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Search of human proteins with 4885585
BLASTP 2.2.11 [Jun-05-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= gi|4885585 SUMO1 activating enzyme subunit 1 isoform a [Homo
sapiens]
(346 letters)
Database: hs.faa
37,866 sequences; 18,247,518 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|4885585 SUMO1 activating enzyme subunit 1 isoform a [Homo sap... 691 0.0
gi|7705473 SUMO1 activating enzyme subunit 1 isoform a [Homo sap... 684 0.0
gi|225543288 SUMO1 activating enzyme subunit 1 isoform c [Homo s... 485 e-137
gi|225543285 SUMO1 activating enzyme subunit 1 isoform b [Homo s... 485 e-137
gi|23510338 ubiquitin-activating enzyme E1 [Homo sapiens] 121 1e-27
gi|23510340 ubiquitin-activating enzyme E1 [Homo sapiens] 121 1e-27
gi|38045948 ubiquitin-like modifier activating enzyme 7 [Homo sa... 111 1e-24
gi|4502169 NEDD8 activating enzyme E1 subunit 1 isoform a [Homo ... 100 2e-21
gi|66363686 NEDD8 activating enzyme E1 subunit 1 isoform b [Homo... 91 2e-18
gi|150417996 ubiquitin-activating enzyme E1-like 2 [Homo sapiens] 87 3e-17
gi|4885649 SUMO-1 activating enzyme subunit 2 [Homo sapiens] 58 1e-08
gi|38045944 ubiquitin-activating enzyme 3 isoform 2 [Homo sapiens] 56 5e-08
gi|38045942 ubiquitin-activating enzyme 3 isoform 1 [Homo sapiens] 56 5e-08
gi|7657339 molybdenum cofactor synthesis 3 [Homo sapiens] 48 1e-05
gi|66363688 NEDD8 activating enzyme E1 subunit 1 isoform c [Homo... 48 1e-05
gi|222144229 APG7 autophagy 7-like isoform c [Homo sapiens] 37 0.026
gi|222144227 APG7 autophagy 7-like isoform b [Homo sapiens] 37 0.026
gi|5453668 APG7 autophagy 7-like isoform a [Homo sapiens] 37 0.026
gi|38327032 ubiquitin-activating enzyme 5 isoform 2 [Homo sapiens] 35 0.13
gi|13376212 ubiquitin-activating enzyme 5 isoform 1 [Homo sapiens] 35 0.13
gi|193788653 hypothetical protein LOC100131897 [Homo sapiens] 31 1.9
gi|109809739 La ribonucleoprotein domain family, member 7 [Homo ... 30 4.1
gi|109809741 La ribonucleoprotein domain family, member 7 [Homo ... 30 4.1
gi|189027141 abhydrolase domain containing 6 [Homo sapiens] 28 9.2
gi|104526627 triadin [Homo sapiens] 28 9.2
gi|40805823 collagen, type XXII, alpha 1 [Homo sapiens] 28 9.2
>gi|4885585 SUMO1 activating enzyme subunit 1 isoform a [Homo
sapiens]
Length = 346
Score = 691 bits (1784), Expect = 0.0
Identities = 346/346 (100%), Positives = 346/346 (100%)
Query: 1 MVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGV 60
MVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGV
Sbjct: 1 MVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGV 60
Query: 61 KGLTMLDHEQVTPEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDTEDIEKKPE 120
KGLTMLDHEQVTPEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDTEDIEKKPE
Sbjct: 61 KGLTMLDHEQVTPEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDTEDIEKKPE 120
Query: 121 SFFTQFDAVCLTCCSRDVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTK 180
SFFTQFDAVCLTCCSRDVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTK
Sbjct: 121 SFFTQFDAVCLTCCSRDVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTK 180
Query: 181 VAKVSQGVEDGPDTKRAKLDSSETTMVKKKVVFCPVKEALEVDWSSEKAKAALKRTTSDY 240
VAKVSQGVEDGPDTKRAKLDSSETTMVKKKVVFCPVKEALEVDWSSEKAKAALKRTTSDY
Sbjct: 181 VAKVSQGVEDGPDTKRAKLDSSETTMVKKKVVFCPVKEALEVDWSSEKAKAALKRTTSDY 240
Query: 241 FLLQVLLKFRTDKGRDPSSDTYEEDSELLLQIRNDVLDSLGISPDLLPEDFVRYCFSEMA 300
FLLQVLLKFRTDKGRDPSSDTYEEDSELLLQIRNDVLDSLGISPDLLPEDFVRYCFSEMA
Sbjct: 241 FLLQVLLKFRTDKGRDPSSDTYEEDSELLLQIRNDVLDSLGISPDLLPEDFVRYCFSEMA 300
Query: 301 PVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGMKGNGIVECLGPK 346
PVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGMKGNGIVECLGPK
Sbjct: 301 PVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGMKGNGIVECLGPK 346
>gi|7705473 SUMO1 activating enzyme subunit 1 isoform a [Homo
sapiens]
Length = 346
Score = 684 bits (1765), Expect = 0.0
Identities = 342/346 (98%), Positives = 343/346 (99%)
Query: 1 MVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGV 60
MVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGV
Sbjct: 1 MVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGV 60
Query: 61 KGLTMLDHEQVTPEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDTEDIEKKPE 120
KGLTMLDHEQVTPEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDTEDIEKKPE
Sbjct: 61 KGLTMLDHEQVTPEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDTEDIEKKPE 120
Query: 121 SFFTQFDAVCLTCCSRDVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTK 180
SFFTQFDAVCLTCCSRDVIVKVDQICHKNSIKFFTGD FGYHGYTFANLGEHEFVEE+T
Sbjct: 121 SFFTQFDAVCLTCCSRDVIVKVDQICHKNSIKFFTGDAFGYHGYTFANLGEHEFVEEETD 180
Query: 181 VAKVSQGVEDGPDTKRAKLDSSETTMVKKKVVFCPVKEALEVDWSSEKAKAALKRTTSDY 240
VAKVS GVEDGPDTKRAKLDSSETTMVKKKVVFCPVKEALEVDWSSEKAKAALKRTTSDY
Sbjct: 181 VAKVSHGVEDGPDTKRAKLDSSETTMVKKKVVFCPVKEALEVDWSSEKAKAALKRTTSDY 240
Query: 241 FLLQVLLKFRTDKGRDPSSDTYEEDSELLLQIRNDVLDSLGISPDLLPEDFVRYCFSEMA 300
FLLQVLLKFRTDKGRDPSSDTYEEDSELLLQIRNDVLDSLGISPDLLPEDFVRYCFSEMA
Sbjct: 241 FLLQVLLKFRTDKGRDPSSDTYEEDSELLLQIRNDVLDSLGISPDLLPEDFVRYCFSEMA 300
Query: 301 PVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGMKGNGIVECLGPK 346
PVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGMKGNGIVECLGPK
Sbjct: 301 PVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGMKGNGIVECLGPK 346
>gi|225543288 SUMO1 activating enzyme subunit 1 isoform c [Homo
sapiens]
Length = 266
Score = 485 bits (1248), Expect = e-137
Identities = 244/244 (100%), Positives = 244/244 (100%)
Query: 1 MVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGV 60
MVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGV
Sbjct: 1 MVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGV 60
Query: 61 KGLTMLDHEQVTPEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDTEDIEKKPE 120
KGLTMLDHEQVTPEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDTEDIEKKPE
Sbjct: 61 KGLTMLDHEQVTPEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDTEDIEKKPE 120
Query: 121 SFFTQFDAVCLTCCSRDVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTK 180
SFFTQFDAVCLTCCSRDVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTK
Sbjct: 121 SFFTQFDAVCLTCCSRDVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTK 180
Query: 181 VAKVSQGVEDGPDTKRAKLDSSETTMVKKKVVFCPVKEALEVDWSSEKAKAALKRTTSDY 240
VAKVSQGVEDGPDTKRAKLDSSETTMVKKKVVFCPVKEALEVDWSSEKAKAALKRTTSDY
Sbjct: 181 VAKVSQGVEDGPDTKRAKLDSSETTMVKKKVVFCPVKEALEVDWSSEKAKAALKRTTSDY 240
Query: 241 FLLQ 244
FLLQ
Sbjct: 241 FLLQ 244
>gi|225543285 SUMO1 activating enzyme subunit 1 isoform b [Homo
sapiens]
Length = 299
Score = 485 bits (1248), Expect = e-137
Identities = 244/244 (100%), Positives = 244/244 (100%)
Query: 1 MVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGV 60
MVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGV
Sbjct: 1 MVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGV 60
Query: 61 KGLTMLDHEQVTPEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDTEDIEKKPE 120
KGLTMLDHEQVTPEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDTEDIEKKPE
Sbjct: 61 KGLTMLDHEQVTPEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDTEDIEKKPE 120
Query: 121 SFFTQFDAVCLTCCSRDVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTK 180
SFFTQFDAVCLTCCSRDVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTK
Sbjct: 121 SFFTQFDAVCLTCCSRDVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTK 180
Query: 181 VAKVSQGVEDGPDTKRAKLDSSETTMVKKKVVFCPVKEALEVDWSSEKAKAALKRTTSDY 240
VAKVSQGVEDGPDTKRAKLDSSETTMVKKKVVFCPVKEALEVDWSSEKAKAALKRTTSDY
Sbjct: 181 VAKVSQGVEDGPDTKRAKLDSSETTMVKKKVVFCPVKEALEVDWSSEKAKAALKRTTSDY 240
Query: 241 FLLQ 244
FLLQ
Sbjct: 241 FLLQ 244
>gi|23510338 ubiquitin-activating enzyme E1 [Homo sapiens]
Length = 1058
Score = 121 bits (303), Expect = 1e-27
Identities = 105/389 (26%), Positives = 158/389 (40%), Gaps = 65/389 (16%)
Query: 9 GGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDH 68
G ++ + Y RQ+ + G EA KRL+ S VL+ GL+GLG EIAKN+IL GVK +T+ D
Sbjct: 45 GSEADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQ 104
Query: 69 EQVTPEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDTEDIEKKPESFFTQFDA 128
D +QF +R +G+NRAE S R LN V V T + E F + F
Sbjct: 105 GTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVTAYTGPLV---EDFLSGFQV 161
Query: 129 VCLTCCSRDVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEFVEEK---------- 178
V LT + ++V + CH IK D G G F + GE + +
Sbjct: 162 VVLTNTPLEDQLRVGEFCHNRGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSAMV 221
Query: 179 TKVAKVSQGVEDGPDTKRAKLDSSE----------------------------------- 203
+ V K + GV D R +S +
Sbjct: 222 SMVTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMVELNGNQPMEIKVLGPYTFSICDTS 281
Query: 204 ------------TTMVKKKVVFCPVKEALEVDWSSEKAKAALKRTTSDYFLLQVLLKFRT 251
V KK+ F + +L A R + Q L +F
Sbjct: 282 NFSDYIRGGIVSQVKVPKKISFKSLVASLAEPDFVVTDFAKFSRPAQLHIGFQALHQFCA 341
Query: 252 DKGRDPSSDTYEEDSELLLQIRNDVLDSL-GISPDLLPEDFVR----YCFSEMAPVCAVV 306
GR P E+ +EL+ + +L + + L ED +R ++AP+ A +
Sbjct: 342 QHGRPPRPRNEEDAAELVALAQAVNARALPAVQQNNLDEDLIRKLAYVAAGDLAPINAFI 401
Query: 307 GGILAQEIVKALSQRDPPHNNFFFFDGMK 335
GG+ AQE++KA S + P + +FD ++
Sbjct: 402 GGLAAQEVMKACSGKFMPIMQWLYFDALE 430
Score = 48.5 bits (114), Expect = 9e-06
Identities = 34/141 (24%), Positives = 64/141 (45%), Gaps = 14/141 (9%)
Query: 18 QYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGV-----KGLTMLDHEQVT 72
+YD Q+ ++G + Q++L + LVG +G E+ KN + G+ + + D + +
Sbjct: 450 RYDGQVAVFGSDLQEKLGKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEIIVTDMDTIE 509
Query: 73 PEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVK-------VDTEDIEKKPESFFTQ 125
+ QFL R V + +++ + + +NP + V DTE I + FF
Sbjct: 510 KSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERI--YDDDFFQN 567
Query: 126 FDAVCLTCCSRDVIVKVDQIC 146
D V + D + +D+ C
Sbjct: 568 LDGVANALDNVDARMYMDRRC 588
>gi|23510340 ubiquitin-activating enzyme E1 [Homo sapiens]
Length = 1058
Score = 121 bits (303), Expect = 1e-27
Identities = 105/389 (26%), Positives = 158/389 (40%), Gaps = 65/389 (16%)
Query: 9 GGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDH 68
G ++ + Y RQ+ + G EA KRL+ S VL+ GL+GLG EIAKN+IL GVK +T+ D
Sbjct: 45 GSEADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQ 104
Query: 69 EQVTPEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDTEDIEKKPESFFTQFDA 128
D +QF +R +G+NRAE S R LN V V T + E F + F
Sbjct: 105 GTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVTAYTGPLV---EDFLSGFQV 161
Query: 129 VCLTCCSRDVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEFVEEK---------- 178
V LT + ++V + CH IK D G G F + GE + +
Sbjct: 162 VVLTNTPLEDQLRVGEFCHNRGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSAMV 221
Query: 179 TKVAKVSQGVEDGPDTKRAKLDSSE----------------------------------- 203
+ V K + GV D R +S +
Sbjct: 222 SMVTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMVELNGNQPMEIKVLGPYTFSICDTS 281
Query: 204 ------------TTMVKKKVVFCPVKEALEVDWSSEKAKAALKRTTSDYFLLQVLLKFRT 251
V KK+ F + +L A R + Q L +F
Sbjct: 282 NFSDYIRGGIVSQVKVPKKISFKSLVASLAEPDFVVTDFAKFSRPAQLHIGFQALHQFCA 341
Query: 252 DKGRDPSSDTYEEDSELLLQIRNDVLDSL-GISPDLLPEDFVR----YCFSEMAPVCAVV 306
GR P E+ +EL+ + +L + + L ED +R ++AP+ A +
Sbjct: 342 QHGRPPRPRNEEDAAELVALAQAVNARALPAVQQNNLDEDLIRKLAYVAAGDLAPINAFI 401
Query: 307 GGILAQEIVKALSQRDPPHNNFFFFDGMK 335
GG+ AQE++KA S + P + +FD ++
Sbjct: 402 GGLAAQEVMKACSGKFMPIMQWLYFDALE 430
Score = 48.5 bits (114), Expect = 9e-06
Identities = 34/141 (24%), Positives = 64/141 (45%), Gaps = 14/141 (9%)
Query: 18 QYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGV-----KGLTMLDHEQVT 72
+YD Q+ ++G + Q++L + LVG +G E+ KN + G+ + + D + +
Sbjct: 450 RYDGQVAVFGSDLQEKLGKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEIIVTDMDTIE 509
Query: 73 PEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVK-------VDTEDIEKKPESFFTQ 125
+ QFL R V + +++ + + +NP + V DTE I + FF
Sbjct: 510 KSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERI--YDDDFFQN 567
Query: 126 FDAVCLTCCSRDVIVKVDQIC 146
D V + D + +D+ C
Sbjct: 568 LDGVANALDNVDARMYMDRRC 588
>gi|38045948 ubiquitin-like modifier activating enzyme 7 [Homo
sapiens]
Length = 1012
Score = 111 bits (277), Expect = 1e-24
Identities = 65/171 (38%), Positives = 92/171 (53%), Gaps = 3/171 (1%)
Query: 19 YDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQVTPEDPGA 78
Y RQ+ + G A +R++ +RVL+ GL+GLGAE+AKNL+L GV LT+ D D A
Sbjct: 15 YSRQLYVLGSPAMQRIQGARVLVSGLQGLGAEVAKNLVLMGVGSLTLHDPHPTCWSDLAA 74
Query: 79 QFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDTEDIEKKPESFFTQFDAVCLTCCSRDV 138
QFL+ + R+RAEAS E LN V V V T DI E F V LT +
Sbjct: 75 QFLLSEQDLERSRAEASQELLAQLNRAVQVVVHTGDI---TEDLLLDFQVVVLTAAKLEE 131
Query: 139 IVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTKVAKVSQGVE 189
+KV +CHK+ + F D G G F + GE V++ T+ ++ ++
Sbjct: 132 QLKVGTLCHKHGVCFLAADTRGLVGQLFCDFGEDFTVQDPTEAEPLTAAIQ 182
Score = 63.9 bits (154), Expect = 2e-10
Identities = 41/123 (33%), Positives = 65/123 (52%), Gaps = 10/123 (8%)
Query: 17 AQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVK-----GLTMLDHEQV 71
++YD QI ++G Q++LR LLVG +G E+ K L G+ GLT++D + +
Sbjct: 413 SRYDGQIAVFGAGFQEKLRRQHYLLVGAGAIGCELLKVFALVGLGAGNSGGLTVVDMDHI 472
Query: 72 TPEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVDVKVDTEDIEKKPE-----SFFTQF 126
+ QFL R+ VGR +AE + A+ LNP + V T ++ E +FF++
Sbjct: 473 ERSNLSRQFLFRSQDVGRPKAEVAAAAARGLNPDLQVIPLTYPLDPTTEHIYGDNFFSRV 532
Query: 127 DAV 129
D V
Sbjct: 533 DGV 535
Score = 34.7 bits (78), Expect = 0.13
Identities = 12/36 (33%), Positives = 27/36 (75%)
Query: 299 MAPVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGM 334
++P+ A++G + AQE++KA+S++ P + + +FD +
Sbjct: 357 LSPMVAMLGAVAAQEVLKAISRKFMPLDQWLYFDAL 392
>gi|4502169 NEDD8 activating enzyme E1 subunit 1 isoform a [Homo
sapiens]
Length = 534
Score = 100 bits (248), Expect = 2e-21
Identities = 55/162 (33%), Positives = 84/162 (51%), Gaps = 3/162 (1%)
Query: 18 QYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQVTPEDPG 77
+YDRQ+RLWG Q+ L ++ V L+ G EI KNL+L G+ T++D QV+ ED G
Sbjct: 12 KYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAG 71
Query: 78 AQFLIRTGSVGRNRAEASLERAQNLNPMVD---VKVDTEDIEKKPESFFTQFDAVCLTCC 134
F ++ S+G+NRAEA++E Q LN V V+ E++ SFF +F V T
Sbjct: 72 NNFFLQRSSIGKNRAEAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTVVVATQL 131
Query: 135 SRDVIVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEFVE 176
+++ + + I +G GY + EH +E
Sbjct: 132 PESTSLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIE 173
Score = 48.1 bits (113), Expect = 1e-05
Identities = 44/167 (26%), Positives = 79/167 (47%), Gaps = 15/167 (8%)
Query: 181 VAKVSQGVEDGPDT---KRAKLDSSETTMVKKKVVFC-PVKEALEVDW-SSEKAKAALKR 235
VAK+ Q + P++ K KL S + ++ VV C + E +D + ++ +++
Sbjct: 361 VAKLLQSIGQAPESISEKELKLLCSNSAFLR--VVRCRSLAEEYGLDTINKDEIISSMDN 418
Query: 236 TTSD---YFLLQVLLKFRTDKGRDPSSDTY--EEDSELLLQIRNDVLDSLGIS---PDLL 287
++ Y +L+ + +F +GR P Y EED L L G+S D
Sbjct: 419 PDNEIVLYLMLRAVDRFHKQQGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVMVKDDY 478
Query: 288 PEDFVRYCFSEMAPVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGM 334
+F RY +E + A +GG AQE++K ++++ NN + + GM
Sbjct: 479 VHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGM 525
>gi|66363686 NEDD8 activating enzyme E1 subunit 1 isoform b [Homo
sapiens]
Length = 528
Score = 90.5 bits (223), Expect = 2e-18
Identities = 51/158 (32%), Positives = 79/158 (50%), Gaps = 3/158 (1%)
Query: 22 QIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQVTPEDPGAQFL 81
+ RLWG Q+ L ++ V L+ G EI KNL+L G+ T++D QV+ ED G F
Sbjct: 10 EARLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNFF 69
Query: 82 IRTGSVGRNRAEASLERAQNLNPMVD---VKVDTEDIEKKPESFFTQFDAVCLTCCSRDV 138
++ S+G+NRAEA++E Q LN V V+ E++ SFF +F V T
Sbjct: 70 LQRSSIGKNRAEAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTVVVATQLPEST 129
Query: 139 IVKVDQICHKNSIKFFTGDVFGYHGYTFANLGEHEFVE 176
+++ + + I +G GY + EH +E
Sbjct: 130 SLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIE 167
Score = 48.1 bits (113), Expect = 1e-05
Identities = 44/167 (26%), Positives = 79/167 (47%), Gaps = 15/167 (8%)
Query: 181 VAKVSQGVEDGPDT---KRAKLDSSETTMVKKKVVFC-PVKEALEVDW-SSEKAKAALKR 235
VAK+ Q + P++ K KL S + ++ VV C + E +D + ++ +++
Sbjct: 355 VAKLLQSIGQAPESISEKELKLLCSNSAFLR--VVRCRSLAEEYGLDTINKDEIISSMDN 412
Query: 236 TTSD---YFLLQVLLKFRTDKGRDPSSDTY--EEDSELLLQIRNDVLDSLGIS---PDLL 287
++ Y +L+ + +F +GR P Y EED L L G+S D
Sbjct: 413 PDNEIVLYLMLRAVDRFHKQQGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVMVKDDY 472
Query: 288 PEDFVRYCFSEMAPVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGM 334
+F RY +E + A +GG AQE++K ++++ NN + + GM
Sbjct: 473 VHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGM 519
>gi|150417996 ubiquitin-activating enzyme E1-like 2 [Homo sapiens]
Length = 1052
Score = 86.7 bits (213), Expect = 3e-17
Identities = 60/172 (34%), Positives = 85/172 (49%), Gaps = 5/172 (2%)
Query: 13 EEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQVT 72
E + A Y RQ + G A +++ S V L G+ GLG EIAKNL+LAG+K +T+ D E+
Sbjct: 38 EIDDALYSRQRYVLGDTAMQKMAKSHVFLSGMGGLGLEIAKNLVLAGIKAVTIHDTEKCQ 97
Query: 73 PEDPGAQFLIRTGSV--GRNRAEASLERAQNLNPMVDVKVDTEDIEKKPE-SFFTQFDAV 129
D G F + V RNRAEA L+ LNP V V + + + SF ++ V
Sbjct: 98 AWDLGTNFFLSEDDVVNKRNRAEAVLKHIAELNPYVHVTSSSVPFNETTDLSFLDKYQCV 157
Query: 130 CLTCCSRDVIVKVDQICHKN--SIKFFTGDVFGYHGYTFANLGEHEFVEEKT 179
LT + K++ C IKF + DV G F + G+ V + T
Sbjct: 158 VLTEMKLPLQKKINDFCRSQCPPIKFISADVHGIWSRLFCDFGDEFEVLDTT 209
Score = 39.3 bits (90), Expect = 0.005
Identities = 34/131 (25%), Positives = 67/131 (51%), Gaps = 15/131 (11%)
Query: 218 EALEVDWSSEKAKAALKRTTSDYFLLQVLLKFRTDKGRDPSSDTYEEDSELLLQIRNDVL 277
+ L VD+S+ +A + + + L +F+ R P+ ++DSE LL++ +
Sbjct: 310 KCLIVDFSNPEAPLEI------HTAMLALDQFQEKYSRKPNVGC-QQDSEELLKLATSIS 362
Query: 278 DSLGISPDLLPEDFVRYCF----SEMAPVCAVVGGILAQEIVKALSQRDPPHNNFFFFDG 333
++L PD+ D V + ++P+ A VGG+ +QE++KA++ + P + +
Sbjct: 363 ETLEEKPDV-NADIVHWLSWTAQGFLSPLAAAVGGVASQEVLKAVTGKFSPLCQWLY--- 418
Query: 334 MKGNGIVECLG 344
++ IVE LG
Sbjct: 419 LEAADIVESLG 429
Score = 39.3 bits (90), Expect = 0.005
Identities = 31/145 (21%), Positives = 59/145 (40%), Gaps = 11/145 (7%)
Query: 32 KRLRASRVLLVGLKGLGAEIAKNLILAGV-----KG-LTMLDHEQVTPEDPGAQFLIRTG 85
++L+ + LVG +G E+ KN L GV KG +T+ D + + + QFL R
Sbjct: 456 QKLQNLNIFLVGCGAIGCEMLKNFALLGVGTSKEKGMITVTDPDLIEKSNLNRQFLFRPH 515
Query: 86 SVGRNRAEASLERAQNLNPMVDVKVDTEDIEKKPES-----FFTQFDAVCLTCCSRDVIV 140
+ + ++ + + +N + + + E+ F+T+ D + + +
Sbjct: 516 HIQKPKSYTAADATLKINSQIKIDAHLNKVCPTTETIYNDEFYTKQDVIITALDNVEARR 575
Query: 141 KVDQICHKNSIKFFTGDVFGYHGYT 165
VD C N G G+T
Sbjct: 576 YVDSRCLANLRPLLDSGTMGTKGHT 600
>gi|4885649 SUMO-1 activating enzyme subunit 2 [Homo sapiens]
Length = 640
Score = 57.8 bits (138), Expect = 1e-08
Identities = 38/138 (27%), Positives = 63/138 (45%), Gaps = 4/138 (2%)
Query: 29 EAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQVTPEDPGAQFLIRTGSVG 88
E + + RVL+VG G+G E+ KNL+L G + ++D + + + QFL + VG
Sbjct: 10 ELAEAVAGGRVLVVGAGGIGCELLKNLVLTGFSHIDLIDLDTIDVSNLNRQFLFQKKHVG 69
Query: 89 RNRAEASLERAQNLNPMVDVKVDTEDIEKKPE---SFFTQFDAVCLTCCSRDVIVKVDQI 145
R++A+ + E P ++ V D P+ FF QF V +R V+++
Sbjct: 70 RSKAQVAKESVLQFYPKANI-VAYHDSIMNPDYNVEFFRQFILVMNALDNRAARNHVNRM 128
Query: 146 CHKNSIKFFTGDVFGYHG 163
C + GY G
Sbjct: 129 CLAADVPLIESGTAGYLG 146
>gi|38045944 ubiquitin-activating enzyme 3 isoform 2 [Homo sapiens]
Length = 449
Score = 55.8 bits (133), Expect = 5e-08
Identities = 32/107 (29%), Positives = 55/107 (51%), Gaps = 3/107 (2%)
Query: 34 LRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQVTPEDPGAQFLIRTGSVGRNRAE 93
L +VL++G GLG E+ KNL L+G + + ++D + + + QFL R +GR +AE
Sbjct: 53 LDTCKVLVIGAGGLGCELLKNLALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDIGRPKAE 112
Query: 94 ASLERAQNLNPMVDVKVDTEDIEKKPESFFTQFDAVCLTCCSRDVIV 140
+ E + P +V I+ ++F+ QF + C D I+
Sbjct: 113 VAAEFLNDRVPNCNVVPHFNKIQDFNDTFYRQFHII---VCGLDSII 156
>gi|38045942 ubiquitin-activating enzyme 3 isoform 1 [Homo sapiens]
Length = 463
Score = 55.8 bits (133), Expect = 5e-08
Identities = 32/107 (29%), Positives = 55/107 (51%), Gaps = 3/107 (2%)
Query: 34 LRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQVTPEDPGAQFLIRTGSVGRNRAE 93
L +VL++G GLG E+ KNL L+G + + ++D + + + QFL R +GR +AE
Sbjct: 67 LDTCKVLVIGAGGLGCELLKNLALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDIGRPKAE 126
Query: 94 ASLERAQNLNPMVDVKVDTEDIEKKPESFFTQFDAVCLTCCSRDVIV 140
+ E + P +V I+ ++F+ QF + C D I+
Sbjct: 127 VAAEFLNDRVPNCNVVPHFNKIQDFNDTFYRQFHII---VCGLDSII 170
>gi|7657339 molybdenum cofactor synthesis 3 [Homo sapiens]
Length = 460
Score = 48.1 bits (113), Expect = 1e-05
Identities = 32/99 (32%), Positives = 52/99 (52%), Gaps = 2/99 (2%)
Query: 11 ISEEEAAQYDRQIRL--WGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDH 68
+S +E +Y RQ+ L G+ Q RL + VL+VG GLG +A+ L AGV L ++D+
Sbjct: 55 LSRDEILRYSRQLVLPELGVHGQLRLGTACVLIVGCGGLGCPLAQYLAAAGVGRLGLVDY 114
Query: 69 EQVTPEDPGAQFLIRTGSVGRNRAEASLERAQNLNPMVD 107
+ V + Q L G+ +A ++ + LN V+
Sbjct: 115 DVVEMSNLARQVLHGEALAGQAKAFSAAASLRRLNSAVE 153
>gi|66363688 NEDD8 activating enzyme E1 subunit 1 isoform c [Homo
sapiens]
Length = 445
Score = 48.1 bits (113), Expect = 1e-05
Identities = 44/167 (26%), Positives = 79/167 (47%), Gaps = 15/167 (8%)
Query: 181 VAKVSQGVEDGPDT---KRAKLDSSETTMVKKKVVFC-PVKEALEVDW-SSEKAKAALKR 235
VAK+ Q + P++ K KL S + ++ VV C + E +D + ++ +++
Sbjct: 272 VAKLLQSIGQAPESISEKELKLLCSNSAFLR--VVRCRSLAEEYGLDTINKDEIISSMDN 329
Query: 236 TTSD---YFLLQVLLKFRTDKGRDPSSDTY--EEDSELLLQIRNDVLDSLGIS---PDLL 287
++ Y +L+ + +F +GR P Y EED L L G+S D
Sbjct: 330 PDNEIVLYLMLRAVDRFHKQQGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVMVKDDY 389
Query: 288 PEDFVRYCFSEMAPVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGM 334
+F RY +E + A +GG AQE++K ++++ NN + + GM
Sbjct: 390 VHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGM 436
>gi|222144229 APG7 autophagy 7-like isoform c [Homo sapiens]
Length = 623
Score = 37.0 bits (84), Expect = 0.026
Identities = 31/123 (25%), Positives = 58/123 (47%), Gaps = 6/123 (4%)
Query: 38 RVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQVTPEDPGAQFLIRTGSV---GRNRAEA 94
+ LL+G LG +A+ L+ GV+ +T +D+ +++ +P Q L G+ +A A
Sbjct: 318 KCLLLGAGTLGCNVARTLMGWGVRHITFVDNAKISYSNPVRQPLYEFEDCLGGGKPKALA 377
Query: 95 SLERAQNLNPMVDVKVDTEDIEKKPESFFTQFDAVCLTCCSRDVIVKVDQICHKNSIKFF 154
+ +R Q + P V+ + I F +V L RDV +++Q+ + + F
Sbjct: 378 AADRLQKIFPGVNARGFNMSIPMPGHP--VNFSSVTLEQARRDV-EQLEQLIESHDVVFL 434
Query: 155 TGD 157
D
Sbjct: 435 LMD 437
>gi|222144227 APG7 autophagy 7-like isoform b [Homo sapiens]
Length = 676
Score = 37.0 bits (84), Expect = 0.026
Identities = 31/123 (25%), Positives = 58/123 (47%), Gaps = 6/123 (4%)
Query: 38 RVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQVTPEDPGAQFLIRTGSV---GRNRAEA 94
+ LL+G LG +A+ L+ GV+ +T +D+ +++ +P Q L G+ +A A
Sbjct: 357 KCLLLGAGTLGCNVARTLMGWGVRHITFVDNAKISYSNPVRQPLYEFEDCLGGGKPKALA 416
Query: 95 SLERAQNLNPMVDVKVDTEDIEKKPESFFTQFDAVCLTCCSRDVIVKVDQICHKNSIKFF 154
+ +R Q + P V+ + I F +V L RDV +++Q+ + + F
Sbjct: 417 AADRLQKIFPGVNARGFNMSIPMPGHP--VNFSSVTLEQARRDV-EQLEQLIESHDVVFL 473
Query: 155 TGD 157
D
Sbjct: 474 LMD 476
>gi|5453668 APG7 autophagy 7-like isoform a [Homo sapiens]
Length = 703
Score = 37.0 bits (84), Expect = 0.026
Identities = 31/123 (25%), Positives = 58/123 (47%), Gaps = 6/123 (4%)
Query: 38 RVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQVTPEDPGAQFLIRTGSV---GRNRAEA 94
+ LL+G LG +A+ L+ GV+ +T +D+ +++ +P Q L G+ +A A
Sbjct: 357 KCLLLGAGTLGCNVARTLMGWGVRHITFVDNAKISYSNPVRQPLYEFEDCLGGGKPKALA 416
Query: 95 SLERAQNLNPMVDVKVDTEDIEKKPESFFTQFDAVCLTCCSRDVIVKVDQICHKNSIKFF 154
+ +R Q + P V+ + I F +V L RDV +++Q+ + + F
Sbjct: 417 AADRLQKIFPGVNARGFNMSIPMPGHP--VNFSSVTLEQARRDV-EQLEQLIESHDVVFL 473
Query: 155 TGD 157
D
Sbjct: 474 LMD 476
>gi|38327032 ubiquitin-activating enzyme 5 isoform 2 [Homo sapiens]
Length = 348
Score = 34.7 bits (78), Expect = 0.13
Identities = 21/84 (25%), Positives = 44/84 (52%), Gaps = 1/84 (1%)
Query: 32 KRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQVTPEDPGAQFLIRTGSVGRNR 91
+++R V +VG+ G+G+ A+ L G+ L + D+++V + F + G ++
Sbjct: 13 EKIRTFAVAIVGVGGVGSVTAEMLTRCGIGKLLLFDYDKVELANMNRLF-FQPHQAGLSK 71
Query: 92 AEASLERAQNLNPMVDVKVDTEDI 115
+A+ +N+NP V +V +I
Sbjct: 72 VQAAEHTLRNINPDVLFEVHNYNI 95
>gi|13376212 ubiquitin-activating enzyme 5 isoform 1 [Homo sapiens]
Length = 404
Score = 34.7 bits (78), Expect = 0.13
Identities = 21/84 (25%), Positives = 44/84 (52%), Gaps = 1/84 (1%)
Query: 32 KRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQVTPEDPGAQFLIRTGSVGRNR 91
+++R V +VG+ G+G+ A+ L G+ L + D+++V + F + G ++
Sbjct: 69 EKIRTFAVAIVGVGGVGSVTAEMLTRCGIGKLLLFDYDKVELANMNRLF-FQPHQAGLSK 127
Query: 92 AEASLERAQNLNPMVDVKVDTEDI 115
+A+ +N+NP V +V +I
Sbjct: 128 VQAAEHTLRNINPDVLFEVHNYNI 151
>gi|193788653 hypothetical protein LOC100131897 [Homo sapiens]
Length = 535
Score = 30.8 bits (68), Expect = 1.9
Identities = 24/69 (34%), Positives = 34/69 (49%), Gaps = 4/69 (5%)
Query: 168 NLGEHEFVEEKTKVAKVSQGVEDGPDTKRAKLDSSETTMVKKKVVFCPVKEALEV-DWSS 226
NL E + V +++Q +EDGP +R K+ + V K PV LE W S
Sbjct: 137 NLSEQDIVSSDLAYLRLAQHLEDGP--RRVKVSHAFLPRVPKVQSNGPVSICLEAGTWRS 194
Query: 227 -EKAKAALK 234
EKA AA++
Sbjct: 195 LEKATAAIQ 203
>gi|109809739 La ribonucleoprotein domain family, member 7 [Homo
sapiens]
Length = 582
Score = 29.6 bits (65), Expect = 4.1
Identities = 25/91 (27%), Positives = 41/91 (45%), Gaps = 3/91 (3%)
Query: 175 VEEKTKVAKVSQGV--EDGPDTKRAKLDSSETTMVKKKVVFCPVKEALEVDWSSEKAKAA 232
VEEK K K + ED K +D+S T++ K K P E +++ + + + +
Sbjct: 215 VEEKKKKKKKKGRMKKEDNIQAKEENMDTSNTSISKMK-RSRPTSEGSDIESTEPQKQCS 273
Query: 233 LKRTTSDYFLLQVLLKFRTDKGRDPSSDTYE 263
K+ D L + RT K + SS+ E
Sbjct: 274 KKKKKRDRVEASSLPEVRTGKRKRSSSEDAE 304
>gi|109809741 La ribonucleoprotein domain family, member 7 [Homo
sapiens]
Length = 582
Score = 29.6 bits (65), Expect = 4.1
Identities = 25/91 (27%), Positives = 41/91 (45%), Gaps = 3/91 (3%)
Query: 175 VEEKTKVAKVSQGV--EDGPDTKRAKLDSSETTMVKKKVVFCPVKEALEVDWSSEKAKAA 232
VEEK K K + ED K +D+S T++ K K P E +++ + + + +
Sbjct: 215 VEEKKKKKKKKGRMKKEDNIQAKEENMDTSNTSISKMK-RSRPTSEGSDIESTEPQKQCS 273
Query: 233 LKRTTSDYFLLQVLLKFRTDKGRDPSSDTYE 263
K+ D L + RT K + SS+ E
Sbjct: 274 KKKKKRDRVEASSLPEVRTGKRKRSSSEDAE 304
>gi|189027141 abhydrolase domain containing 6 [Homo sapiens]
Length = 337
Score = 28.5 bits (62), Expect = 9.2
Identities = 19/77 (24%), Positives = 39/77 (50%), Gaps = 10/77 (12%)
Query: 257 PSSDTYEEDSELLLQIR----NDVLDSLGISPDLLPEDFVRYCFSEMAPVCAVVGGILAQ 312
P+ Y D++ + +++ + ++ + + P PE+ SEM +C+ V + Q
Sbjct: 173 PAGLQYSTDNQFVQRLKELQGSAAVEKIPLIPST-PEEM-----SEMLQLCSYVRFKVPQ 226
Query: 313 EIVKALSQRDPPHNNFF 329
+I++ L PHNNF+
Sbjct: 227 QILQGLVDVRIPHNNFY 243
>gi|104526627 triadin [Homo sapiens]
Length = 729
Score = 28.5 bits (62), Expect = 9.2
Identities = 14/36 (38%), Positives = 23/36 (63%)
Query: 175 VEEKTKVAKVSQGVEDGPDTKRAKLDSSETTMVKKK 210
V + K A+VS+ VED P +K+AK + + + K+K
Sbjct: 648 VTKAEKPARVSKDVEDVPASKKAKEGTEDVSPTKQK 683
>gi|40805823 collagen, type XXII, alpha 1 [Homo sapiens]
Length = 1626
Score = 28.5 bits (62), Expect = 9.2
Identities = 21/66 (31%), Positives = 33/66 (50%), Gaps = 2/66 (3%)
Query: 256 DPSSDTYEEDSELLLQIRNDVLDSLGISPDLLPEDFVRYCFSEMAPVCAVVGGILAQEIV 315
D SS +ED E + Q +++D+ + PD VRY S+ +G +QE V
Sbjct: 44 DTSSSVGKEDFEKVRQWVANLVDTFEVGPDRTRVGVVRY--SDRPTTAFELGLFGSQEEV 101
Query: 316 KALSQR 321
KA ++R
Sbjct: 102 KAAARR 107
Database: hs.faa
Posted date: Aug 4, 2009 4:42 PM
Number of letters in database: 18,247,518
Number of sequences in database: 37,866
Lambda K H
0.318 0.136 0.393
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,878,216
Number of Sequences: 37866
Number of extensions: 540967
Number of successful extensions: 1532
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 19
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 1489
Number of HSP's gapped (non-prelim): 40
length of query: 346
length of database: 18,247,518
effective HSP length: 103
effective length of query: 243
effective length of database: 14,347,320
effective search space: 3486398760
effective search space used: 3486398760
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 62 (28.5 bits)
Search results were obtained with NCBI BLAST and RefSeq entries.
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Guide to the Human Genome
Copyright © 2010 by Stewart Scherer. All rights reserved.