV++ F +N +  F  ++F GYVG+ TG  P  F
Sbjct: 143 NLDYP-------------FGNVLRKLTVDVQFLKNGQIAFTGTTFIGYVGLWTGQSPHKF 189

Query: 236 SLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPA 295
           +++ +ER    G +       L ++ +  + +L R  L  S ++E A   L KT ++A  
Sbjct: 190 TVSGDERDK--GWWWENAIAALFRRHIP-VSWLIRATLSESENFEAAVGKLAKTPLIADV 246

Query: 296 YFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKM 355
           Y+I+GG    EG VITR+R    D++ LD   G W+ V+TNYD WK     DDRRT A  
Sbjct: 247 YYIVGGTSPREGVVITRNRDGPADIWPLDPLNGAWFRVETNYDHWKPAPKEDDRRTSAIK 306

Query: 356 CLNRTSQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKGQFETYLRDCPDP 407
            LN T Q N+S E ++ +LS  PV N  T+YTT+  ++ G  + Y+    +P
Sbjct: 307 ALNATGQANLSLEALFQILSVVPVYNNFTIYTTV--MSAGSPDKYMTRIRNP 356


>gi|109148548 N-acylethanolamine acid amidase isoform 2 precursor
           [Homo sapiens]
          Length = 323

 Score =  170 bits (431), Expect = 2e-42
 Identities = 104/316 (32%), Positives = 166/316 (52%), Gaps = 31/316 (9%)

Query: 62  AVPWYTINLDLPPYKRW------HELMLDKAPVLKVIVNSLKNMINTFVPSGKIMQVVDE 115
           A P + ++LD  P  RW      ++L L +A + +VI + +   ++  +  GK++  ++ 
Sbjct: 32  AAPRFNVSLDSVPELRWLPVLRHYDLDLVRAAMAQVIGDRVPKWVHVLI--GKVVLELER 89

Query: 116 KLPGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSIVAEDKKGHLIHGR 175
            LP        PF  E++G+    ++ L + +  N+ YE    CTSIVA+D +GH+ HGR
Sbjct: 90  FLPQ-------PFTGEIRGMCDFMNLSLADCLLVNLAYESSVFCTSIVAQDSRGHIYHGR 142

Query: 176 NMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTGFKPGLF 235
           N+D+                  L+ LTV++ F +N +  F  ++F GYVG+ TG  P  F
Sbjct: 143 NLDYP-------------FGNVLRKLTVDVQFLKNGQIAFTGTTFIGYVGLWTGQSPHKF 189

Query: 236 SLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPA 295
           +++ +ER    G +       L ++ +  + +L R  L  S ++E A   L KT ++A  
Sbjct: 190 TVSGDERDK--GWWWENAIAALFRRHIP-VSWLIRATLSESENFEAAVGKLAKTPLIADV 246

Query: 296 YFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKM 355
           Y+I+GG    EG VITR+R    D++ LD   G W+ V+TNYD WK     DDRRT A  
Sbjct: 247 YYIVGGTSPREGVVITRNRDGPADIWPLDPLNGAWFRVETNYDHWKPAPKEDDRRTSAIK 306

Query: 356 CLNRTSQENISFETMY 371
            LN T Q N+S E ++
Sbjct: 307 ALNATGQANLSLEALF 322


>gi|4506203 proteasome beta 7 subunit proprotein [Homo sapiens]
          Length = 277

 Score = 28.9 bits (63), Expect = 8.9
 Identities = 17/49 (34%), Positives = 30/49 (61%), Gaps = 1/49 (2%)

Query: 280 EEAKNLLTKTKILAPAYFILGGNQSGEGCVITRDRKESLDVYELDAKQG 328
           EEAKNL+++  I A  +  LG   + + CVI++++ + L  Y +  K+G
Sbjct: 192 EEAKNLVSEA-IAAGIFNDLGSGSNIDLCVISKNKLDFLRPYTVPNKKG 239


  Database: hs.faa
    Posted date:  Aug 4, 2009  4:42 PM
  Number of letters in database: 18,247,518
  Number of sequences in database:  37,866
  
Lambda     K      H
   0.321    0.139    0.435 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,848,804
Number of Sequences: 37866
Number of extensions: 692722
Number of successful extensions: 1314
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1303
Number of HSP's gapped (non-prelim): 6
length of query: 411
length of database: 18,247,518
effective HSP length: 105
effective length of query: 306
effective length of database: 14,271,588
effective search space: 4367105928
effective search space used: 4367105928
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 63 (28.9 bits)
Search results were obtained with NCBI BLAST and RefSeq entries.